Prevalence of feline calicivirus in Korean cats determined by an improved real-time RT-PCR assay
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Feline calicivirus (FCV) is considered the main viral pathogen of feline upper respiratory tract disease (URTD). The frequent mutations of field FCV strains result in the poor diagnostic sensitivity of previously developed molecular diagnostic assays. In this study, a more sensitive real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for broad detection of currently circulating FCVs and comparatively evaluated the diagnostic performance with previously developed qRT-PCR assay using clinical samples collected from Korean cat populations. The developed qRT-PCR assay specifically amplified the FCV p30 gene with a detection limit of below 10 copies/ reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 2%. Based on the clinical evaluation using 94 clinical samples obtained from URTD-suspected cats, the detection rate of FCV by the developed qRT-PCR assay was 47.9%, which was higher than that of the previous qRT-PCR assay (43.6%). The prevalence of FCV determined by the new qRT-PCR assay in this study was much higher than those of previous Korean studies determined by conventional RT-PCR assays. Due to the high sensitivity, specificity, and accuracy, the new qRT-PCR assay developed in this study will serve as a promising tool for etiological and epidemiological studies of FCV circulating in Korea. Furthermore, the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of FCV in Korea.
Abstract
Feline calicivirus (FCV) is considered the main viral pathogen of feline upper respiratory tract disease (URTD). The frequent mutations of field FCV strains result in the poor diagnostic sensitivity of previously developed molecular diagnostic assays. In this study, a more sensitive real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for broad detection of currently circulating FCVs and comparatively evaluated the diagnostic performance with previously developed qRT-PCR assay using clinical samples collected from Korean cat populations. The developed qRT-PCR assay specifically amplified the FCV p30 gene with a detection limit of below 10 copies/ reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 2%. Based on the clinical evaluation using 94 clinical samples obtained from URTD-suspected cats, the detection rate of FCV by the developed qRT-PCR assay was 47.9%, which was higher than that of the previous qRT-PCR assay (43.6%). The prevalence of FCV determined by the new qRT-PCR assay in this study was much higher than those of previous Korean studies determined by conventional RT-PCR assays. Due to the high sensitivity, specificity, and accuracy, the new qRT-PCR assay developed in this study will serve as a promising tool for etiological and epidemiological studies of FCV circulating in Korea. Furthermore, the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of FCV in Korea.
참고문헌 (45)
- Abd-Eldaim MM, "Development and validation of a TaqMan real-time reverse transcription-PCR for rapid detection of feline calicivirus", Arch Virol, 154, pp.555~560 (2009)
- Afonso MM, "A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity", Vaccine, 35, pp.2753~2760 (2017)
- Berger A, "Feline calicivirus and other respiratory pathogens in cats with feline calicivirus-related symptoms and in clinically healthy cats in Switzerland", BMC Vet Res, 11, pp.282~ (2015)
- Bustin SA, "The MIQE guidelines : minimum information for publication of quantitative real-time PCR experiments", Clin Chem, 55, pp.611~622 (2009)
- Cai Y, "An etiological investigation of domestic cats with conjunctivitis and upper respiratory tract disease in Japan", J Vet Med Sci, 64, pp.215~219 (2002)
- Cao L, "Isolation and phylogenetic analysis of feline calicivirus strains from various region of China", Anim Dis, 2, pp.16~ (2022)
- Cao N, "Development and application of a triplex TaqMan quantitative real-time PCR assay for simultaneous detection of feline calicivirus, feline parvovirus, and feline herpesvirus 1", Front Vet Sci, 8, pp.792322~ (2022)
- Chander Y, "A TaqManR RT-PCR assay for the detection of feline calicivirus", Int J Virol, 3, pp.100~106 (2007)
- Coyne KP, "Large-scale spatial and temporal genetic diversity of feline calicivirus", J Virol, 86, pp.11356~11367 (2012)
- Di Martino B, "Etiological investigation of multiple respiratory infections in cats", New Microbiol, 30, pp.455~461 (2007)
- Fernandez M, "Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydophila felis and Mycoplasma felis DNA and associated risk factors in cats in Spain with upper respiratory tract disease, conjunctivitis and", or gingivostomatitis, 2017, pp.19, 461~469
- Guo J, "Co-circulation and evolution of genogroups I and II of respiratory and enteric feline calicivirus isolates in cats", Transbound Emerg Dis, 69, pp.2924~2937 (2022)
