대장균과 살모넬라균에 대한 박하와 배초향 정유성분의 향균활성

Abstract

For developing natural presevatives, essential oils of Mentha arvensis L. var. piperascens Malivaud and Agastache urgosa O. Kuntze were analyzed the composition of two oils and experimented on microorgainsm, surival. Main components of Mentha arvensis iol were isomenthol (26.84%) and menthol (25.48%), and those of Agastache rugosa oil were estragole (79.83%) and limonene (4.13%) from GC-MSD analysis. Inhibition activities of Mentha arvensis oil were isomenthol (26.84%) and menthol (25.48%), and those of Agastache rugosa oil against growth of Escherichia coli O157 : H7 ATCC43895 and Salmonella typhimurium ATCC 7988 were observed from their clear Zone (9~14㎜ ＆ 9~13㎜), and that of Agastache rugosa oil were done from the clear zone (13~20㎜ ＆10~18㎜) by concentration-dependent manner, respectively. In the inhibition test on CFU/㎖ of the microorganisms, both of the plant essential oils at concentration of 5 and 10㎎ showed potent growth inhibition activities from 9 hour of incubation. Analysis using transmission electron microscope on E. coli also showed antimicrobial activities of the oils as deformation of the cell and loss of the intracellular materials.