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광주광역시 꿀벌질병 동향조사

Prevalence of honeybee (Apis mellifera) diseases in Gwangju

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초록

This study was carried out to investigate the prevalence of honeybee (Apis mellifera) diseases in Gwangju area. From November 2016 to August 2017, 89 samples were collected from 33 apiculture farms and reverse transcriptase-polymerase chain reaction (RT-PCR), polymerase chain reaction (PCR), and real time PCR were conducted. 14 infectious pathogens, including seven viruses, two bacteria, three fungi, and two parasites, were investigated from random apiculture farms in Gwangju. The percentage of infectious pathogens were as follows: Stonebrood (76.4%), Deformed wing virus (51.7%), Nosema (27.0%) in PCR and RT-PCR. This result indicated that Stonebrood was most prevalent disease in Gwangju area. And we could get similar results from real time PCR. 84.8% of farms have more than two of infectious pathogens. Stonebrood and Deformed wing virus were major diseases in almost all seasons and Black queen cell virus disease was especially prevalent in May.

Abstract

This study was carried out to investigate the prevalence of honeybee (Apis mellifera) diseases in Gwangju area. From November 2016 to August 2017, 89 samples were collected from 33 apiculture farms and reverse transcriptase-polymerase chain reaction (RT-PCR), polymerase chain reaction (PCR), and real time PCR were conducted. 14 infectious pathogens, including seven viruses, two bacteria, three fungi, and two parasites, were investigated from random apiculture farms in Gwangju. The percentage of infectious pathogens were as follows: Stonebrood (76.4%), Deformed wing virus (51.7%), Nosema (27.0%) in PCR and RT-PCR. This result indicated that Stonebrood was most prevalent disease in Gwangju area. And we could get similar results from real time PCR. 84.8% of farms have more than two of infectious pathogens. Stonebrood and Deformed wing virus were major diseases in almost all seasons and Black queen cell virus disease was especially prevalent in May.

참고문헌 (20)

  1. 유미선, "꿀벌 질병 검출을 위한 real-time PCR 진단법의 개발", 한국양봉학회학술대회 자료집, pp.33~33 (2009)
  2. 현방훈, "2017 작은벌집딱정벌레 예방 및 관리", pp.6~8 (2017), 농림축산검역본부, 농촌진흥청, 국립농업과학원
  3. Allen MF, "The incidence and world distribution of the honey bee viruses", Bee World, 77, pp.141~162 (1996)[DOI]
  4. Benjeddou M. Leat N, "Detection of Acute Bee Paralysis Virus and Black Queen Cell Virus from honeybees by Reverse Transcriptase PCR", Appl Env Microbiol, 67, pp.2384~2387 (2001)[DOI]
  5. Core A, "A new threat to honey bees, the parasitic phorid fly Apocephalus borealis", PLoS One, 7(1), pp.e29639~ (2012)[DOI]
  6. Grabensteiner W, "Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using reverse trascription-PCR", Clin Diagn Lab Immunol, 8, pp.93~104 (2001)
  7. Ha JS, "A PCR detection method of Melissococcus pluton for rapid identification of European foulbrood", Korean J Apiculture, 20, pp.9~18 (2005)
  8. 전동민, "천안 ․ 아산지역 양봉농가 꿀벌질병 감염률 조사", 한국동물위생학회지, 36(2), pp.147~150 (2013), 한국동물위생학회
  9. 김혜경, "국내 꿀벌에서의 Sacbrood Virus (SBV) 진단", Journal of Apiculture, 23(2), pp.103~109 (2008), 한국양봉학회
  10. 김영주, "대전광역시 양봉농가의 꿀벌질병 감염률 조사", 한국동물위생학회지, 39(4), pp.253~258 (2016), 한국동물위생학회
  11. Kojima Y, "Infestation of Japanese native honey bees by tracheal mite and virus from non-native European honey bees in Japan", Microb Ecol, 62(4), pp.895~906 (2011)[DOI]
  12. 이혜민, "꿀벌 진균성 질병의 신속 확인을 위한 Ascosphera apis, Aspergillus favus의 PCR 검출법", Journal of Apiculture, 19(2) (2004), 한국양봉학회
  13. Lee HM, "Rapid identification of Ascosphera apis causing chalkbrood disease in honeybee by real-time PCR", Korean J Apiculture, 20, pp.109~116 (2005)
  14. Liu X, "Prevention of Chinese sacbrood virus infection in Apis cerana using RNA interference", Curr Microbiol, 61, pp.422~428 (2010)[DOI]
  15. 우인옥, "경북 동부지역 꿀벌에서 주요 병원체의 분자생물학적 검출", 한국동물위생학회지, 36(1), pp.37~44 (2013), 한국동물위생학회
  16. Ribiere M, "Molecular diagnostic of choronic bee paralysis virus infection", Apidologie, 33, pp.339~351 (2002)[DOI]
  17. Stoltz D, "Molecular diagnosis of Kashmir bee virus infection", J Api Res, 34, pp.153~160 (1995)[DOI]
  18. Thu HT, "Prevalence of bee viruses among Apis cerana populations in Vietnam", J Apic Res, 55, pp.379~385 (2016)[DOI]
  19. 유미선, "한국산 꿀벌에서 Black Queen Cell Virus의 검출", Journal of Apiculture, 22(1), pp.43~52 (2007), 한국양봉학회
  20. 유미선, "2009년 국내 꿀벌 질병의 발생 빈도", Journal of Apiculture, 24(4), pp.273~278 (2009), 한국양봉학회

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