저온처리 온도와 상대습도가 2년생 인삼의 동해에 미치는 영향
Effects of Cold Temperature and Relative Humidity on the Freezing Stress of Two Year-old Ginseng Seedlings
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Background: Abrupt cold, especially frost after seedling emergence, in spring causes servere losses in ginseng yield as it is a perennial crop and has a determinate growth. Although abnormal temperature occurs more frequently, the physiological aspects of ginseng to cold stress are poorly understood. Here we report the freezing response of ginseng under artificial conditions. Methods and Results: Cold treatment at -4℃ for 16 h resulted in a 21.4% damage rate in a cold chamber, however, no damage was observed at -2℃ and -4℃ for 2, 4, 6, or 8 h. High humidity increased the damage rate to 59.9% even at the higher temperature (RH 95%, -3℃) than the ambient temperature (RH 66%, -4℃) of the cold chamber. Seedlings early in the leaf expansion were damaged more than older ones. The quantum yield (Fv/Fm) of cold-damaged seedlings was lower than the control or seedlings without visual symptoms. The root weight of a damaged seedling one month after cold treatment was 58% of control. Conclusions: Our results suggested that visual symptoms are correlated with photosynthetic ability and yield after freezing stress in ginseng. We expect our results to contribute to the further understanding of ginseng freezing stress.
Abstract
Background: Abrupt cold, especially frost after seedling emergence, in spring causes servere losses in ginseng yield as it is a perennial crop and has a determinate growth. Although abnormal temperature occurs more frequently, the physiological aspects of ginseng to cold stress are poorly understood. Here we report the freezing response of ginseng under artificial conditions. Methods and Results: Cold treatment at -4℃ for 16 h resulted in a 21.4% damage rate in a cold chamber, however, no damage was observed at -2℃ and -4℃ for 2, 4, 6, or 8 h. High humidity increased the damage rate to 59.9% even at the higher temperature (RH 95%, -3℃) than the ambient temperature (RH 66%, -4℃) of the cold chamber. Seedlings early in the leaf expansion were damaged more than older ones. The quantum yield (Fv/Fm) of cold-damaged seedlings was lower than the control or seedlings without visual symptoms. The root weight of a damaged seedling one month after cold treatment was 58% of control. Conclusions: Our results suggested that visual symptoms are correlated with photosynthetic ability and yield after freezing stress in ginseng. We expect our results to contribute to the further understanding of ginseng freezing stress.
참고문헌 (30)
- Anderson KA, "Phenological development of different Vitis(grape)cultivars(Relation to freeze damage)", Fruit Varieties Journal, 34, pp.5~7 (1980)
- Andrews PK, "Deep supercooling of dormant and deacclimating Vitis buds", American Journal of Enology and Viticulture, 35, pp.175~177 (1984)
- Arias NS, "Freezing avoidance by supercooling in Olea europaea cultivars: The role of apoplastic water, solute content and cell wall rigidity", Plant, Cell and Environment, 38, pp.2061~2070 (2015)
- "Freeze protection methods for crops" (1985)[URL]
- Cameron RWF, "Air temperature, humidity and rooting volume affecting freezing injury to Rhododendron and other perennials", Journal of Horticultural Science, 72, pp.553~562 (1997)
- Cary JW, "Factors influencing freezing of supercooled water in tender plants", Agronomy Journal, 62, pp.715~719 (1970)
- Jing Chen, "Structural variation, functional differentiation and expression characteristics of the AP2", ERF gene family and its response to cold stress and methyl jasmonate in Panax ginseng C.A. Meyer, 2020, pp.15(3), e0226055~[DOI]
- Devi BSR, "Influence of potassium nitrate on antioxidant level and secondary metabolite genes under cold stress in Panax ginseng", Russian Journal of Plant Physiology, 59, pp.328~325 (2012)
- Fuller MP, "A chamber for the simulation of radiation freezing of plants", Annals of Applied Biology, 133, pp.111~121 (1998)
- Fuller MP, "An investigation of the frost hardiness of grapevine(Vitis vinifera)during bud break", Annals of Applied Biology, 135, pp.589~595 (1999)
- Hopkins WG, "Introduction to Plant Physiology", pp.467~476 (2003), John Wiley and Sons Inc
- Jeong HN, "Early budding characteristics and freezing damage of 5-Year-Old Ginseng(Panax ginseng C. A. Meyer)by different shading material in regional unusual temperature", Proceedings of Journal of Medicinal Crop Science, 11, pp.137~138 (2012)
- Johnson DE, "Factors influencing critical temperatures for spring freeze damage to developing primary shoots on Concord grapevines", American Journal of Enology and Viticulture, 32, pp.144~149 (1981)
- Keller M, "The science of grapevines: Anatomy and physiology", pp.274~286 (2010), Academic Press
- Kim JU, "Growth characteristics of aerial parts of 2-year-old cultivar of Korean ginseng, Yunpoong, based on climate change scenarios in Jeju", Proceedings of Journal of Medicinal Crop Science, 22, pp.94~95 (2014)
- Kratsch HA, "The ultrastructure of chilling stress", Plant, Cell and Environment, 23, pp.337~350 (2000)
- 국용인, "저온스트레스와 회복기간중 저온 내성 및 감수성 벼 품종의 생리적 반응", 한국환경농학회지, 20(2), pp.93~98 (2001), 한국환경농학회
- Lee JC, "Low temperature tolerance of Panax quinquefolium", Korean Journal of Ginseng Sicence, 20, pp.179~183 (1996)
- Lee JH, "Isolation and characterization of a Glutamate decarboxylase(GAD)gene and their differential expression in response to abiotic stresses from Panax ginseng C. A. Meyer", Molecular Biology Reports, 37, pp.3455~3463 (2010)
- Qian Liu, "Identification, characterization and functional differentiation of the NAC gene family and its roles in response to cold stress in ginseng, Panax ginseng C.A. Meyer", PLOS ONE, 15(6), pp.e0234423~ (2020), Public Library of Science (PLoS)[DOI]
- Lu YZ, "Artificial radiation frost chamber for frost formation on tea", Applied Science, 9, pp.4726~ (2019)
- Luisetti J, "Pseudomonas syringae pv. syringae as one of the factors affecting the ice nucleation of grapevine buds in controlled conditions", Journal of Phytopathology, 133, pp.334~344 (1991)
- 오순자, "저온스트레스 지표 선발을 위한 아열대성 식물 4종의 O-J-I-P곡선 분석", 한국환경과학회지, 14(4), pp.389~395 (2005), 한국환경과학회
- Park H, "Physiological disorder of Panax ginseng", Korean Journal of Crop Science, 36, pp.459~480 (1991)
- Pearce RS, "Plant freezing and damage", Annals of Botany, 87, pp.417~424 (2001)
- Peng Y, "Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants", Planta, 226, pp.729~740 (2007)
- Rizza F, "Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats", Plant Breeding, 120, pp.389~396 (2001)
- White book for the damage of ginseng by the abnormal climate: Low temperature, high temperature, wind, flooding, and snow, "Rural Development Administration(RDA)", Rural Development Administration, 8~31 (2018)
- Wisniewski M, "Use of a hydrophobic particle film as a barrier to extrinsic ice nucleation in tomato plants", Journal of American Society for Horticultural Science, 127, pp.358~364 (2002)
- Zhang R, "Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment", Plant Physiology and Biochemistry, 71, pp.203~211 (2013)