소 브루셀라병 동시 다두 발생 농장의 발생 요인 조사
Investigation of occurrence factors on brucellosis-outbreak farm in Korea
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참고문헌
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초록
From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.
Abstract
From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.
참고문헌 (20)
- Banai M., "Taxonomy of Brucella", The Open Vet Sci J, 4, pp.85~101 (2010)
- Her M, "Outbreak of brucellosis in domestic elk in Korea", Zoonoses Public Health, 57, pp.155~161 (2010)
- Her M., "Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea", BMC Microbiol, 9, pp.230~ (2009)
- Jain N., "Effect of exogenous erythritol on growth and survival of Brucella", Vet Microbiol, 160, pp.513~516 (2012)
- Jung SC., "Bovine brucellosis in Korea", J Zoonoses, 1, pp.1~15 (2010)
- Kang SI., "Advanced multiplex PCR assay for differentiationof Brucella species", Appl Environ Microbiol, 77, pp.6726~6728 (2011)
- Kim ST., "Brucellosis outbreak of Korean indigenous cattleat Yeongwol and Pyeongchang county in Korea", KoreanJ Vet Serv, 28, pp.387~392 (2005)
- Kim SY., "A serological survey on large outbreak of bovine brucellosis in dairy farm", Korean J Vet Serv, 22, pp.159~167 (1999)
- Lee BY., "Surveillance and control of bovine brucellosis in the Republic of Korea during 2000-2006", Prev Vet Med, 90, pp.66~79 (2006)
- Martirosyan A., "An evolutionary strategy for a stealthy intracellular Brucella pathogen", Immunol Rev, 240, pp.211~234 (2011)
- Meador VP., "Intracellular localization of Brucella abortus in bovine placenta", Vet Pathol, 26, pp.513~515 (1989)
- Nicoletti P., "The epidemiology of bovine brucellosis", Adv Vet Sci Comp Med, 24, pp.69~98 (1980)
- Park NC., "Studies on the brucellosis in Kyongbuk area", Korean J Vet Serv, 21, pp.451~465 (1999)
- Prior MG., "Isolation of Brucella abortus from two dogs in contact with bovine brucellosis", Can J comp Med, 40, pp.117~118 (1976)
- 류재윤, "브루셀라병 혈청검사 양성 수소와 12개월령 이하 소에서 균의 분리 및 동정", 한국가축위생학회지, 30(3), pp.375~384 (2007), 한국가축위생학회
- Seleem MN., "Brucella: a pathogen without classic virulence genes", Vet Microbiol, 129, pp.1~14 (2008)
- Timoney JF., "Hagan and Bruner's microbiology and infectious diseases of domestic animals", pp.135~152 (1988), Cornell University Press
- Troung LQ., "Epidemiological survey for Brucella in wildlife and stray dogs, a cat and rodents captured on farms", J Vet Med Sci, 73, pp.1597~1601 (2011)
- 심항섭, "Bacteriological detection of Brucella abortus and its characterization by PCR in the sporadic outbreak of bovine brucellosis in Gyeonggi province", 한국가축위생학회지, 30(2), pp.251~258 (2007), 한국가축위생학회
- Yoon YB., "Epidemiological study for infection route of brucellosis in a infected dairy farms", Korean J Vet Serv, 27, pp.159~164 (2004)