In-vitro Anti-inflammatory Activity of Rubus coreanusMiq. on Nitric Oxide, Interferon-γ, Cycloxygenase-2, andTumor Necrosis Factor-α Production in the Macrophagelike Cell Line RAW 264.7
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To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the efectsRubus coreanus Miq. (ERC) on lipolysaccharide-induced nitric oxide (NO), tumor necrosis factor-α(TNF-α), and Interferon-γ (IFN-γ) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is apotent inhibitor of NO production and it also significantly decreased IFN-γ and TNF-α production. Consistent with theseresults, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanolefects posibly by supresing the inducible NO synthase and COX-2 expressions.
Abstract
To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the efectsRubus coreanus Miq. (ERC) on lipolysaccharide-induced nitric oxide (NO), tumor necrosis factor-α(TNF-α), and Interferon-γ (IFN-γ) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is apotent inhibitor of NO production and it also significantly decreased IFN-γ and TNF-α production. Consistent with theseresults, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanolefects posibly by supresing the inducible NO synthase and COX-2 expressions.
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