H2O2로 유도된 C6 신경교세포의 세포사멸에 대한 감초 추출물과 감초 활성물질의 보호효과
Protective Effects of Glycyrrhiza uralensis Radix Extract and Its Active Compounds on H2O2-induced Apoptosis of C6 Glial Cells
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Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer’s disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on H2O2-induced oxidative damage in C6 glial cells. Methods and Results: We exposed C6 glial cells to hydrogen peroxide (H2O2) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by H2O2 in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression. Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.
Abstract
Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer’s disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on H2O2-induced oxidative damage in C6 glial cells. Methods and Results: We exposed C6 glial cells to hydrogen peroxide (H2O2) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by H2O2 in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression. Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.
참고문헌 (34)
- Ahn J, "Protective effects of Glycyrrhiza uralensis Fisch. on the cognitive deficits caused by β-amyloid peptide 25-35 in young mice", Biogerontology, 7, pp.239~247 (2006)
- Asl MN, "Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds", Phytotherapy Research, 22, pp.709~724 (2008)
- Behl C, "Hydrogen peroxide mediates amyloid beta protein toxicity", Cell, 77, pp.817~827 (1994)
- de Bernardo S, "Role of extracellular signal-regulated protein kinase in neuronal cell death induced by glutathione depletion in neuron", glia mesencephalic cultures, 2004, pp.91, 667~682
- Desagher S, "Astrocytes protect neurons from hydrogen peroxide toxicity", The Journal of Neuroscience, 16, pp.2553~2562 (1996)
- Finkel T, "Oxidants, oxidative stress and the biology of ageing", Nature, 408, pp.239~247 (2000)[DOI]
- Giacobini E, "Cholinesterase inhibitors stabilize Alzheimer’s disease", Annals of the New York Academy of Sciences, 920, pp.321~327 (2000)
- Gong H, "A protective mechanism of licorice(Glycyrrhiza uralensis) : Isoliquiritigenin stimulates detoxification system via Nrf2 activation", Journal of Ethnopharmacology, 162, pp.134~139 (2015)
- 한샛별, "원산지별 감초 추출물의 항산화 활성 비교", 대한화장품학회지, 39(1), pp.1~8 (2013), 대한화장품학회
- Hardy J, "The amyloid hypothesis of Alzheimer's disease : Progress and problems on the road to therapeutics", Science, 297, pp.353~356 (2002)
- Hwang CK, "Isoliquiritigenin isolated from licorice Glycyrrhiza uralensis prevents 6-hydroxydopamineinduced apoptosis in dopaminergic neurons", Bioscience Biotechnology and Biochemistry, 76, pp.536~543 (2012)
- Jang JH, "Protective effects of resveratrol on hydrogen peroxide-induced apoptosis in rat pheochromocytoma(PC12)cells", Mutation Research, 496, pp.181~190 (2001)
- Jung JC, "Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease", BMC Complementary and Alternative Medicine, 16, pp.1~10 (2015)
- Kao TC, "Neuroprotective effects of glycyrrhizic acid and 18β-glycyrrhetinic acid in PC12 cells via modulation of the PI3K", Akt pathway, 2009, pp.57, 754~761
- 이홍규, "Inhibitory Effects of Glycyrrhizae Radix and Its Active Component, Isoliquiritigenin, on Aβ(25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons", Archives of Pharmacal Research, 35(5), pp.897~904 (2012), 대한약학회
- Lim GP, "The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse", The Journal of Neuroscience, 21, pp.8370~8377 (2001)
- Marchesi E, "Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein : A spin trapping and direct electron spin resonance study with implications for oxidative stress measurements", Free Radical Biology and Medicine, 26, pp.148~161 (1999)
- Mosmann, T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, pp.55~63 (1983)
- Oda A, "Oxidative stress upregulates presenilin 1 in lipid rafts in neuronal cells", Journal of Neuroscience Research, 88, pp.1137~1145 (2010)
- Oltvai ZN, "Bcl-2heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death", Cell, 74, pp.609~619 (1993)
- Oyama Y, "Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide", Brain Research, 712, pp.349~352 (1996)
- 박지해, "감초(Glycyrrhiza uralensis Fisch.)로부터 분리된 flavonoid의 인체 암세포에 대한 세포독성", Journal of Applied Biological Chemistry, 54(1), pp.67~70 (2011), 한국응용생명화학회
- Qian Y, "GRP78 compartmentalized redistribution in Pb-treated glia : Role of GRP78 in lead-induced oxidative stress", Neuro Toxicology, 26, pp.267~275 (2005)
- Sen CK, "Oxygen toxicity and antioxidants : State of the art", Indian Journal of Physiology and Pharmacology, 39, pp.177~196 (1995)
- Smith MA, "Oxidative stress in Alzheimer’s disease", Biochimica et Biophysica Acta, 1502, pp.139~144 (2000)
- Teng L, "Involvement of the ERK pathway in the protective effects of glycyrrhizic acid against the MPP+-induced apoptosis of dopaminergic neuronal cells", International Journal of Molecular Medicine, 34, pp.742~748 (2014)
- 엄민영, "생약추출물의 acetylcholinesterase 저해, 항산화 및 신경세포보호 효과in vitro 탐색", 한국식품저장유통학회지, 20(6), pp.840~845 (2013), 한국식품저장유통학회
- 엄영란, "HPLC를 이용한 발효 감초의 지표 성분 분석", 한국한의학연구원논문집, 15(1), pp.85~89 (2009), 한국한의학연구원
- Vila M, "Targeting programmed cell death in neurodegenerative diseases", Natures Reviews Neuroscience, 4, pp.365~375 (2003)
- Wang D, "ERKs and mitochondria-related pathways are essential for glycyrrhizic acidmediated neuroprotection against glutamate-induced toxicity in differentiated PC12 cells", Brazilian Journal of Medical and Biological Research, 47, pp.773~779 (2014)
- Warner HR, "Superoxide dismutase, aging, and degenerative disease", Free Radical Biology and Medicine, 17, pp.249~258 (1994)
- Whittemore ER, "A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture", Neuroscience, 67, pp.921~932 (1995)
- Yang EJ, "Isoliquiritigenin isolated from Glycyrrhiza uralensis protects neuronal cells against glutamateinduced mitochondrial dysfunction", Biochemical and Biophysical Research Communications, 421, pp.658~664 (2012)
- Yang EJ, "Neuroprotective effects of liquiritigenin isolated from licorice roots on glutamateinduced apoptosis in hippocampal neuronal cells", Neuro Toxicology, 39, pp.114~123 (2013)