아로니아 저온 초음파 추출물의 항비만 활성 증진
Enhancement of Anti-Obesity Activities of Aronia melanocarpa Elliot Extracts from Low Temperature Ultrasonification Process
2
조회
0
다운로드
14
인용
0
참고문헌
키워드
초록
Background: This study represents the first report that the anti-obesity activity of ethanol extracts of Aronia melanocarpa can be enhanced through ultrasonification at a frequency of 120 ㎑ at 60℃ (UE). Methods and Results: The amounts of cyanidin-3-O-galactose (cya-gal), a major anthocyanin in A. melanocarpa were higher by up to 402.4 ㎎/100 g, as compared with 221.4 ㎎/100 g and 322.1 ㎎/100 g, for hot water at 100℃ and 70% ethanol at 80℃ respectively. This result should cause the higher antioxidant activities of the UE than extract of hot water and ethanol in DPPH free radical scavenging. It was confirmed that the high antioxidant activity of UE could play an important role in inhibiting the production of proteins related to adipocyte differentiation, such as peroxisome proliferator activated receptor-γ (PPAR-γ) and sterol regulatory element binding protein 1 (SREBP1). Conclusions: Ultrasonification at a frequency of 120 ㎑ at 60℃ should result in better anti-obesity activity than that observed using other processes. It was also observed for the first time that the anti-obesity activity of A. melanocarpa was associated with its antioxidant activity, possibly due to the higher elution of intact cya-gal, owing to efficient low temperature ultrasonification extraction. These results could also be applied to improve other biological activities of medicinal herbs that contain many types of heat-labile bioactive substances.
Abstract
Background: This study represents the first report that the anti-obesity activity of ethanol extracts of Aronia melanocarpa can be enhanced through ultrasonification at a frequency of 120 ㎑ at 60℃ (UE). Methods and Results: The amounts of cyanidin-3-O-galactose (cya-gal), a major anthocyanin in A. melanocarpa were higher by up to 402.4 ㎎/100 g, as compared with 221.4 ㎎/100 g and 322.1 ㎎/100 g, for hot water at 100℃ and 70% ethanol at 80℃ respectively. This result should cause the higher antioxidant activities of the UE than extract of hot water and ethanol in DPPH free radical scavenging. It was confirmed that the high antioxidant activity of UE could play an important role in inhibiting the production of proteins related to adipocyte differentiation, such as peroxisome proliferator activated receptor-γ (PPAR-γ) and sterol regulatory element binding protein 1 (SREBP1). Conclusions: Ultrasonification at a frequency of 120 ㎑ at 60℃ should result in better anti-obesity activity than that observed using other processes. It was also observed for the first time that the anti-obesity activity of A. melanocarpa was associated with its antioxidant activity, possibly due to the higher elution of intact cya-gal, owing to efficient low temperature ultrasonification extraction. These results could also be applied to improve other biological activities of medicinal herbs that contain many types of heat-labile bioactive substances.
참고문헌 (32)
- Attie AD, "Adipocyte metabolism and obesity", The Journal of Lipid Research, 50, pp.395~399 (2009)
- Barstard JP, "Recent advances in the relationship between obesity, inflammation and insulin resistance", European Cytokine Network, 17, pp.4~12 (2006)
- Brunlich M, "Extracts, anthocyanins and procyanidins from Aronia melanocarpa as radical scavengers and enzyme inhibitors", Nutrients, 5, pp.663~678 (2013)
- Camp HS, "Adipogenesis and fat-cell function in obesity and diabetes", Trends in Molecular Medicine, 8, pp.442~447 (2002)
