염소의 피하농양 발생에 따른 혈액 내 염증지표 분석
Analysis of inflammatory markers in blood related with the occurrence of subcutaneous abscesses in goats
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초록
Subcutaneous abscesses, which occur mainly in goats and sheep, are lymph node abscesses caused by Corynebacterium pseudotuberculosis infection, and are divided into internal, external, and mixed types depending on the type of occurrence. While diagnostic methods for subcutaneous abscesses have been continuously studied, research reports for effective treatment and management of subcuta-neous abscesses are inadequate. Therefore, this study was conducted to determine the changes in bio-metric information related to the inflammatory markers of goats induced by subcutaneous abscesses by infection with C. pseudotuberculosis . For this, hematological tests, analysis of inflammatory indi-cators, and analysis of serum proteins through electrophoresis separation of goats with healthy goats and goats inoculated with C. pseudotuberculosis to induce subcutaneous abscesses were compared and analyzed by date, and the differences and characteristics were identified periodically. As a result, in goats induced with subcutaneous abscesses, anemia findings related to a rapid decrease in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) were observed, and a significant increase in inflammatory cells expressed in total white blood cell (WBC), neutrophil, and monocytes was observed. And the levels of acute phase protein (APP) such as fibrinogen, haptoglobin, and serum amyloid A (SAA) were observed to increase rapidly immediately after infection. In addition, in the results of electrophoretic analysis of serum proteins, it was observed that the levels of α-globulin and β-globulin were significantly increased in goats with subcutaneous abscesses. That is, when looking at these changes, it was found that the systemic inflammatory response of goats was rapidly induced immediately after infection with the C. pseudotuberculosis pathogen. Through this study, it was pos-sible to identify changes in the biomarkers of goats with subcutaneous abscesses, which had not been reported. Furthermore, these analyzed data are thoughts to be of great help in identifying, treating, and managing the goats of subcutaneous abscesses.
Abstract
Subcutaneous abscesses, which occur mainly in goats and sheep, are lymph node abscesses caused by Corynebacterium pseudotuberculosis infection, and are divided into internal, external, and mixed types depending on the type of occurrence. While diagnostic methods for subcutaneous abscesses have been continuously studied, research reports for effective treatment and management of subcuta-neous abscesses are inadequate. Therefore, this study was conducted to determine the changes in bio-metric information related to the inflammatory markers of goats induced by subcutaneous abscesses by infection with C. pseudotuberculosis . For this, hematological tests, analysis of inflammatory indi-cators, and analysis of serum proteins through electrophoresis separation of goats with healthy goats and goats inoculated with C. pseudotuberculosis to induce subcutaneous abscesses were compared and analyzed by date, and the differences and characteristics were identified periodically. As a result, in goats induced with subcutaneous abscesses, anemia findings related to a rapid decrease in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) were observed, and a significant increase in inflammatory cells expressed in total white blood cell (WBC), neutrophil, and monocytes was observed. And the levels of acute phase protein (APP) such as fibrinogen, haptoglobin, and serum amyloid A (SAA) were observed to increase rapidly immediately after infection. In addition, in the results of electrophoretic analysis of serum proteins, it was observed that the levels of α-globulin and β-globulin were significantly increased in goats with subcutaneous abscesses. That is, when looking at these changes, it was found that the systemic inflammatory response of goats was rapidly induced immediately after infection with the C. pseudotuberculosis pathogen. Through this study, it was pos-sible to identify changes in the biomarkers of goats with subcutaneous abscesses, which had not been reported. Furthermore, these analyzed data are thoughts to be of great help in identifying, treating, and managing the goats of subcutaneous abscesses.
