제주 손바닥 선인장의 초음파 추출을 통한 면역활성 증진
Enhancement of Immune Activities of Opuntia ficus-indica L. Miller by Ultrasonification Extraction Process
2
조회
0
다운로드
13
인용
0
참고문헌
키워드
초록
Immune activities of Opuntia ficus indica L. Miller were investigated through aqueous extracts associated with ultrasonification process at 60℃, and showed the highest promotion of human B and T cell growth, up to 52% and 41%, respectively, compared to the control. The secretion of TNF-α and IL-60 was also enhanced by the addition (0.5 ㎎/㎖) of the extracts, up to 48%. NK cell activation was significantly improved up to 1.3 times higher than the case of adding other extracts. It was also found that extracts from O. ficus indica could yield higher nitric oxide production from macrophage than Lipopolysaccaharides (LPS). It can be concluded that, in general, the extracts treated with ultrasonification has higher immune acitivities than others, possibly by higher yielding immuno-modulatory than conventional extraction process. The optimum condition for the extraction of O. ficus-indica is water extraction associated with ultrasonification.
Abstract
Immune activities of Opuntia ficus indica L. Miller were investigated through aqueous extracts associated with ultrasonification process at 60℃, and showed the highest promotion of human B and T cell growth, up to 52% and 41%, respectively, compared to the control. The secretion of TNF-α and IL-60 was also enhanced by the addition (0.5 ㎎/㎖) of the extracts, up to 48%. NK cell activation was significantly improved up to 1.3 times higher than the case of adding other extracts. It was also found that extracts from O. ficus indica could yield higher nitric oxide production from macrophage than Lipopolysaccaharides (LPS). It can be concluded that, in general, the extracts treated with ultrasonification has higher immune acitivities than others, possibly by higher yielding immuno-modulatory than conventional extraction process. The optimum condition for the extraction of O. ficus-indica is water extraction associated with ultrasonification.
참고문헌 (27)
- Ahn DK, "Illustrated book of Korean medicinal herb", pp.497~497 (1998), Kyohaksa. Seoul.
- Barna BP, "Macrophage activation and generation of tumoricidal activity by liposome-associated human C-reactive protein", Cancer Res, 44(1), pp.305~310 (1984)
- Cecilia K, "IL-2 receptor signaling through the Shb adapter protein in T and NK cells", Biochemical and Biophysical Research Communications, 296, pp.929~936 (2002)
- Chung K, "Ultrasonic energy effects on squalene extraction from amaranth seed", Applied Chemistry, 4(2), pp.149~152 (2000)
- Chung MS, "Stability of betanine extracted from Opuntia ficus-indica var. saboten", Korean J. Soc. Food Sci, 12(4), pp.506~510 (1996)
- Cunnane G, "Mechanism of joint destruction in rheumatoid arthritis", Arch. Immunol. Ther. Exp.(Warsx), 46, pp.1~7 (1998)
- Ding AH, "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages", J. Immunol, 141, pp.2407~2412 (1998)
- Gay S, "Molecular and cellular mechanisms of joint destruction in rheumatoid arthritis : Two cellular mechanisms explain joint destruction", Ann. Rheum Dis, 52, pp.39~47 (1993)
- Han BH, "Effect of ginsenosides from Panax ginseng on TNF-α production and T cell proliferation", Yakhak Hoeji, 42(3), pp.296~301 (1998)
- Hotta M, "Useful plants of the World", pp.53~53 (1989), Pyungbumsa. Tokyo.
- 이현용, "저온 추출 공정에 의한 마황과 복분자의 면역 활성 증진 효과", 한국약용작물학회지, 13(3), pp.81~86 (2005), 한국약용작물학회
- Kim IH, "Effect of antioxidants on the thermostabillity of red pigment in prickly pear", Korean J. Food Sci. Technol, 27(6), pp.1013~1016 (1995)
- 이현용, "족제비싸리 부위별 추출물의 항암 및 면역활성", 한국약용작물학회지, 13(1), pp.41~47 (2005), 한국약용작물학회
- Kim TJ, "Korean source of plants II", pp.266~266 (1996), Seoul National University Publishing Dept., Seoul.
- Kim WI, "Ultrasound energy effects on solvent extraction of amaranth seed oil", J. Korean Ind. Eng. Chem, 12(3), pp.307~311 (2001)
- Lee HJ, "study on antiulcer effects of Opuntia dillenii Haw. on stomach ulcer induced by waterimmersion stress in wats", 석사, pp.1997
- Lee MK, "Enhancement immune activity and cytotoxicity of Artemisia capillaris Thrunb. extracts against human cell lines", Korean J. Med. Crop Sci, 12(1), pp.34~42 (2004)
- 이미경, "해당화 뿌리 추출물의 면역 증진 효과", 한국약용작물학회지, 11(1), pp.13~18 (2003), 한국약용작물학회
- 이서호, "황칠나무 잎의 면역활성증진기능 탐색", 한국약용작물학회지, 10(2), pp.109~115 (2002), 한국약용작물학회
- Lee YC, "Compositions of Opuntia ficus-indica", Korean J. Food Sci. Technol, 29(5), pp.847~853 (1997)
- Leistad L, "Detection of cytokine mRNA in human. articular cartilage from patients with rheumatoid arthritis and osteoarthritis by reverse transcriptasepolymerase chain reaction", Scand J. Rheumatoil, 27, pp.61~67 (1998)
- Miyagawa N, "Effect of c-reactive protein on peritoneal macrophages. II. Human C-reactive protein activates peritoneal macrophages of guinea pigs to release superoxide anion in vitro", Microbiol Immunol, 32(7), pp.721~731 (1988)
- 이현용, "팔손이 용매별 추출물의 항암 및 면역 활성 탐색", 한국약용작물학회지, 13(3), pp.87~92 (2005), 한국약용작물학회
- 박진홍, "초음파 병행추출을 이용한 가시오갈피, 마황, 복분자 및 인진쑥의 항암활성 증진", 한국약용작물학회지, 12(4), pp.273~278 (2004), 한국약용작물학회
- Pyungbumsa, "Useful plants of the World", pp.53~53 (1989), Tokyo
- Tanuma N, "Competitive PCR quantification of proand anti-inflammatory cytokine mRNA in the central nervous system during autoimmune encephalomyelitis", J. neuroimmunol, 73, pp.197~206 (1997)
- Yueran Z, "Expression of leptin receptors and response to leptin stimulation of human natural killer cell lines", Biochemical and Biophysical Research Communications, 300, pp.247~252 (2003)