등온증폭법을 이용한 돼지인플루엔자바이러스 신속 진단법 개발
Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus
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In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that acci-dentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/L) and the same to real time RT-PCR (1 copy/L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diag-nostic laboratory in the future.
Abstract
In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that acci-dentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/L) and the same to real time RT-PCR (1 copy/L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diag-nostic laboratory in the future.
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