참고문헌 (31)
- Abebe D, "Determination of Corynebacterium pseudotuberculosis prevalence and antimicrobial susceptibility pattern of isolates from lymph nodes of sheep and goats at an organic export abattoir, Modjo, Ethiopia", Lett Appl Microbiol, 61, pp.469~476 (2015)
- Al-Gaabary MH, "Caseous lymphadenitis in sheep and goats : Clinical, epidemiological and preventive studies", Small Rumin Res, 87, pp.116~121 (2009)
- Algammal A, "Molecular characterization and antibiotic susceptibility of Corynebacterium pseudotuberculosis isolated from sheep and goats suffering from caseous lymphadenitis", Zagazig Vet J, 44, pp.1~8 (2016)
- Baird G, "Current perspectives on caseous lymphadenitis", In Pract, 25, pp.62~68 (2003)
- Bastos BL, "Haptoglobin and fibrinogen concentrationsand leukocyte counts in the clinical investigation of caseous lymphadenitis in sheep", Vet Clin Pathol, 40, pp.496~503 (2011)
- Bastos BL, "Corynebacterium pseudotuberculosis : immunological responses in animal models and zoonotic potential", J Clin Cell Immunol S, 4, pp.005~ (2012)
- Brown C, "Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis", Am J Vet Res, 47, pp.1116~1119 (1986)
- Chirino-Zárraga C, "Bacteriological characterization of Corynebacterium pseudotuberculosis in Venezuelan goat flocks", Small Rumin Res, 65, pp.170~175 (2006)
- Choi SH, "A Study of Investigation the Actual conditions on Housing and Facilities for Korean Native Goat", Lives Hous & Env, 7, pp.13~20 (2001)
- Dorella FA, "Corynebacterium pseudotuberculosis : microbiology, biochemical properties, pathogenesis and molecular studies of virulence", Vet Res, 37, pp.201~218 (2006)
- Dorella FA, "Antigens of Corynebacterium pseudotuberculosis and prospects for vaccine development", Expert Rev Vaccines, 8, pp.205~213 (2009)
- Dorneles EM, "Evaluation of ERICPCR as genotyping method for Corynebacterium pseudotuberculosis isolates", PLoS One, 9, pp.e98758~ (2014)
- Fontaine M, "Caseous lymphadenitis", Small Rumin Res, 76, pp.42~48 (2008)
- Guerrero JAV, "Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico", Microb Pathog, 117, pp.304~309 (2018)
- Hoelzle LE, "Differences in the antigen structures of Corynebacterium pseudotuberculosis and the induced humoral immune response in sheep and goats", Vet Microbiol, 164, pp.359~365 (2013)
- Kaba J, "Development of an ELISA for the diagnosis of Corynebacterium pseudotuberculosis infections in goats", Vet Microbiol, 78, pp.155~163 (2001)
- Lee C-G, "Studies on The Diseases of the Korean Native Goat-A Review", J Vet Clin, 17, pp.32~44 (2000)
- Li H, "Isolation, antibiotic resistance, virulence traits and phylogenetic analysis ofCorynebacterium pseudotuberculosis from goats in southwestern China", Small Rumin Res, 168, pp.69~75 (2018)
- Literák I, "Phenotype and genotype characteristics of the Slovak and Czech Corynebacterium pseudotuberculosis strains isolated from sheep and goats", Small Rumin Res, 32, pp.107~111 (1999)
- Meyer R, "In vitro IFN-gamma production by goat blood cells afterstimulation with somatic and secreted Corynebacterium pseudotuberculosis antigens", Vet Immunol Immunopathol, 107, pp.249~254 (2005)
- Millar HR, "An evaluation of the heat precipitation method for plasma fibrinogen estimation", J Clin Pathol, 24, pp.827~830 (1971)
- Peel MM, "Human lymphadenitis due to Corynebacterium pseudotuberculosis : report of ten cases from Australia and review", J Clinical Infectious Diseases, 24, pp.185~191 (1997)
- Pépin M, "Experimental Corynebacterium pseudotuberculosis infection in lambs : kinetics of bacterial dissemination and inflammation", Vet Microbiol, 26, pp.381~392 (1991)
- Pépin M, "Caseous lymphadenitis in sheep and goats", Point Vet, 30, pp.33~40 (1999)
- Pinheiro Junior JW, "Corynebacterium pseudotuberculosis experimental infection of goats mamary gland", Arq Inst Biol, Sao Paulo, 73, pp.395~400 (2006)
- Piontkowski MD, "Evaluation of a commercially available vaccine against Corynebacterium pseudotuberculosis for use in sheep", J Am Vet Med Assoc, 212, pp.1765~1768 (1998)
- Ribeiro D, "Subclinical diagnosis of caseous lymphadenitis based on ELISA in sheep from Brazil", J Bacteriol, 4, pp.1~4 (2013)
- Schreuder BEC, "Corynebacterium pseudotuberculosis in milk of CL affected goats", Vet Rec, 127, pp.387~ (1990)
- Severini M, "Pseudotuberculosis in sheep as a concern of veterinary public health", Vet Res Commun, 27, pp.315~318 (2003)
- 손준형, "경북지역 재래산양의 세균성, 바이러스성 설사병 병원체 검출률 조사", 한국동물위생학회지, 39(1), pp.35~39 (2016), 한국동물위생학회
- Windsor PA, "Control of caseous lymphadenitis", Vet Clin North Am Food Anim Pract, 27, pp.193~202 (2011)