- 최혜민, "오적산 (五積散)의 고지방식이 마우스 지방축적억제 및 3T3-L1지방세포에서의 비만 조절 기전에 관한 연구", 대한본초학회지, 30(4), pp.121~128 (2015), 대한본초학회
- Chung KW, "Ultrasonic energy effects on squalene extraction from amaranth seed", Applied Chemistry, 4, pp.149~152 (2000)
- d’Alessandro LG, "Ultrasound assisted extraction of polyphenols from black chokeberry", Separation and Purification Technology, 93, pp.42~47 (2012)
- Dietz BM, "Xanthohumol isolated from Humulus lupulus inhibits menadione-induced DNA damage through induction of quinone reductase", Chemical Research in Toxicology, 18, pp.1296~1305 (2005)
- Horton JD, "SREBPs:Transcriptional mediators of lipid homeostasis", Cold Spring Harbor Symposia on Quantitative Biology, 67, pp.491~498 (2002)
- 허은주, "적색 국화의 화색발현에 있어 고온에 민감한 생육 단계와 품종별 고온적응성 비교", 원예과학기술지, 26(4), pp.357~361 (2008), 한국원예학회
- Hwang YP, "Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphateactivated protein kinase in human HepG2 cells and obese mice", Nutrition Research, 31, pp.896~906 (2011)
- Jayaprakasam B, "Amelioration of obesity and glucose intolerance in high-fat-fed C57BL", 6 mice by anthocyanins and ursolic acid in Cornelian cherry(Cornus mas), 2006, pp.54, 243~248
- Kao ES, "Anthocyanin extracted from Hibiscus attenuate oxidized LDLmediated foam cell formation involving regulation of CD36gene", Chemico-Biological Interactions, 179, pp.212~218 (2009)
- Kim HG, "Influence of roasting on the antioxidant activity of small black soybean(Glycine max L. Merrill).", Food Science and Technology, 44, pp.992~998 (2011)
- 김재범, "지방세포 분화와 지방세포 분비물질들의 기능", Endocrinology and Metabolism, 17(1), pp.1~9 (2002), 대한내분비학회
- 김남영, "추출방법에 따른 아로니아 추출물의 주름 개선 효능 비교", 한국약용작물학회지, 22(3), pp.217~222 (2014), 한국약용작물학회[DOI]
- 이미경, "가지 물추출물의 3T3-L1 지방전구세포 분화 억제효능", 약 학 회 지, 55(4), pp.309~313 (2011), 대한약학회
- 이옥환, "Production of Reactive Oxygen Species and Changes in Antioxidant Enzyme Activities during Differentiation of 3T3-L1 Adipocyte", Applied Biological Chemistry, 52(1), pp.70~75 (2009), 한국응용생명화학회
- 이화, "베리류 에탄올 추출물의 항산화 활성", 한국약용작물학회지, 23(1), pp.49~56 (2015), 한국약용작물학회[DOI]
- Lowell BB, "Towards a molecular understanding of adaptive thermogenesis", Nature, 404, pp.652~660 (2000)
- Ohgami K, "Anti-inflammatory effect of aronia extract on rat endotoxininduced uveitis", Investigative Ophthalmology and Visual Science, 46, pp.275~281 (2005)
- Oszmiaski J, "Aronia melanocarpa phenolics and their antioxidant activity", European Food Research and Technology, 221, pp.809~813 (2005)
- 박성진, "소리쟁이 분획물의 지방세포 분화 억제 효과", 한국식품과학회지, 45(1), pp.90~96 (2013), 한국식품과학회[DOI]
- Rayalam S, "Phytochemicals and regulation of the adipocyte life cycle", The Journal of Nutritional Biochemistry, 19, pp.717~726 (2008)
- Rosen ED, "Adipocytes as regulators of energy balance and glucose homeostasis", Nature, 444, pp.847~853 (2006)
- Slimestad R, "Flavonoids from black chokeberries, Aronia melanocarpa", Journal of Food Composition and Analysis, 18, pp.61~68 (2005)
- Spielgelman BM, "The adipocyte : A multifunctional cell", Cell Metabolism, 4, pp.425~427 (2006)
- Tanaka T, "Chemical components and characteristics of black chokeberry", Journal of the Japanese Society for Food Science and Technology, 48, pp.606~610 (2001)
- Valko M, "Free radicals and antioxidants in normal physiological functions and human disease", The International Journal of Biochemistry and Cell Biology, 39, pp.44~84 (2007)
- Wang H, "Oxygen radical absorbing capacity of anthocyanins", Journal of Agricultural and Food Chemistry, 45, pp.304~309 (1997)
- 유재상, "고지방식이 비만마우스 모델에서 파키스탄산 및 중국산 마황으로 조성된 降脂丸-1, 2, 3, 4와 降脂丸-1合加味消滯丸의 체중감량효과 비교", 한방비만학회지, 15(1), pp.9~23 (2015), 한방비만학회
- 유순집, "비만의 약물 치료", Endocrinology and Metabolism, 23(4), pp.223~233 (2008), 대한내분비학회