Isolation of Guaian-6,12-olides from Artemisia princeps and Their Antioxidant Activity
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Background: Korean mugwort, Artemisia princeps, a plant that has been widely used in diet and traditional medicine throughout East Asia. Methods and Results: Two novel guaian-6,12-olides (1 and 2) and two known compounds (3 and 4) were isolated from Korean mugwort, their chemical structures were established by extensive spec- troscopic analysis and comparison with those of known compounds. The antioxidant capacity of the isolated compounds was evaluated by measuring intracellular reactive oxygen species (ROS) genera- tion in HT-1080 cells and the extent of oxidative damage to genomic DNA purified from HT-1080 cells. Compounds 1 and 3 exhibited outstanding intracellular ROS scavenging activity at a concentra- tion of 10 µM compared to the untreated blank. Particularly, 3 exhibited an exceptional ROS scaveng- ing activity of approximately 67% at a concentration of 1 µM after 2 h. Additionally, all compounds significantly inhibited DNA oxidation induced by hydroxyl radicals at a concentration greater than 1 µM. Among them, 4 showed the most effective protection against DNA damage. Conclusions: Further studies are expected to provide more insight on development of guaian-6,12- olides as potential antioxidants and mechanism by which these types of guaianolides act as antioxidants.
Abstract
Background: Korean mugwort, Artemisia princeps, a plant that has been widely used in diet and traditional medicine throughout East Asia. Methods and Results: Two novel guaian-6,12-olides (1 and 2) and two known compounds (3 and 4) were isolated from Korean mugwort, their chemical structures were established by extensive spec- troscopic analysis and comparison with those of known compounds. The antioxidant capacity of the isolated compounds was evaluated by measuring intracellular reactive oxygen species (ROS) genera- tion in HT-1080 cells and the extent of oxidative damage to genomic DNA purified from HT-1080 cells. Compounds 1 and 3 exhibited outstanding intracellular ROS scavenging activity at a concentra- tion of 10 µM compared to the untreated blank. Particularly, 3 exhibited an exceptional ROS scaveng- ing activity of approximately 67% at a concentration of 1 µM after 2 h. Additionally, all compounds significantly inhibited DNA oxidation induced by hydroxyl radicals at a concentration greater than 1 µM. Among them, 4 showed the most effective protection against DNA damage. Conclusions: Further studies are expected to provide more insight on development of guaian-6,12- olides as potential antioxidants and mechanism by which these types of guaianolides act as antioxidants.
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