Extracts from Pinus densiflora Siebold et Zuccarini Sawdust Inhibit the Mycelial Growth of Lentinus edodes (Berk.) Sing., Edible Mushroom

Abstract

The extracts of Pinus densiflora sawdust by n hexane, ethyl acetate and methanol solvent were investigated to identify their myce]ial growth inhibition against Lentinus edodes. The yields of n hexane soluble fraction, ethyl acetate-soluble fraction, and methanol soluble fraction from P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvent, respectively. The mycelial growth inhibition of L, edodes was the greatest for n hexane extract, ranging from 36.5% to 47.6% at concentrations of 125 ppm to 1,000 ppm, with the values for all concentrations significantly different from one another. After direct extraction of P. densiflora sawdust using n hexane, ethyl acetate and methanol, each extract was separate into three fractions by silica gel column chromatography and then the fractions were isolated on the values Rf by thin layer chromatography. The mycelial growth inhibition against L, edodes was recognized in the fractions Ⅱ (33.5%) and Ⅲ (37.6%) of n hexane extract, the fraction Ⅱ(21.4%) of ethyl acetate extract and the fraction Ⅱ(26.4%) of methanol extract. The fractions Ⅲ of n-hexane extract showed the highest growth inhibition among the nine factions of the organic solvent extract.