본문 바로가기
KERD
논문KCI 등재

국내 가축 유래 대장균에서 CTX-M 및 TEM형 extended-spectrum β-lactamases의 검출

Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea

김진현|김기석|조재근|성명숙
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2011.03|한국동물위생학회지|제34권 제1호|pp. 37-43

2

조회

0

다운로드

3

인용

0

참고문헌

키워드

Escherichia coliESBLbla_TEM-1bla_TEM-116bla_CTX-15
주제분류수의학

초록

This study was conducted to investigate the prevalence and genotypes of extended-spectrum β-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3∼4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both bla_CTX-15 and bla_TEM-1, and one isolate from chicken have bla_CTX-15 and bla_TEM-116. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that bla_TEM-116 gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.

Abstract

This study was conducted to investigate the prevalence and genotypes of extended-spectrum β-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3∼4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both bla_CTX-15 and bla_TEM-1, and one isolate from chicken have bla_CTX-15 and bla_TEM-116. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that bla_TEM-116 gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.

참고문헌 (32)

  1. 성명숙, "국내 가금유래 병원성 대장균의 extended-spectrum β-lactamase(ESBL) 특성 조사", 대한수의학회지, 48(3), pp.259~265 (2008), 대한수의학회
  2. 정병렬, "국내 동물 유래 extended-spectrum", 한국수의공중보건학회지, 34(2), pp.103~108 (2010)
  3. Ambler RP, "A standard numbering scheme for the class A beta-lactamases", Biochem J, 273, pp.269~270 (1991)
  4. Bauernfeind A, "A new plasmidic cefotaximase in a clinical isolate of Escherichia coli", Infection, 18(5), pp.294~298 (1990)
  5. Bonnet R, "Growing group of extended-spectrum b-lactamases: The CTX-M- enzymes", Antimicrob Agents Chemother, 48(1), pp.1~14 (2004)
  6. Bradford PA, "Extended-spectrum b-lactamases in the 21st century: Characterization, epidemiology, and detection of this important resistance threat", Clin Microbiol Rev, 14(4), pp.933~951 (2001)
  7. Bradley DE, "Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12", J Bacteriol, 143(3), pp.1466~1470 (1980)
  8. Briñas L, "b-lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals", Antimicrob Agents Chemother, 46(10), pp.3156~3163 (2002)
  9. Briñas L, "Monitoring and characterization of extended-spectrum beta-lactamases in Escherichia coli strains from healthy and sick animals in Spain in 2003", Antimicrob Agents Chemother, 49(3), pp.1262~1264 (2003)
  10. Bush K, "A functional classification scheme for beta-lactamases and its correlation with molecular structure", Antimicrob Agents Chemother, 39(6), pp.1211~1233 (1995)
  11. Carattoli A, "Extended-spectrum beta-lactamases in Escherichia coli isolated from dogs and cats in Rome, Italy, from 2001 to 2003", Antimicrob Agents Chemother, 49(2), pp.833~835 (2003)
  12. "Standardized laboratory protocol for molecular subtyping of Escherchia coli O157:H7, non- typhoidal Salmonella serotypes, and Shigella sonnei by PFGE" (2004)[URL]
  13. Jacoby GA, "More extended-spectrum beta-lactamases", Antimicrob Agents Chemother, 35(9), pp.1697~1704 (1991)
  14. Jarlier V, "Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns", Rev Infect Dis, 10(4), pp.867~878 (1988)
  15. Jeong SH, "Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey", J Clin Microbiol, 42(7), pp.2902~2906 (2004)
  16. Kim J, "Survey of Klebsiella pneumoniae strains producing extended- spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea", J Clin Microbiol, 36(5), pp.1446~1449 (1998)
  17. Kim J, "Occurrence of CTX-M-3, CTX-M-15, CTX-M-14, and CTX-M-9 extended-spectrum b-lactamases in Enterobacteriae clinical isolates in Korea", Antimicrob Agents Chemother, 49(4), pp.1572~1575 (2005)
  18. Kim J, "CTX-M and SHV-12 b-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pnemoniae collected from 3 university hospitals within Korea", FEMS Microbiol Let, 245(1), pp.93~98 (2005)
  19. Knothe H, "Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens", Infection, 11(6), pp.315~317 (1983)
  20. Lim SK, "CTX- M-type beta-lactamase in Escherichia coli isolated from sick animals in Korea", Microb Drug Resist, 15(2), pp.139~142 (2009)
  21. Livermore DM, "b-lactamases in laboratory and clinical resistance", Clin. Microbiol Rev, 8(4), pp.557~584 (1995)
  22. Meunier D, "CTX-M-1 and CTX-M-15-type beta-lactamases in clinical Escherichia coli isolates recovered from food-producing animals in France", Int J Antimicrob Agents, 28(5), pp.402~407 (2006)
  23. Monstein HJ, "Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae", APMIS, 115(2), pp.1400~1408 (2007)
  24. National Committee for Clinical Laboratory Standards, "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ; Approved standard-sixth edition" (2003), NCCLS document M7-A. National Committee for Clinical Laboratory Standards
  25. National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial disk susceptibility test, 8th infomational supplement: approved standard M2-A8" (2003), NCCLS
  26. Oliver A, "Ampicillin-sulbactam and amoxicillin-clavulanate susceptibility testing of Escherichia coli isolates with different beta-lactam resistance phenotypes", Antimicrob Agents Chemother, 43(4), pp.862~767 (1999)
  27. Pai H, "Identification of CTX-M-14 extended-spectrum beta-lactamase in clinical isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea", J Clin Microbiol, 39(10), pp.3747~3749 (2001)
  28. Pai H, "Survey of extended-spectrum b-lactamase in clinical isolates of Escherichia coli and Klebsiella pneumoniae : prevalence of TEM-52 in Korea", J Clin Microbiol, 37(6), pp.1758~1763 (1999)
  29. 박남용, "Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip", 한국가축위생학회지, 30(4), pp.505~510 (2007), 한국가축위생학회
  30. Paterson DL, "Extended-spectrum beta-lactamases: a clinical update", Clin Microbiol Rev, 18(4), pp.657~686 (2005)
  31. Poirel L, "Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3", J Antimicrob Chemother, 50(6), pp.1031~1034 (2002)
  32. Tzouvelekis LS, "CTX-M-type beta-lactamases: an emerging group of extended-spectrum enzymes", Int J Antimicrob Agents, 14(2), pp.137~142 (2000)

