추출용매와 부위에 따른 고추 추출물의 세포독성
Cytotoxicity of Extracts from Korean Pepper (Capsicum annuum L.) by Extraction Solvents and Plant Parts
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In order to find out anticancer activity of Korean pepper (Capsicum annuum L.), the cytotoxicity against 8cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) of extracts by extraction solvents andplant parts were investigated using MTT assay. Also the correlation between content of capsaicin known as anticanceringredient and cytotoxicity of extracts from pepper were analyzed. The distilled water extracts from seed and germinatedseed showed very high cytotoxicity against 6 cancer cell lines including A549 (lung carcinoma cells), AGS (stomach adenocarcinomacells), HeLa (cervix adenocarcinoma cells), HepG2 (hepatoblastoma cells), HT-29 (colon adenocarcinoma cells),and MCF-7 (breast adenocarcinoma cells). But 80% ethanol and methanol extracts showed cytotoxicity against 293 andAGS. The RC50, that was, the concentration of sample required for 50% reduction of cell viability, of seed and germinatedseed extracts against AGS were 33.4 ~ 389.1㎍/㎖ and 63.9 ~ 1,316.7㎍/㎖, respectively, so anticancer activity was higher inseed than in germinated seed. In capsaicin contents, seed with high cytotoxicity and pericarp with a little cytotoxicity contained47.4 ~ 1,260.0㎍/g and 58.3 ~ 1,498.0㎍/g, respectively. As these results, the correlation was not between cytotoxicityand capsaicin content.
Abstract
In order to find out anticancer activity of Korean pepper (Capsicum annuum L.), the cytotoxicity against 8cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) of extracts by extraction solvents andplant parts were investigated using MTT assay. Also the correlation between content of capsaicin known as anticanceringredient and cytotoxicity of extracts from pepper were analyzed. The distilled water extracts from seed and germinatedseed showed very high cytotoxicity against 6 cancer cell lines including A549 (lung carcinoma cells), AGS (stomach adenocarcinomacells), HeLa (cervix adenocarcinoma cells), HepG2 (hepatoblastoma cells), HT-29 (colon adenocarcinoma cells),and MCF-7 (breast adenocarcinoma cells). But 80% ethanol and methanol extracts showed cytotoxicity against 293 andAGS. The RC50, that was, the concentration of sample required for 50% reduction of cell viability, of seed and germinatedseed extracts against AGS were 33.4 ~ 389.1㎍/㎖ and 63.9 ~ 1,316.7㎍/㎖, respectively, so anticancer activity was higher inseed than in germinated seed. In capsaicin contents, seed with high cytotoxicity and pericarp with a little cytotoxicity contained47.4 ~ 1,260.0㎍/g and 58.3 ~ 1,498.0㎍/g, respectively. As these results, the correlation was not between cytotoxicityand capsaicin content.
참고문헌 (32)
- Ana GC, "Determination of capsaicin and dihydrocapsaicin in Capsicum fruits by liquid chromatograhy-electrospray", time-offlight mass spectrometry, 2006, pp.54, 9303~9311
- Erin N, "Capsaicin-induced inactivation of sensory neurons promotes a more aggressive gene expression phenotype in breast cancer cells", Breastcancer Research and Treatment, 99, pp.351~364 (2006)
- Galvez AF, "A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells", Nature Biotechnology, 17, pp.495~500 (1999)
- 강현민, "고추와 Capsaicin의 항산화 효능 비교", Journal of Pharmacopuncture, 11(1), pp.109~118 (2008), 대한약침학회
- Huang SP, "Capsaicin-induced apoptosis in human hepatma HepG2 cells", Anticancer Research, 29, pp.165~174 (2009)
- 황은주, "발아 메밀 추출물의 항산화·항균활성 및 세포독성", 한국약용작물학회지, 14(1), pp.1~7 (2006), 한국약용작물학회
- 전방실, "Orotic Acid-유발 지방간에 미치는 Capsaicin의 개선효과", 한국식품영양과학회지, 33(5), pp.815~820 (2004), 한국식품영양과학회
