Bacillus subtilis K-54의 단백질 분해효소 처리에 의한 양모와 견의 품질개선효과
The Effect of Quality Improvement for Wool and Silk Treated with Protease Produced by B. subtilis K-54
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Abstract
For studies of fibrinolytic enzyme strain K-54 was isolated from the Korean traditional food chungkook-jang. Isolated strains K-54 was identified as Bacillus subtilis. The molecular weight of fibrinolytic enzyme from B. subtilis K-54 was 27 kDa. Optimum temperature for fibrinolytic enzyme of B. subtilis K-54 was 50-70oC and optimum pH for producing the enzyme of this strain was ranging from 8 to 12. Also, it was found out enzyme activity was completely inhibited by 1mM PMSF. The result indicated this enzyme was thermo-stable alkaline serine protease with strong fibrinolytic activity. The wool and silk were treated with protease of B. subtilis K-54. As a result, the property of dyeing of wool fabrics was increased. By the increasing of treatment time became smoothened. But the change of mechanical properties were not changed.
참고문헌 (16)
- 김노수, "반응성 염료에 의한 견날염에 관한 연구", 21(3), pp.134~143 (1984)
- 김노수, "견섬유의 물리적 구조변동의 균염성에 관한 연구", 19(3), pp.181~190 (1982)
- 김노수, "PH가 견 피브로인에 대한 모노클로로트리아진 반응 염료의 반응성에 미치는 영향", 16(1), pp.14~24 (1979)
- 김정희, "마직물의 셀룰라아제 효소처리에 의한 유연 가공효과에 관한 연구", 3(4), pp.367~372 (2001)
- 박병기, "생견의 비누정련과 효소정련에 대한 비교연구", 17(2), pp.94~99 (1977)
- 박병기, "섬유공학의 이해", pp.78~83 (2001), 서울: 시그마프레스
- 박병기, "I Reactive Orange 16에 의한 견직물염색", pp.11~21 (1980)
- 심현주, "구성사의 번수에 따른 면직물의 수세 및 효소가공 후의 역학적 성질의 변화", 39(3), pp.362~368 (2002)
- 유천권, "청국장에서 분리한 Bacillus subtilis K-54이 분리하는 혈전용해효소의 정제 및 특성", 26(6), pp.507~514 (1998)
- 윤남식, "양모섬유의 저온플라스마 및 효소처리", 6(4), pp.27~33 (1994)
- 이정숙, "단백질 분해 효소가 세척성에 미치는 영향", 17(3), pp.491~505 (1993)
- 홍기정, "셀룰라아제 처리에 의한 면의 개질 -인장 강도변화 및 염색성-", pp.277~285 (1994)
- Bruckner R, "Multiple active forms of a novel serine protease from B", pp.486~490 (1990)
- Bradford M, "A rapid and sensitive method for the quantitation of ug proteins", pp.248~251 (1976)
- Doe E, "Carboxypeptidase in cmmercial bromelain powder -Partial purification and some properties of pineapple carboxypeptidase-", pp.1063~1071 (1974)
- Kim S.J, "Use of protease produced by Bacillus sp SJ-121 for improvement of dyeing quality in wool and silk", 10(3), pp.186~191 (2005)