초임계 추출 공법을 이용해 회향, 유향 및 노간주나무로부터 분리한 정유 성분의 생리활성 비교
Comparison of biological activities of essential oils from foeniculum vulgare Mill, boswellia carteii birew and juniperus rigida sieb. By a supercritical fluid extraction system
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Abstract
Essential oils from, Fennel fruit(Foeniculum vulgare Mill), Olibanum resin(Boswellia carteii Birew and Needl juniperrus stem(Juniperus rigida Sieb.) were extracted by a supercritical fluid extraction system(SFE) and biological activity of each essential oils were observed. SFE techinque was applied for the isolation and purification of nonpolar biologically active essential oils from each samples. The quantitative analysis of essential oils was carried out by gas chromatography-mass spectrometer(GC/MS). About 60% of the growth of AGS and A549 cells were inhibited by adding 1.0g/ℓ of the crude essential oils and below 40% was observed by the control. Cutotoxicity on huma 0nnormal lung cell(HEL299) was scored as 15~18% for the crude essential oils and 12% for control, respecitively. It meant that the essential oils were more effective than the control in anti-mutagenecity tested by CHO V79 cells. The effect of the essential oils on the growth of nerve cells. PC12 was observed as follows: The viable cell density was about two times higher than control.