- 정미리, "육성계통에 따른 고추의 생리활성 평가", 한국식품영양과학회지, 40(5), pp.642~648 (2011), 한국식품영양과학회
- 김규열, "인간 대장암 세포주에서 Capsaicin에 의한 세포고사에 관한 연구", 대한외과학회지, 62(2), pp.103~111 (2002), 대한외과학회
- Kim SH, "Analysis of chemical constituents in fruits of red pepper(Capsicum annuum L. cv. Bugang)", Journal of the Korean Society for Horticultural Science, 38, pp.384~390 (1997)
- Kun-Young Park, "Fermented Wheat Grain Products and Kochujang Inhibit the Growth of AGS Human Gastric Adenocarcinoma Cells", Preventive Nutrition and Food Science, 10(4), pp.349~352 (2005), 한국식품영양과학회
- 김선영, "종양세포에서의 capsaicin에 의한 apoptosis 유도와항암제의 항암효과의 증가", Korean Journal of Pediatrics, 51(3), pp.307~314 (2008), 대한소아과학회
- 구경형, "고추씨의 물과 에탄올 추출물의 생리활성", 한국식품영양과학회지, 37(10), pp.1357~1362 (2008), 한국식품영양과학회
- 구강모, "고춧잎 품종별 항산화 활성과 항산화 성분", Journal of Applied Biological Chemistry, 52(2), pp.70~76 (2009), 한국응용생명화학회
- 권기수, "생육시기 및 부위별 고추의 항산화력 및 항암 Lunasin peptide의 동정", 생명과학회지, 15(4), pp.528~535 (2005), 한국생명과학회
- 권민철, "강화 사자발쑥의 항암활성 증진을 위한 추출조건의 최적화", 한국약용작물학회지, 15(4), pp.233~240 (2007), 한국약용작물학회
- Lee HD, "Relationships between the taste components and sensory preference of Korean red peppers", Korean Journal of Food Science and Technology, 24, pp.266~271 (1992)
- Lee JS, "Capsaicininduced apoptosis and reduced release of reactive oxygen species in MBT-2 murine bladder tumor cells", Archives Pharmacal Research, 27, pp.1147~1153 (2004)
- 이성응, "국내 재배지역에 따른 고추의 Capsaicinoid 함량", 한국식품영양과학회지, 42(1), pp.129~133 (2013), 한국식품영양과학회
- Lee SW., "Physio-chemical studies on the after-ripening of hot pepper fruits(part 6)Hot-taste component in different parts and of capsaicin homologues", Journal of Korean Society of Agricultural Chemistry and Biotechnology, 14, pp.157~163 (1971)
- Lo YC, "Capsaicin-induced cell death in a human gastric adenocarcinoma cell line", World Journal of Gastroenterology, 11, pp.6254~6257 (2005)
- Ministry of Agriculture, Food and Rural Affairs(MAFRA)., "The cultural situation and actual output of greenhouse vegetables in 2013", pp.11~43 (2014), Ministry of Agriculture, Food and Rural Affairs
- Oyagbemi AA, "Capsaicin : A novel chemopreventive molecule and its underlying molecular mechanisms of action", Indian Journal of Cancer, 47, pp.53~58 (2014)
- 서윤정, "흰쥐 모델에서 Capsaicin이 소장 콜레스테롤 및 중성지방 흡수율에 미치는 영향", 한국식품영양과학회지, 38(12), pp.1712~1717 (2009), 한국식품영양과학회
- ㄴTsou MF, "Involvement of Bax, Bcl-2, Ca2+and caspase-3 in capsaicin-induced apoptosis of human leukemia HL-60 cells", Anticancer Research, 26, pp.1965~1971 (2006)
- Wang HM, "Downregulation of tumor-associated NADH oxidase, tNOX(ENOX2), enhances capsaicin-induced inhibition of gastric cancer cell growth", Cell Biochemistry and Biophysics, 61, pp.355~366 (2011)
- Wilkinson F, "Isolation and identification of a protein with capsaicin-inhibited NADP oxidase activity from culture media conditioned by growth of HeLa cells", Archives of Biochemistry and Biophysics, 336, pp.275~282 (1996)
- Yang KM, "Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines", Cellular and Molecular Biology Letters, 14, pp.497~510 (2009)
- 윤효진, "품종별 청고추의 항산화 효과 및 유방암 세포주에서의 세포 사멸 연구", 한국식품과학회지, 44(6), pp.750~758 (2012), 한국식품과학회[DOI]
- Yoon HS, "Studies on the development of food resources from waste seeds. IV. Chemical composition of red pepper seed", Journal of the Korean Society of Food Science and Nutrition, 12, pp.46~50 (1983)
- 윤형권, "고추 과실의 성숙에 따른 매운맛 및 색소 관련 성분의 변화", Horticulture, Environment, and Biotechnology, 43(1), pp.39~42 (2002), 한국원예학회
- Zhang R, "In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway", Apoptosis, 13, pp.1465~1478 (2008)