결명자 새싹의 재배 방법과 수확 시기에 따른 항산화 활성
Evaluation of Antioxidative Activity of Sicklepod Sprouts Produced by Harvest Times and Cultivation Methods
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Background: To evaluate bioactivities of sicklepod sprout, total polyphenol and flavonoid con- tents, and antioxidative activity based on the amounts of aurantio-obtusin, and obtusifolin were measured during germination and growth. Methods and Results: Sicklepod sprouts were raised in grow boxes. Total polyphenol and flavo- noid contents, and antioxidant activity were determined by established colorimetric methods. Aurantio-obtusin and obtusifolin were quantitatively analyzed by high performance liquid chroma- tography. As the harvest time increased, dry weight increased. The total polyphenol and flavonoid contents were the highest 9 days after sowing (DAS) with respectively valuse of 89.24 ± 3.41 ㎎·gallic acid equivalent/g and 57.31 ± 0.92 ㎎·catechin equivalent/g. At 9 DAS, antioxidant activity as measured using ABTS as the substrate was highest (IC50 164.93 ± 7.52 ㎍/㎖). Aurantio-obtusin and obtusifolin quantities were 1.029 ± 0.045 and 0.146 ± 0.005 ㎎/g, respectively, at 3 DAS. Among growth factors, irrigation interval and sowing density did not lead to significant variations. Considering sprout size and dry weight, a 6 h interval and 2.8 ea/㎠ were chosen. Conclusion: Experimentally determined values were highest at 9 DAS. This finding should serve as a basis for the growth optimization of S. tora sprouts and their potential use as a health func- tional food.
Abstract
Background: To evaluate bioactivities of sicklepod sprout, total polyphenol and flavonoid con- tents, and antioxidative activity based on the amounts of aurantio-obtusin, and obtusifolin were measured during germination and growth. Methods and Results: Sicklepod sprouts were raised in grow boxes. Total polyphenol and flavo- noid contents, and antioxidant activity were determined by established colorimetric methods. Aurantio-obtusin and obtusifolin were quantitatively analyzed by high performance liquid chroma- tography. As the harvest time increased, dry weight increased. The total polyphenol and flavonoid contents were the highest 9 days after sowing (DAS) with respectively valuse of 89.24 ± 3.41 ㎎·gallic acid equivalent/g and 57.31 ± 0.92 ㎎·catechin equivalent/g. At 9 DAS, antioxidant activity as measured using ABTS as the substrate was highest (IC50 164.93 ± 7.52 ㎍/㎖). Aurantio-obtusin and obtusifolin quantities were 1.029 ± 0.045 and 0.146 ± 0.005 ㎎/g, respectively, at 3 DAS. Among growth factors, irrigation interval and sowing density did not lead to significant variations. Considering sprout size and dry weight, a 6 h interval and 2.8 ea/㎠ were chosen. Conclusion: Experimentally determined values were highest at 9 DAS. This finding should serve as a basis for the growth optimization of S. tora sprouts and their potential use as a health func- tional food.
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