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Extraction Procedures for Free Radical Scavenging Activity from Noni Fruit (Morinda citrifolia)

홍정화|최희선|곽미경|Benya Manochai
한국약용작물학회|2011.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제19권 제1호|pp. 38-46

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참고문헌

키워드

DPPHQuercetinNoni (Morinda citrifolia)Chlorogenic AcidScopoletin

초록

In this study, we investigated maximum yield of radical scavenging activity (RSA) using each of various extraction solvents and procedure from noni friut (Morinda citrifolia). Noni fruits were either sun-dried (FS) or hot-air dried (FO) at 60℃ after steam blanching. For optimum aqueous extraction, noni fruits should be sun dried and autoclave extraction time should not be over 30 min to produce extraction with high RSA with low cost. In case of 50% ethanol extraction,reflux extracts of FS and FO resulted in IC_50 of 1.92 ㎎/mL and 3.06 ㎎/mL at 8 hr. When IC_50 values were lower than 5 ㎎/mL, coefficient of correlation was 0.71 indicating that 71% of the phenolic antioxidants in noni fruits were accounted for the activity by scavenging free DPPH. However, coefficient of correlation significantly decreased to 0.63 over IC_50 values of 5 ㎎/mL. Autoclave extraction contained chlorogenic acid of 14.69 ㎍/mL and scopoletin of 3.86 ㎍/mL. Reflux extraction showed all three compounds, chlorogenic acid (26.19 ㎍/mL), quercetin (19.59 ㎍/mL), and scopoletin (17.4 ㎍/mL). Therefore,the result of this study indicated that the potential antioxidant activities and functional values were obtained significantly with reflux extraction from noni fruit.

Abstract

In this study, we investigated maximum yield of radical scavenging activity (RSA) using each of various extraction solvents and procedure from noni friut (Morinda citrifolia). Noni fruits were either sun-dried (FS) or hot-air dried (FO) at 60℃ after steam blanching. For optimum aqueous extraction, noni fruits should be sun dried and autoclave extraction time should not be over 30 min to produce extraction with high RSA with low cost. In case of 50% ethanol extraction,reflux extracts of FS and FO resulted in IC_50 of 1.92 ㎎/mL and 3.06 ㎎/mL at 8 hr. When IC_50 values were lower than 5 ㎎/mL, coefficient of correlation was 0.71 indicating that 71% of the phenolic antioxidants in noni fruits were accounted for the activity by scavenging free DPPH. However, coefficient of correlation significantly decreased to 0.63 over IC_50 values of 5 ㎎/mL. Autoclave extraction contained chlorogenic acid of 14.69 ㎍/mL and scopoletin of 3.86 ㎍/mL. Reflux extraction showed all three compounds, chlorogenic acid (26.19 ㎍/mL), quercetin (19.59 ㎍/mL), and scopoletin (17.4 ㎍/mL). Therefore,the result of this study indicated that the potential antioxidant activities and functional values were obtained significantly with reflux extraction from noni fruit.

참고문헌 (48)

