생약추출물이 Aspergillus parasiticus의 Aflatoxin B1 생성에 미치는 영향

Abstract

The influences of the extracts from Cinnamomi cortex, Eucommiae Cortex, Puerariae Radix, Lycii Fructus, Zixyphi Fructus, Schisandrae Fructus, Mume Fructus, Chaenomelis Fructus on mycelial growth and aflatoxin B1 production from Aspergillus parasiticus were analyzed. The pH of the culture media were redeced to below pH 4 by all the herbal extracts after 3 days incubation. However, the pH of the culture media increased above pH 6 after 6 days incubation using the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix and Lycii Fructus. The mycelial growth of A. parasiticus was increased over the amount of the control. Puerariae Radix produced the largest amount of mycelial growth and Chaenomelis Fructus produced the smallest amount of mycelialgrowth. The productions of aflatoxin B1 from A. parasticus culture were increased by the extracts of Puerariae Radix and Zizyphi Fructus, while inhibited by the extractso of Cinnamomi Cortes, Eucommiae Cortex, Lycii Fructus, Schisandrae fructus, Mume Fructus and Chaenomelis Fructus. In particular, the extracts of Cinnamomi Cortex, Lycii Fructus and schisandrae Fructus almost inhibited the preduction of aflatoxin b1. The production of the total protein from Cinnamomi Cortex, which produced much less aflatoxin B1, and Puerariae Radix, whichproduced a great deal of aflatoxin B1 from A. parasticus were slightly higher than the production of the total protein of the control medium.