Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fem gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely OPG-07496, OPC-151,660 and OPE-181,279. Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from OPG-07496 and OPC-151,660 are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).
참고문헌 (21)
- "A complete full-length non-LTR retrotrans- poson, BMC1, on the W chromosome of the silkworm", 73, pp.353~358 (1998)
- "Identi- fication of novel random amplified polymorphic DNAs (RAPDs) on the W chromosome of the domesticated silk- worm, Bombyx mori, and the wild silkworm, B", 73, pp.243~54 (1998)
- "Construction of RFLP finger- prints in current races of Bombyx mori.", 22, pp.484~486 (2000)
- "The role of the W-chromosome in the sexdetermination of Bombyx mori", pp.245~247 (1933)
- "Con- struction of a linkage map in the silkworm, Bombyx mori, based on SADF and RAPD markers.", 44, pp.476~482 (2001)
- "L1Bm. J. Mol. Evol", 45, pp.253~264 (1997)
- "Genomic sequence of a 320-kb segment of the Z chromosome of Bom- byx mori containing a kettin ortholog", 269, pp.137~149 (2003)
- "Control of the transla-", 30, pp.199~230 (1996)
- "PHAS-I as alink between mitogen-activated protein kinase and transla-tion initiation", pp.653~266 656 (1994)
- "The Dictyostelium develop- mental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res", 5, pp.335~340 (1998)
- "Molecular structure of the copia- like retrotransposable element Yokuzuna on the W chromo- some of the silkworm, Bombyx mori", 73, pp.345~352 (1998)
- "4E-BP3, a new member of the eukaryotic initia- tion factor 4E-binding protein family", 273, pp.14002~14007 (1998)
- "Microsat- ellites in the silkworm, Bombyx mori: abundance, polymor- phism, and strain characterization. Genome", 42, pp.1057~1065 (1999)
- "Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity", 83, pp.681~687 (1999)
- "An amplified fragment length polymorphism map of the silkworm. Genetics", 157, pp.1277~1284 (2001)
- "On the sex discrim-inating method by colouring genes of silkworm eggs induction of translocation between the W and the tenth chro-mosomes", pp.47~50 (1951)
- "Chinese Academy of Agri-cultural Sciences The sericultural science in China" (1991), Scientific and Technical Publishers
- "Regulation of translation during in vitro maturation of bovine oocytes: The Role of MAP kinase, eIF4E (Cap Binding Protein) Phospho- rylation, and eIF4E-BP 1. Biol. Reproduction", 66, pp.1274~1282 (2002)
- "Screening of molecular markers for NPV resistance in Bombyx mori L. (Lepidoptera Bombycidae", 127, pp.134~136 (2003)
- "A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics", 150, pp.1513~1525 (1998)
- "Detachment analysis of the translocated W chromosome shows that the female-specific randomly amplified polymorphic DNA (RAPD) marker, female-218, is derived from the second chromosome fragment region of the translocated W chromosome of the sex-limited p(B) silkworm (Bombyx mori) strain. Hereditas", 138, pp.148~153 (2003)