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등온증폭법을 이용한 돼지인플루엔자바이러스 신속 진단법 개발

Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus

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초록

In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that acci-dentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/L) and the same to real time RT-PCR (1 copy/L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diag-nostic laboratory in the future.

Abstract

In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that acci-dentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/L) and the same to real time RT-PCR (1 copy/L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diag-nostic laboratory in the future.

참고문헌 (33)

  1. Chander Y, "A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification(LAMP)", Front Microbiol, 5, pp.395~ (2014)
  2. 조애리, "오리 도체에서 등온유전자증폭기법을 이용한Salmonella spp. 신속 고감도 검출 기법 연구", 한국축산식품학회지, 33(5), pp.655~663 (2013), 한국축산식품학회
  3. Cho HS, "Detection of canine distemper virus in blood samples by reverse transcription loop-mediated isothermal amplification", J Vet Med B, 52, pp.410~413 (2005)
  4. Cong YL, "Antigenic and genetic characterization of H9N2 swine influenza viruses in China", J Gen Virol, 88, pp.2035~2041 (2007)
  5. Dhama K, "Loop-mediated isothermal amplification of DNA(LAMP) : a new diagnostic tool lights the world of diagnosis of animal and human pathogens : a review", Pak J Biol Sci, 17, pp.151~166 (2014)
  6. 동희진, "Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle", Journal of Veterinary Science, 15(2), pp.317~325 (2014), 대한수의학회[DOI]
  7. Gandelman O, "Loop-mediated amplification accelerated by stem primers", Int J Mol Sci, 12, pp.9108~9124 (2011)
  8. Gu H, "Rapid and specific detection of H3 swine influenza virus using reverse transcription loop-mediated isothermal amplification method", J Applied Microbiol, 108, pp.1145~1154 (2010)
  9. Hass J, "The role of swine as"mixing vessel"for interspecies transmission of he influenza A subtype H1N1 : a simultaneous Bayesian inference of phylogeny and ancestral hosts", Infect Genet Evol, 11, pp.437~441 (2011)
  10. Hoffmann, E, "Universal primer set for the full-length amplification of all influenza A viruses", Arch Virol, 146, pp.2275~2289 (2001)
  11. 황은숙, "한우 결핵의 신속 감별진단을 위한 등온증폭법 개발", 한국가축위생학회지, 34(4), pp.333~339 (2011), 한국가축위생학회
  12. Karasin AI, "Isolation and characterization of H4N6 avian influenza viruses from pigs with pneumonia in Canada", J Virol, 74, pp.9322~9327 (2000)
  13. Karasin AI, "Characterization of avian H3N3 and H1N1 influenza A viruses isolated from pigs in Canada", J Clin Microbiol, 42, pp.4349~4354 (2004)
  14. Kim HR, "Application of real-time reverse transcription polymerase chain reaction to the detection the matrix, H5 and H7 genes of avian influenza viruses in field samples from South Korea", Virol J, 10, pp.85~ (2013)
  15. Kim SH, "Outbreak of pandemic influenza (H1N1) 2009 in pigs in Korea", Vet Rec, 169, pp.155a~ (2011)
  16. Kim SH, "A novel reassortant H1N2 virus related to the pandemic H1N1 2009 influenza virus isolated from Korean pigs", Virus Genes, 48, pp.193~198 (2014)
  17. 고바라다, "Mycobacterium bovis와 M. tuberculosis 감별을 위한 등온증폭법", 한국가축위생학회지, 36(2), pp.79~86 (2013), 한국가축위생학회
  18. Lekcharoensuk P, "Novel swine influenza virus subtype H3N1, United States", Emerg Infect Dis, 12, pp.787~794 (2006)
  19. Lee MS, "Identification and subtyping of avian influenza viruses by reverse transcription-PCR", J Virol Methods, 97, pp.13~22 (2001)
  20. Liu Y, "Development and application of a rapid detection system for swine influenza virus by a reverse transcription loop-mediated isothermal amplification assay", J Food Agri Environ, 10, pp.196~200 (2012)
  21. Ma XJ, "Visual detection of pandemic influenza A H1N1 virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye", J Virol Methods, 167, pp.214~217 (2010)
  22. Notomi T, "Loop-mediated isothermal amplification of DNA", Nucleic Acids Res, 28, pp.63~69 (2000)
  23. "Manual of diagnostic test and Vaccines for terrestrial Animals"[URL]
  24. Parida M, "Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for rapid and real-time detection of the swine-origin influenza A H1N1 virus", J Mol Diagn, 13, pp.100~107 (2011)
  25. Poon LLM, "Detection of human influenza A viruses by loop-mediated isothermal amplification", J Clin Microbiol, 43, pp.427~430 (2005)
  26. Phipps LP, "Genetic subtyping of influenza A viruses using RT-PCR with a single set of primers based on conserved sequences within the HA2 coding region", J Virol Methods, 122, pp.119~122 (2004)
  27. Sakurai A, "Updated values for molecular diagnosis for highly pathogenic avian influenza virus", Viruses, 4, pp.1235~1257 (2012)
  28. Shin YK, "One-step multiplex reverse-transcriptase PCR for detecting pandemic(H1N1)2009 influenza virus", J Vet Med Sc, 73, pp.55~63 (2011)
  29. Van Reeth K, "Diseases of Swine", pp.557~571 (2012), Wiley-Blackwell Publishing
  30. Vincent A, "Review of influenza A virus in swine worldwide : a call for increased surveillance and research", Zoonoses Public Health, 61, pp.4~17 (2014)
  31. Vijaykrishna D, "Reassortment of pandemic H1N1", 2009 influenza A virus in swine, 2010, pp.328, 1529~
  32. "Pandemic (H1N1)2009-update100"[URL]
  33. Yoo MS, "Reverse transcription loop-mediated isothermal amplification for sensitive and rapid detection of Korean sacbrood virus", J Virol Methods, 186, pp.147~151 (2012)

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