가시오갈피 현탁배양 체세포배의 저온장기저장 및 식물체 재분화
Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim
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Abstract
A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at 4℃. The embryos resumed growth within two weeks when returned to MS liquid medium containing 0.2㎎/ℓ 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultrued at 32℃ in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeksof suspenmsion culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.