Identification of Salmonella spp. from porcine salmonellosis by matrix-assisted laser desorption ionization-time of flight mass spectrometry
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrome-try (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.
Abstract
A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrome-try (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.
참고문헌 (21)
- Abubakar I, "A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food", Health Technol Assess, 11, pp.1~216 (2007)
- Anderson NW, "Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry", J Clin Microbiol, 50, pp.1008~1013 (2012)[DOI]
- Baggesen DL, "Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing", Prev Vet Med, 26, pp.201~213 (1996)[DOI]
- Brown CC, "Jubb, Kennedy, and Palmer's Pathology of Domestic Animals Vol 2", pp.193~199 (2007), Saunders Elsevier
- Chiu TH, "Development of PCR primers for the detection of Salmonella enterica serovar Choleraesuis based on the fliC gene", J Food Prot, 68, pp.1575~1580 (2005)[DOI]
- Emonet S, "Application and use of various mass spectrometry methods in clinical microbiology", Clin Microbiol Infect, 16, pp.1604~1613 (2010)[DOI]
- Griffith RW, "Diseases of Swine", pp.739~754 (2006), Blackwell Publishing
- Hsieh SY, "Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS", Mol Cell Proteomics, 7, pp.448~456 (2008)[DOI]
- Kim HJ, "Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 Salmonella genomic sequences, and genotyping of salmonellae by using PCR", Appl Environ Microbiol, 72, pp.6142~6151 (2006)[DOI]
- Lay JO Jr, "MALDI-TOF mass spectrometry of bacteria", Mass Spectrom Rev, 20, pp.172~194 (2001)[DOI]
- Mellmann A, "Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria", J Clin Microbiol, 46, pp.1946~1954 (2008)[DOI]
- Saffert RT, "Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli", J Clin Microbiol, 49, pp.887~892 (2011)[DOI]
- Sauer S, "Mass spectrometry tools for the classification and identification of bacteria", Nat Rev Microbiol, 8, pp.74~82 (2010)[DOI]
- Seng P, "Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry", Clin Infect Dis, 49, pp.543~551 (2009)[DOI]
- 손준형, "돼지유래 Salmonella속 균의 동정을 위한 MALDI TOF MS 활용", 한국동물위생학회지, 39(4), pp.247~251 (2016), 한국동물위생학회
- Sparbier K, "Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS", Eur J Clin Microbiol Infect Dis, 31, pp.767~773 (2012)[DOI]
- van Duijkeren E, "Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001", J Clin Microbiol, 40, pp.3980~3985 (2002)[DOI]
- van Veen SQ, "High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories", J Clin Microbiol, 48, pp.900~907 (2010)[DOI]
- Welker M, "Applications of whole-cell matrix-assisted laser-desorption", ionization time-of-flight mass spectrometry in systematic microbiology, 2011, pp.34, 2~11
- Widjojoatmodjo MN, "Evaluation of the magnetic immuno PCR assay for rapid detection of Salmonella", Eur J Clin Microbiol Infect Dis, 10, pp.935~938 (1991)[DOI]
- Wilcock BP, "The significance of the serotype in the clinical and pathological features of naturally occurring porcine salmonellosis", Can J Comp Med, 40, pp.80~88 (1976)