  1. Adam M, "Extraction of antioxidants from plants using ultrasonic methods and their antioxidant capacity", Journal of Separation Science, 32, pp.288~294 (2009)
  2. 부희옥, "여주 품종별 폴리페놀, 플라보노이드 함량과 라디칼 소거활성 및 아질산염 소거능", 한국약용작물학회지, 17(1), pp.15~20 (2009), 한국약용작물학회
  3. Brand-Willian W, "Use of a free radical method to evaluate antioxidant activity", Lebensmittel- Wissenschaft und Technologia, 28, pp.25~30 (1995)
  4. Calzuola I, "Comparative activity of antioxidants from wheat sprouts, Morinda citrifolia, fermented papaya and white tea", International Journal of Food Science and Nutrition, 57, pp.168~177 (2006)
  5. Cardon D, "Le Monde des Teintures Naturelles", pp.273~352 (2003), Belin
  6. Dittmar A, "Morinda citrifolia L. use in indigenous Samoan medicine", Journal of Herbs, Spices, and Medicine Plants, 1, pp.77~92 (1993)
  7. Dixon AR, "this: the transformation of Noni, a traditional Polynesian medicine (Morinda citrifolia, Rubiaceae)", Ecological Botany, 53, pp.51~68 (1999)
  8. Fraga CG, "Oxidative damage to DNA during aging: 8-hydroxy-2’- deoxyguanosine in rat organ DNA and urine", Proceedings of the National Academy of Sciences, 87, pp.4533~4537 (1990)
  9. Furusawa E, "Antitumor potential of a polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) on sarcoma 180 ascites tumour in mice", Phytotherapy Research, 17, pp.1158~1164 (2003)
  10. Hemwimon S, "crowaveassisted extraction of antioxidative anthraquinones from roots of Morinda citrifolia", Separation and Purification Technology, 54, pp.44~50 (2007)
  11. Hirazumi A, "An immunomodulatory polysaccharide- rich substance from the fruit juice of Morinda citrifolia (noni) with antitumour activity", Phytotherapy Research, 13, pp.380~387 (1999)
  12. Hornick CA, "Inhibition of angiogenic initiation and disruption of newly established human vascular networks by juice from Morinda citrifolia (noni)", Angiogenesis, 6, pp.143~149 (2003)
  13. 장상훈, "수확시기별, 부위별 선학초의 폴리페놀함량 및 DPPH 라디컬 소거능의 변화", 한국약용작물학회지, 16(6), pp.397~401 (2008), 한국약용작물학회
  14. 정향숙, "복분자 추출물의 나노입자화를 통한 항산화 및 미백 효과 증진", 한국약용작물학회지, 17(2), pp.81~89 (2009), 한국약용작물학회
  15. Kalia K, "Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguineaLodd. and quantification of its phenolic constituents by RP-HPLC", Journal of Agricultural and Food Chemistry, 56, pp.10129~10134 (2008)
  16. Kamiya K, "Chemical constituents of Morinda citrifolia fruits inhibit copperinduced low-density lipoprotein oxidation", Journal of Agricultural and Food Chemistry, 52, pp.5843~5848 (2004)
  17. Kayano S, "Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist", Journal of Agricultural and Food Chemistry, 50, pp.3708~3712 (2002)
  18. Li H, "Solvent effects on focused microwave assisted extraction of polyphenolic acids from Eucommia ulmodies", Phytochemcal Analysis, 15, pp.306~312 (2004)
  19. Liu GM, "Two novel glycosides from the fruits of Morinda citrifolia (noni) inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line", Cancer Research, 61, pp.5749~5756 (2001)
  20. Madhujith T, "Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties", Journal of Agricultural and Food Chemistry, 5421, pp.8048~8057 (2006)
  21. Maillard MN, "Evolution of antioxidant activity during kilning: role of insoluble bound phenolic acids of barley and malt", Journal of Agricultural and Food Chemistry, 43, pp.1789~1793 (1995)
  22. Mohd Zin, "Antioxidative activity of extracts from Mengkudu (Morinda citrifolia L.) root, fruit, and leaf", Food Chemistry, 78, pp.227~231 (2002)
  23. Morton JF, "The ocean-going Noni, or Indian mulberry (Morinda citrifolia, Rubiaceae) and some of its ‘‘colourful’’ relatives", Ecological Botany, 46, pp.241~256 (1992)
  24. Namiki M, "Antioxidants", antimutagens in foods, 1990, pp.29, 273~280
  25. Nelson SC, "Noni cultivation in Hawaii", Fruit and Nuts, 4, pp.1~4 (2001)