- Helps CR, "Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats : experience from 218 European catteries", Vet Rec, 156, pp.669~673 (2005)
- Helps CR, "Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR", J Virol Methods, 106, pp.241~244 (2002)
- Henzel A, "Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil", Virus Res, 163, pp.667~671 (2012)
- Hofmann-Lehmann R, "Calicivirus Infection in Cats", Viruses, 14, pp.937~ (2022)
- Hou J, "European molecular epidemiology and strain diversity of feline calicivirus", Vet Rec, 178, pp.114~115 (2016)
- Jeon GT, "Tailored multiplex real-time RT-PCR with speciesspecific internal positive controls for detecting SARS-CoV-2 in canine and feline clinical samples", Animals, 13, pp.602~ (2023)
- Jeon GT, "An improved duplex real-time quantitative RT-PCR assay with a canine endogenous internal positive control for more sensitive and reliable detection of canine parainfluenza virus 5", Vet Sci, 10, pp.142~ (2023)
- Gyu-Tae Jeon, "A triplex real-time PCR assay for simultaneous and differential detection of Bordetella bronchiseptica, Mycoplasma cynos, and Mycoplasma canis in respiratory diseased dogs", 한국동물위생학회지, 46(1), pp.15~27 (2023), 한국동물위생학회[DOI]
- Johnson G, "Real-time quantitative PCR, pathogen detection and MIQE", Methods Mol Biol, 943, pp.1~16 (2013)
- Byeong-Teck Kang, "Prevalence of feline herpesvirus , feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter", Journal of Veterinary Science, 9(2), pp.207~209 (2008), 대한수의학회
- Hye-Ryung Kim, "Prevalence of Bordetella bronchiseptica, Mycoplasma felis, and Chlamydia felis using a newly developed triplex real-time polymerase chain reaction assay in Korean cat population", 한국동물위생학회지, 45(4), pp.305~316 (2022), 한국동물위생학회[DOI]
- Kim JM, "Molecular detection of porcine parainfluenza viruses 1 and 5 using a newly developed duplex real-time RT-PCR in South Korea", Animals(Basel), 13, pp.598~ (2023)
- Sung Jae Kim, "Development of a novel reverse transcription PCR and its application to field sample testing for feline calicivirus prevalence in healthy stray cats in Korea", Journal of Veterinary Science, 21(5), pp.0~0 (2020), 대한수의학회[DOI]
- Kwiecien R, "Concordance analysis : part 16 of a series on evaluation of scientific publications", Dtsch Arztebl Int, 108, pp.515~521 (2011)
- 이미진, "Prevalence study of respiratory pathogens in Korean cats using real-time polymerase chain reaction", 한국동물위생학회지, 45(3), pp.145~153 (2022), 한국동물위생학회[DOI]
- Lee SY, "Intergenic recombination in feline calicivirus associated with a hemorrhagic-like disease in the Republic of Korea", Acta Virol, 65, pp.232~236 (2021)
- Liu C, "Molecular and serological investigation of cat viral infectious diseases in China from 2016 to 2019", Transbound Emerg Dis, 67, pp.2329~2335 (2020)
- Meli ML, "Molecular detection of feline calicivirus in clinical samples : a study comparing its detection by RT-qPCR directly from swabs and after virus isolation", J Virol Methods, 251, pp.54~60 (2018)
- Michael HT, "Frequency of respiratory pathogens and SARS-CoV-2 in canine and feline samples submitted for respiratory testing in early 2020", J Small Anim Pract, 62, pp.336~342 (2021)
- Nguyen D, "Feline upper respiratory tract infection and disease in Australia", J Feline Med Surg, 21, pp.973~978 (2019)
- Palombieri A, "Detection and characterization of feline calicivirus associated with paw and mouth disease", Animals, 13, pp.65~ (2023)
- 박종인, "Virulent Systemic Feline Calicivirus Infection in a Kitten", 한국임상수의학회지, 32(5), pp.445~448 (2015), 한국임상수의학회
- Pesavento PA, "Molecular virology of feline calicivirus", Vet Clin North Am Small Anim Pract, 38, pp.775~786 (2008)
- Radford AD, "Feline calicivirus infection. ABCD guidelines on prevention and management", J Feline Med Surg, 11, pp.556~564 (2009)
- Radford AD, "The challenge for the next generation of feline calicivirus vaccines", Vet Microbiol, 117, pp.14~18 (2006)
- Radford AD, "The capsid gene of feline calicivirus contains linear B-cell epitopes in both variable and conserved regions", J Virol, 73, pp.8496~8502 (1999)
- Sato Y, "Phylogenetic analysis of field isolates of feline calicivirus(FCV)in Japan by sequencing part of its capsid gene", Vet Res Commun, 26, pp.205~119 (2002)
- Scansen BA, "Evaluation of a p30 gene-based real-time reverse transcriptase polymerase chain reaction assay for detection of feline caliciviruses", J Vet Intern Med, 18, pp.135~138 (2004)
- Spiri AM, "An update on feline calicivirus", Schweiz Arch Tierheilkd, 164, pp.225~241 (2022)
- Sykes JE, "Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia felis mucosal swabs by multiplex RT-PCR", PCR, 2001, pp.81, 95~108
- Vinjé J, "ICTV Virus Taxonomy Profile : Caliciviridae", J Gen Virol, 100, pp.1469~1470 (2019)
- Dong-Kun Yang, "Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea", 대한수의학회지, 60(4), pp.195~202 (2020), 대한수의학회[DOI]
- Zhou L, "Molecular characterization and cross-reactivity of feline calicivirus circulating in Southwestern China", Viruses, 13, pp.1812~ (2021)