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연도별 국내산 품종과 중국산 단삼 품종의 약리활성물질 함량 및 변이

Pharmaceutical Constituents Contents and Deviations in Korean and Chinese Cultivars of Salvia miltiorrhiza Root (Danshen) Followed by Crop Years

김민준|강민지|임정대|이기욱|정진태|한종원|신예림|류병렬
한국약용작물학회|2024.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제32권 제2호|pp. 90-104|DOI 10.7783/KJMCS.2024.32.2.90

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키워드

Color IndexSalvia miltiorrhiza BungeChinese CultivarsCrop YearsKorean CultivarsPharmaceutical ConstituentsSalvianolic acid BTanshinone ⅡA

초록

Background: This study was conducted to determine color parameters, and identify pharmaceutical constituents and phytochemical characteristics of Korean cultivars (Hongdan, Dasan, and Kosan) of Salvia miltiorrhiza root (Danshen) by crop years compared with those of the Chinese cultivar [“Zhongdanyaozhi No. 1 (ZD1)”]. Methods and Results: Redness (a*), yellowness (b*), extract yield, salvianolic acid B (Sal B), and tanshinone ⅡA (TanⅡA) were generally higer in Korean cultivars than in ZD1, implying effective quality control with Korean cultivars in Danshen. Sal B and TanⅡA content were found to be the highest in Korean cultivar ‘Hongdan’ (3-fold higher than ‘ZD1’) in the 2020 crop year. Sal B was chosen as a major markers to assess Korean Danshen quality. However, Sal B detection was apparently incomplete and insufficient for quality control, because Sal B degradation was crop years dependent, Sal B was degraded to some small molecular phenolic acids, such as caffeic acid, protocatechuic aldehyde, lithospermic acid, and danshensu, which were elucidated by liquid chromatography. Analysis of Pearson’s correlation heatmap revealed negative correlation between Sal B and small phenolic acids. The Sal B content of Danshen was correlated to the redness of color parameter, the total terpenoid content, TanⅡA content, suggesting that it would be reasonable to simultaneously detect these characteristics for Danshen quality control. Conclusions: This study provides constructive and meaningful references for the qualitative and quantitative analyses of S. miltiorrhiza root, which can help establish better quality standards.

Abstract

Background: This study was conducted to determine color parameters, and identify pharmaceutical constituents and phytochemical characteristics of Korean cultivars (Hongdan, Dasan, and Kosan) of Salvia miltiorrhiza root (Danshen) by crop years compared with those of the Chinese cultivar [“Zhongdanyaozhi No. 1 (ZD1)”]. Methods and Results: Redness (a*), yellowness (b*), extract yield, salvianolic acid B (Sal B), and tanshinone ⅡA (TanⅡA) were generally higer in Korean cultivars than in ZD1, implying effective quality control with Korean cultivars in Danshen. Sal B and TanⅡA content were found to be the highest in Korean cultivar ‘Hongdan’ (3-fold higher than ‘ZD1’) in the 2020 crop year. Sal B was chosen as a major markers to assess Korean Danshen quality. However, Sal B detection was apparently incomplete and insufficient for quality control, because Sal B degradation was crop years dependent, Sal B was degraded to some small molecular phenolic acids, such as caffeic acid, protocatechuic aldehyde, lithospermic acid, and danshensu, which were elucidated by liquid chromatography. Analysis of Pearson’s correlation heatmap revealed negative correlation between Sal B and small phenolic acids. The Sal B content of Danshen was correlated to the redness of color parameter, the total terpenoid content, TanⅡA content, suggesting that it would be reasonable to simultaneously detect these characteristics for Danshen quality control. Conclusions: This study provides constructive and meaningful references for the qualitative and quantitative analyses of S. miltiorrhiza root, which can help establish better quality standards.