관련 논문AI추천

Characteristics of Transmissible CTX-M- and CMY-Type β-Lactamase- Producing Escherichia coli Isolates Collected from Pig and Chicken Farms in South Korea신승원, 원호근, 유한상, 윤인중, 정명환, Kuastros Mekonnen Belaynehe한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제9호Prevalence of the Extended-Spectrum β-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli어영, 강현경, 김재석, 김주원, 박용정, 배일권, 이경원, 정석훈대한진단검사의학회 · Annals of Laboratory Medicine2009 · 제29권 제3호대한민국 청주 지역사회의 CTX-M Extended-spectrum β-lactamase 생성 대장균주의 확산김은선, 김혜연, 성지연, 손자민, 오지은, 임다영대한임상검사과학회 · 대한임상검사과학회지2013 · 제45권 제2호CTX-M형 Extended-Spectrum -Lactamase 생성 Escherichia coli의 확산 및 CTX-M-12의 출현어영, 강정옥, 곽효선, 김의종, 박연준, 배일권, 송은향, 신종희, 안지영, 오지은, 용동은, 우건조, 이경원, 이위교, 이종욱, 장석훈, 홍성근, 홍종식대한진단검사의학회 · Annals of Laboratory Medicine2005 · 제25권 제4호Ambler Class A Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의국내분리현황어영, 강정옥, 강지혜, 권수봉, 김의종, 박연준, 배일권, 신종희, 안지영, 용동은, 우건조, 이경원, 이위교, 이종욱, 정석훈, 홍성근대한임상미생물학회 · Annals of Clinical Microbiology2005 · 제8권 제1호대구지역 폐수처리장에서 분리한 cefotaxime-resistant Escherichia coli의 특성김은정, 김환득, 박대현, 이미리, 조재근한국동물위생학회 · 한국동물위생학회지2014 · 제37권 제4호

이 간행물의 다른 논문 (12건)

Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2박철, 김용환, 김범석, 조호성pp. 1-4IBDV에 대한 단크론항체 생산 및 진단적 응용류민상, 송윤기, 모인필, 이승철, 강신영pp. 5-12돼지 써코바이러스 2형 유전형 분류를 위한 nested-PCR 적용추금숙pp. 13-18전북지역에서 2004∼2008년에 닭과 오리에서 법정전염병 발생동향 분석이정원, 송희종, 허부홍pp. 19-29Clostridium perfringens 감염에 의한 한우 집단 폐사 증례손구례, 김지영, 고원석, 곽길한, 이정원, 육현수pp. 31-35
원문 보기