Free Radical Scavenging Activity and Protective Effect against H_2O_2-Induced Stress in Neuronal Cells of Enzymatic Extracts from Sarcodon aspratus
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참고문헌
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초록
The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast,Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (α-chymotrypsin, Alcalase, Flavourzyme,Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration (IC_50) values were 0.896 and 0.734㎎/mL,respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their IC_50 values were 0.278 and 0.575㎎/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against H_2O_2-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.
Abstract
The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast,Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (α-chymotrypsin, Alcalase, Flavourzyme,Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration (IC_50) values were 0.896 and 0.734㎎/mL,respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their IC_50 values were 0.278 and 0.575㎎/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against H_2O_2-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.
참고문헌 (34)
- Bobek P, "Cholesterol-lowering effect of the mushroom Pleurotus ostreatus in hereditary hypercholesterolemic rats", Annals of Nutrition and Metabolism, 35, pp.191~195 (1991)
- Bobek P, "Oyster mushroom (Pleurotus ostreatus) decreases serum and liver cholesterol and increases cholesterol 7á-hydroxylase activity and fecal excretion of neutral sterols and bile acids in hypercholesterolemic rats", Nutrition Research, 14, pp.1683~1688 (1994)
- Bobek P, "Mechanism of hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats: reduction of cholesterol absorption and increase of plasma cholesterol removal", Zeitschrift für Ernährungswissenschaft, 33, pp.44~50 (1994)
- Bobek P, "Hypocholesterolemic and antiatherogenic effect of oyster mushroom (Pleurotus ostreatus) in rabbits", Nahrung, 43, pp.339~342 (1999)
- Boveris A, "Cellular sources and steadyc state levels of reactive oxygen species, In Oxygen, Gene Expression, and Cellular Function", pp.1~25 (1997), Marcel Dekker
- Cashman JR, "Monoamine oxidase and flavin-containing monooxygenase, In Comprehensive Toxicology", pp.69~96 (1997), Pergamon
- Cheung IM, "Antioxidant activity and total phenolics of edible mushroom extracts", Food Chemistry, 81, pp.249~55 (2003)
- Cumby N, "Antioxidant activity and water-holding capacity of canola protein hydrolysates", Food Chemistry, 109, pp.144~148 (2008)
- Guillamon E, "Edible mushrooms: Role in the prevention of cardiovascular diseases", Fitoterapia, 81, pp.715~723 (2010)
- Fischer LR, "Oxidative stress induced by loss of Cu,Zn-superoxide dismutase (SOD1) or superoxidegenerating herbicides causes axonal degeneration in mouse DRG cultures", Acta Neuropathol, 119, pp.249~259 (2010)
- Fridovich I, "Superoxide radical and superoxide dismutases", Annual Review of Biochemistry, 64, pp.97~112 (1995)
- Halliwell B, "Antioxidant defence mechanisms: from the beginning to the end (of the beginning)", Free Radical Research Communication, 31, pp.261~272 (1999)
- Hiramoto K, "DNA breaking activity of the carbon-centered radical generated from 2, 2-azobis(2-amidinopropane)-hydrochloride (AAPH)", Free Radical Research Communication, 19, pp.323~332 (1993)
- Je JY, "Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis", PROCESS BIOCHEMISTRY, 42(5), pp.840~846 (2007), ELSEVIER SCI LTD
- 정용준, "상황버섯과 발아현미상황버섯 열수추출물의 Murin Macrophage RAW 264.7 세포에서 항염증 반응 비교", 한국약용작물학회지, 17(2), pp.97~101 (2009), 한국약용작물학회
- Jose N, "Methanol extract of the oyster mushroom, Pleurotus florida, inhibits inflammation and platelet aggregation", Phytotherapy Research, 18, pp.43~46 (2004)
- Kabir Y, "Effect of shitake (Lentinus edodes) and Maitake (Grifola frondosa) mushrooms on blood pressure and plasma lipids of spontaneously hypertensive rats", Japanese Society of Nutrition and Food Science and Vitaminology, 33, pp.341~346 (1987)
- Kabir Y, "Dietary effect of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypertensive rats (SHR)", Japanese Society of Nutrition and Food Science and Vitaminology, 34, pp.433~438 (1988)
- Kabir Y, "Dietary mushrooms reduce blood pressure in spontaneously hypertensive rats", Journal of Nutritional Science and Vitaminology, 35, pp.91~94 (1989)
- Kalonia H, "Neuroprotective effect of MK-801 against intra-striatal quinolinic acid induced behavioral, oxidative stress and cellular alterations in rats", Indian Journal of Experimental Biology, 47, pp.880~892 (2009)
- Kaneda T, "Effect of various mushroom preparations on cholesterol levels in rats", Journal of Nutrition, 90, pp.371~376 (1966)
- Kohno K, "Anti-inflammatory and immunomodulatory properties of 2-amino-3H-phenoxazin-3-one", Biological and Pharmaceutical Bulletin, 31, pp.1938~1945 (2008)
- Senug Eun Lee, "Effects of Medicinal Plant Extracts on Antioxidant System in Ethanol-intoxicated Rats", 한국약용작물학회지, 12(2), pp.108~112 (2004), 한국약용작물학회
- 이승은, "식물자원의 항산화활성 탐색", 한국약용작물학회지, 16(5), pp.356~370 (2008), 한국약용작물학회
- Seung-Jae Lee, "Neuroprotective Effect of Hericium erinaceum against Oxidative Stress on PC12 Cells", Journal of the Korean Society for Applied Biological Chemistry, 53(3), pp.283~289 (2010), 한국응용생명화학회
- Miyazawa N, "Antihypertensive effect of Pleurotus nebrodensis in spontaneously hypertensive rats", Journal of Oleo Science, 57, pp.675~681 (2008)
- Nanjo F, "Scavenging effects of tea catechins and their derivatives on 1, 1-diphenyl-2-picrylhydrazyl radical", Free Radical Biology and Medicine, 21, pp.890~895 (1996)
- Nakajima Y, "Antioxidant small phenolic ingredients in inonotus obliquus (persoon) pilat (Chaga)", Chemical & Pharmaceutical Bulletin, 55, pp.1222~1226 (2007)
- Park PJ, "Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii", Journal of Agricultural and Food Chemistry, 53, pp.6666~6672 (2005)
- Reubsaet FA, "cyl-CoA oxidase activity and peroxisomal fatty acid oxidation in rat tissues", Biochimica et Biophysica Acta, 958, pp.434~442 (1988)
- Sheu SS, "Targeting antioxidants to mitochondria: A new therapeutic direction", Biochimica et Biophysica Acta, 1762, pp.256~265 (2006)
- Taylor RE, "Protein immobilization. fundamentals and applications", pp.2~ (1991), Marcel Dekker
- Wong JY, "Antioxidant properties of selected tropical wild edible mushrooms", Journal of Food Composition and Analysis, 22, pp.269~277 (2009)
- 신승렬, "능이버섯(Sarcodon aspratus) 추출물의 항산화성과 항균성", 한국식품영양과학회지, 35(8), pp.967~972 (2006), 한국식품영양과학회