참고문헌 (61)

  1. Biswajit B, "Terpenoids enriched ethanol extracts of aerial roots of Ceriops decandra(Griff.) and Ceriops tagal(Perr.) promote diuresis in mice", Heliyon, 7, pp.e07580~ (2021)
  2. Carregosa D, "Lowmolecular weight metabolites from polyphenols as effectors for attenuating neuroinflammation", Journal of Agricultural and Food Chemistry, 68, pp.1790~1807 (2019)
  3. Chang CL, "Phytochemical characteristics, free radical scavenging activities, and neuroprotection of five medicinal plant extracts", Evidence-Based Complementary and Alternative Medicine, 2012, pp.98429~ (2012)
  4. Chen B, "Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells", Oncology Letters, 20, pp.817~827 (2020)
  5. Chen H, "Qualitative analysis and simultaneous quantification of phenolic compounds in the aerial parts of Salvia miltiorrhiza by HPLCDAD and ESI", MSn, 2011, pp.22, 247~257
  6. Chen X, "Screening of bioactive compounds in Radix Salviae miltiorrhizae with liposomes and cell membranes using HPLC", Journal of Pharmaceutical and Biomedical Analysis, 70, pp.194~201 (2012)
  7. Chen YH, "Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-alpha-treated human aortic endothelial cells", Journal of Cellular Biochemistry, 82, pp.512~521 (2001)
  8. Choi DW, "Statistical difference of production efficiency in medicinal crop farm", Journal of Korean Society for Quality Management, 48, pp.453~462 (2020)
  9. Contreras A, "Proteomic analysis reveals novel insights into tanshinones biosynthesis in Salvia miltiorrhiza hairy roots", Scientific Reports, 9, pp.5768~ (2019)
  10. Di P, "13C Tracer reveals phenolic acids biosynthesis in hairy root cultures of Salvia miltiorrhiza", ACS Chemical Biology, 8, pp.1537~1548 (2013)
  11. Dong Y, "Biosynthesis, total syntheses, and antitumor activity of tanshinones and their analogs as potential therapeutic agents", Natural Product Reports, 28, pp.529~542 (2011)
  12. Guo J, "Cytochrome P450 promiscuity leads to a bifurcating biosynthetic pathway for tanshinones", New Phytologist, 210, pp.525~534 (2016)
  13. Han WS, "Isolation of antimicrobial compounds from Salvia miltiorrhiza Bunge", Korean Journal of Medicinal Crop Science, 12, pp.179~182 (2004)
  14. Ho JHC, "Salvianolic acids : small compounds with multiple mechanisms for cardiovascular protection", Journal of Biomedical Science, 18, pp.30~ (2011)
  15. Hou JJ, "Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard", Journal of Chromatography A, 1218, pp.5618~5627 (2011)
  16. Hu Q, "Sodium tanshinone IIA sulfonate ameliorates ischemia-induced myocardial inflammation and lipid accumulation in Beagle dogs through NLRP3 inflammasome", International Journal of Cardiology, 196, pp.183~192 (2015)
  17. Jenks AA, "Medicinal plant complexes of Salvia subgenus Calosphace : An ethnobotanical study of new world sages", Journal of Ethnopharmacology, 146, pp.214~224 (2013)
  18. Jiang P, "Tanshinone IIA reduces the risk of Alzheimer’s disease by inhibiting iNOS, MMP-2 and NF-κBp65 transcription and translation in the temporal lobes of rat models of Alzheimer’s disease", Molecular Medicine Reports, 10, pp.689~694 (2014)
  19. Joeng JT, "Cultivation and quality characterization of new variety Salvia miltiorrhiza ‘Dasan’", Korean Journal of Medicinal Crop Science, 31, pp.371~376 (2023)
  20. Kan S, "Biotransformation of salvianolic acid B by Fusarium oxysporum f. sp. cucumerinum and its two degradation routes", Natural Product Communications, 7, pp.885~888 (2012)
  21. Research report. Development of quantitative method and analysis of standard compounds from crude drugs(II), "Kang JS.", Korean Food and Drug Administration, 2~ (2004)