  26. Ollanketo M, "Extraction of sage (Salvia officialis L.) by pressurized hot water and conventional methods: antioxidant activity of the extracts", European Food Research and Technology, 215, pp.158~163 (2002)
  27. 박원종, "구기자 품종과 교배종의 부위별 심혈관관련 질환 예방 기능성 및 항산화 활성비교", 한국약용작물학회지, 15(6), pp.391~397 (2007), 한국약용작물학회
  28. Pia L, "Storage stability of microencapsulated cloudberry (Rubus chamaemorus) phenolics", Journal of Agricultural and Food Chemistry, 56, pp.11251~11261 (2008)
  29. Pongnaravane B, "Extraction of anthraquinones from roots of Morindaby pressurized hot water: Antioxidant activity of extracts", Journal of Supercritical Fluids, 37, pp.390~396 (2006)
  30. Ross IA, "Medical Plants of the World. Chemical Constituents, Traditional and Modern Medical Uses", pp.351~394 (2001), Humana Press
  31. Saludes JP, "Antitubercular constituents from the hexane fraction of Morinda citrifolia Linn. (Rubiaceae)", Phytotherapy Research, 16, pp.683~685 (2002)
  32. Sanchez-Moreno C, "A procedure to measure the antiradical efficiency of polyphenols", Journal of the Science of Food and Agriculture, 76, pp.270~276 (1998)
  33. Sang S, "Flavonol glycoside and novel iridoid glycoside from the leaves of Morinda citrifolia", Journal of Agricultural and Food Chemistry, 49, pp.4478~4481 (2001)
  34. Sang S, "New unusual iridoids from the leaves of Noni (Morinda citrifolia L.) show inhibitory effect on ultraviolet B-induced transcriptional activator protein-1 (AP-1) activity", Bioorganic and Medicinal Chemistry, 11, pp.2499~2502 (2003)
  35. Sharma UK, "Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC)", Journal of Agricultural and Food Chemistry, 56, pp.374~379 (2008)
  36. Shotipruk A, "Pressurized hot water extraction of anthraquinones from the roots of Morinda citrifolia", Biotechnology Progress, 20, pp.1872~1875 (2004)
  37. Simic MG, "Mechanism of inhibition of free-radical processes in mutagenesis and carcinogenesis", Mutation Research, 202, pp.377~386 (1988)
  38. Singleton V, "Analysis of total phenols and other oxidation substances and antioxidants by means of Folin-ciocalteu reagent", Methods in Enzymology, 299, pp.152~175 (1999)
  39. Su BN, "Chemical constituents of the fruits of Morinda citrifolia (noni) and their antioxidant activity", Journal of Natural Products, 68, pp.592~595 (2005)
  40. Tan XJ, "Simultaneous determination of 13 bioactive compounds in Herba artemisiae Scopariae (Yin Chen) from different harvest seasons by HPLC-DAD", Journal of Parmaceutical and Biomedical Analysis, 47, pp.847~853 (2008)
  41. Wang M, "Novel trisaccharide fatty acid ester identified from the fruits of Morinda citrifolia (noni)", Journal of Agricultural and Food Chemistry, 47, pp.4880~4882 (1999)
  42. Wang MF, "Novel glycosides from noni (Morinda citrifolia)", Journal of Natural Products, 63, pp.1182~1183 (2000)
  43. Wang MY, "Morinda citrifolia (Noni): a literature review and recent advances in Noni research", Acta Pharmacological Sinica, 23, pp.1127~1141 (2002)
  44. Whistler WA, "Traditional and herbal medicine in the Cook Islands", Journal of Ethnopharmacology, 13, pp.239~80 (1985)
  45. Yamaguchi S, "of angiotensin I converting enzyme by noni (Morinda citrifolia) juice", Journal of the Japanese Society for Food Science and Technology, 49, pp.624~627 (2002)
  46. Yang J, "Free-radical-scavenging activity and total phenols of noni (Morinda ctrifolia) juice and powder in processing and storage", Food Chemistry, 102, pp.302~308 (2007)
  47. Zhu X, "Optimization of microwave-assisted solvent extraction for volatile organic acids in tobacco and its comparison with conventional extraction methods", Analytica Chimica Acta, 579, pp.88~94 (2006)
  48. Zin ZM, "Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.)", Food Chemistry, 94, pp.169~178 (2006)

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