Agrobacterium을 이용한 Phosphinothricin Acetyl Transferase의 도라지로의 형질전환
Agrobacterium-mediated Transformation of PAT into Platycodon grandiflorum A. De. Candolle
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
This study was conducted to introduce phosphinothricin acetyl transferase (PAT) gene, resistant to basta which was non-selective herbicide, into balloon flower (Platycodon grandiflorum A. De. candolle). Seeds were germinated on MS medium, and 10-day-old immature cotyledon explants and 30-day-old leaf explants were cocultured with Agrobacterium tumefaciens strain MP 90 (pBinSyn) on 1/10 MS medium for 48 hours in the dark at 25℃. The cultures were transferred for selection of kanamycin-resistant shoots to the MS medium supplemented with 0.2 ㎎/ℓ NAA, 1.0 ㎎/ℓ BA, 3% sucrose, 100 ㎎/ℓ kanamycin, 500 ㎎/ℓ carbenicillin. Shoots were obtained from 10-day-old immature cotyledon explants after 4 weeks of culture. The shoots were subcultured twice every 4 weeks on the same medium for growth of transgenic shoots. Successful transformation was confirmed by histochemical GUS assay, PCR analysis, RT-PCR analysis, 10 ㎎/ℓ phosphinothricin treatment and 0.3% basta spray. The basta-resistant transgenic plants flowered normally.
참고문헌 (18)
- "Agrobacterium-mediated genetic transformation of a phalaenopsis orchid", 19, pp.435~442 (2000)
- "Molecular and cytogenetic analysis of transgenic plants of rice (Oryza sativa L.) produced by Agrobacterium-mediated transformation", 7(1), pp.39~46 (2004)
- "Expression and inheritance of bar gene in Petunia hybrida transformed with Agrobacterium", 30(2), pp.143~149 (2003)
- "Production of transgenic grapevine (Vitis vinifera L.) plants by co-cultivation of embryogenic calli with Agrobacterium tumefaciens and selecting secondary embryos", 15(1), pp.29~33 (1998)
- "Assaying chimeric genes in plants", pp.387~ (1987)
- "Seed treatment procedure to promote seedling emergence of Platycodon grandiflorum", 10(2), pp.75~81 (2002)
- "Adventitious bud induction and plant regeneration from cotyledon explants of Camellia japonica L", 13(3), pp.105~108 (2005)
- "Factors affecting somatic embryogenesis from immature cotyledon of soybean", 6(1), pp.45~50 (2004)
- "Genotypes of high competencefor somatic embryogenesis and plant regeneration in soybeanGlycine max. Theor. Appl. Genet. 75", pp.695~700 (1988)
- "Studieson a new antibiotic SF-1293. Isolation and physico-chemicaland biological characterization of SF-1293 substance. Sci. Rep.Meiji Seika Kaisha 13", pp.34~41 (1973)
- "Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants", 12(3), pp.191~202 (2004)
- "A comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum by HPLC analysis", 65, pp.428~431 (1999)
- "Effect of split application of nitrogen fertilizer on growth and yield in Platycodon grandiflorum A. DC", 12(6), pp.437~441 (2004)
- "Action mechanism of bialaphos. . Accumulation of ammonia inplants treated with bialaphos. J. Pest. Sci. 11", pp.33~37 (1986)
- "Agrobacterium-mediated transformation of a doubled haploid line of cabbage", 21, pp.257~262 (2002)
- "A cassette containing thebar gene of Streptomyces hygroscopicus", pp.1062~1065 (1990)
- "Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber", 28(1), pp.53~58 (2001)
- "Genetic transformation of Panax ginseng with herbicide resistant gene", 28(6), pp.353~357 (2001)