  22. Khan RA, "Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper(L. )Hill", Chemistry Central Journal, 6, pp.12~ (2012)
  23. Lee Ay, "Change of quality in Poncirus trifoliata Rafinesque according to storage conditions", Korean Journal of Medicinal Crop Science, 16, pp.188~191 (2008)
  24. Li LN, "Biologically active components from traditional Chinese medicines", Pure and Applied Chemistry, 70, pp.547~554 (1998)
  25. Li MH, "Distribution of tanshinones in the genus Salvia(family Lamiaceae)from China and its systematic significance", Journal of Systematics and Evolution, 48, pp.118~122 (2010)
  26. Li W, "Rapid quantification of phenolic acids in Radix Salvia miltrorrhiza extracts solutions by FT-NIR spectroscopy in transflective mode", Journal of Pharmaceutical and Biomedical Analysis, 52, pp.425~431 (2010)
  27. Li YG, "Advancement in analysis of Salviae miltiorrhizae Radix et Rhizoma(Danshen)", Journal of Chromatography A, 1216, pp.1941~1953 (2009)
  28. Liu H, "Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge", Industrial Crops and Products, 33, pp.84~88 (2011)
  29. Liu XH, "Analysis and identification of Chinese drugs by three-step infrared spectroscopy-A case study of Danshen", Analytical Methods, 4, pp.3344~3350 (2012)
  30. Special crops production statics, "Ministry of Agriculture, Food and Rural Affairs(MAFRA).", Ministry of Agriculture, Food and Rural Affairs, 4~6 (2018)
  31. Special crops production statics, "Ministry of Agriculture, Food and Rural Affairs(MAFRA).", Ministry of Agriculture, Food and Rural Affairs, 4~6 (2021)
  32. Mannelli LDC, "Tanshinones from Salvia miltiorrhiza Bunge revert chemotherapy-induced neuropathic pain and reduce glioblastoma cells malignancy", Biomedicine and Pharmacotherapy, 105, pp.1042~1049 (2018)
  33. Martelanc M, "Separation and identification of some common isomeric plant triterpenoids by thin-layer chromatography and high-performance liquid chromatography", Journal of Chromatography A, 1216, pp.6662~6670 (2009)
  34. Martínez-Cruz O, "Phytochemical profile and nutraceutical potential of chia seeds(Salvia hispanica L. )by ultra high performance liquid chromatography", Journal of Chromatography A, 1346, pp.43~48 (2014)
  35. The Korean pharmacopoeia, "Ministry of Food and Drug Safety(MFDS).", Ministry of Food and Drug Safety, 25~26 (2022)
  36. Origins of herbal medicines and adulterants in Korea and China, "Moon BC", Korea Institute of Oriental Medicine, 35~ (2017)
  37. Nurmi K, "Variation of total phenolic content and individual low-molecular-weight phenolics in foliage of mountain birch trees(Betula pubescens ssp. tortuosa)", Journal of Chemical Ecology, 22, pp.2023~2040 (1996)
  38. Pang H, "Chemical analysis of the herbal medicine Salviae miltiorrhizae Radix et Rhizoma(Danshen)", Molecules, 21, pp.51~ (2016)
  39. Peng L, "Spaceflight environment-induced variation in root yield and active constituents of Salvia miltiorrhiza", Planta Medica, 80, pp.1029~1035 (2014)
  40. Guide of agricultural technology 122. The new resources plants, "Rural Development Administration(RDA).", Rural Development Administration, 193~208 (2018)
  41. Shi D, "Scientific analysis of traditional identification experience of"Redder is better"based on correlation between intrinsic quality and appearance color of Salviae miltiorrhizae Radix et Rhizoma", Chinese Journal of Experimental Traditional Medical Formulae, 24, pp.6~10 (2018)
  42. Shim KM, "Effect of water extract of danshen on bone regeneration of rat calvarial defect model", Korean Journal of Veterinary Research, 50, pp.171~177 (2010)
