지치 육묘시 발생하는 유묘썩음증상 원인균 분리·동정 및 경감 육묘환경에 관한 연구
Identification of Fungal Pathogen Causing Seedling Rot of Lithospermum erythrorhizon and Study on the Optimum Growing Temperature for Decreasing of the Seedling Rot
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This study was conducted to isolate and identify the fungal pathogen causing seedling rot of Lithospermum erythrorhizon Siebold & Zuccarini, and to know the optimum growing temperature for decreasing seedling rot of Lithospermum erythrorhizon. On the basis of morphological characteristics, EF-1a sequence analysis, and pathogenecity to host plant,the fungi isolated from seedling rot and seeds of Lithospermum erythrorhizon were identified as Fusarium fujikuroi, indicating that disease causing fungus is seed-borne pathogen. Optimum temperature for germination of seeds of Lithospermum erythrorhizon was 15 ~ 20℃, but pathogenicity of Fusarium fujikuroi was shown more readily at 25 ~ 30℃. These results suggested that seedling culture of Lithospermum erythrorhizon between 15℃ and 20℃ might reduce seedling rot of Lithospermum erythrorhizon caused by seed-borne pathogen Fusarium fujikuroi.
Abstract
This study was conducted to isolate and identify the fungal pathogen causing seedling rot of Lithospermum erythrorhizon Siebold & Zuccarini, and to know the optimum growing temperature for decreasing seedling rot of Lithospermum erythrorhizon. On the basis of morphological characteristics, EF-1a sequence analysis, and pathogenecity to host plant,the fungi isolated from seedling rot and seeds of Lithospermum erythrorhizon were identified as Fusarium fujikuroi, indicating that disease causing fungus is seed-borne pathogen. Optimum temperature for germination of seeds of Lithospermum erythrorhizon was 15 ~ 20℃, but pathogenicity of Fusarium fujikuroi was shown more readily at 25 ~ 30℃. These results suggested that seedling culture of Lithospermum erythrorhizon between 15℃ and 20℃ might reduce seedling rot of Lithospermum erythrorhizon caused by seed-borne pathogen Fusarium fujikuroi.
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