  43. Song J, "RNAi-mediated suppression of the phenylalanine ammonia-lyase gene in Salvia miltiorrhiza causes abnormal phenotypes and a reduction in rosmarinic acid biosynthesis", Journal of Plant Research, 124, pp.183~192 (2011)
  44. Su Y, "Integrative analysis of metabolome and transcriptome reveals the mechanism of color formation in white root(Salvia miltiorrhiza)", Industrial Crops and Products, 170, pp.113784~ (2021)
  45. Sun A, "Extraction and preparative purification of tanshinones from Salvia miltiorrhiza bunge by high-speed counter-current chromatography", Journal of Chromatography B, 879, pp.1899~1904 (2011)
  46. Wang J, "Study on the electrochemical properties of salvianic acid A sodium and its analytical application", Journal of the Chinese Chemical Society, 59, pp.947~952 (2012)
  47. Wei G, "Correlation analysis between radix Salviae miltiorrhizae colour and contents of active ingredients", Shandong Agricultural Sciences, 2015, pp.59~62 (2015)
  48. Wei Y, "The cardioprotection of dihydrotanshinone I against myocardial ischemia-reperfusion injury via inhibition of arachidonic acid omega-hydroxylase", Canadian Journal of Physiology and Pharmacology, 94, pp.1267~1275 (2016)
  49. Xu M, "Analysis on the stability of total phenolic acids and salvianolic acid B from Salvia miltiorrhiza by HPLC and HPLC-MSn", Natural Product Communications, 3, pp.669~676 (2008)
  50. Yang EJ, "Component analysis and comparison of biological activities of Salvia miltiorrhiza Bunge from different cultivation regions", Journal of the Korean Society of Food Science and Nutrition, 46, pp.929~936 (2017)
  51. Yang F, "Analysis of metabolite accumulation related to pod color variation of Caragana intermedia", Molecules, 24, pp.717~ (2019)
  52. Zhan Z, "Metabolome and transcriptome analyses reveal quality change in the orangerooted Salvia miltiorrhiza(Danshen)from cultivated field", Chinese Medicine, 14, pp.42~ (2019)
  53. Zhang H, "Salvianolic acid A protects RPE cells against oxidative stress through activation of Nrf2", HO-1 signaling, 2014, pp.69, 219~228
  54. Zhao GR, "Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B", Food and Chemical Toxicology, 46, pp.73~81 (2008)
  55. Zhao J, "Advance of CE and CEC in phytochemical analysis(2012–2013)", Electrophoresis, 35, pp.205~224 (2014)
  56. Zhao J, "Diterpenoid tanshinones and phenolic acids from cultured hairy roots of Salvia miltiorrhiza bunge and their antimicrobial activities", Molecules, 16, pp.2259~2267 (2011)
  57. Zhao T, "Specific combination of salvianolic acids as core active ingredients of Danhong injection for treatment of arterial thrombosis and its derived dry gangrene", Frontiers in Pharmacology, 8, pp.361~ (2017)
  58. Zheng X, "Application of multivariate curve resolution method in the quantitative monitoring transformation of salvianolic acid A using online UV spectroscopy and mass spectroscopy", Industrial and Engineering Chemistry Research, 51, pp.3238~3245 (2012)
  59. Zhou GJ, "Post-harvest induced production of salvianolic acids and significant promotion of antioxidant properties in roots of Salvia miltiorrhiza(Danshen)", Molecules, 19, pp.7207~7222 (2014)
  60. Zhou L, "Danshen : An overview of its chemistry, pharmacology, pharmacokinetics, and clinical use", The Journal of Clinical Pharmacology, 45, pp.1345~1359 (2005)
  61. Zhou S, "Protective properties of tanshinone I against oxidative DNA damage and cytotoxicity", Food and Chemical Toxicology, 62, pp.407~412 (2013)

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