Molecular Cloning of a Delta-class Glutathione S-transferase Gene from Bombus ignitus
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We describe here the cloning and characterization of a cDNA encoding the glutathione S-transferase (GST) from the bumblebee Bombus ignitus. The Delta-class B. ignitus GST (BiGSTD) gene spans 1668 bp and consists of four introns and five exons that encode 216 amino acid residues with a calculated molecular weight of approximately 24561 Da and a pI of 8.03. The N-terminal domain of BiGSTD has a conserved Ser residue, as well as conserved Lys, Pro, Glu, Ser and Tyr residues that are involved in the GSH-binding site of GST. The BiGSTD showed 60% protein sequence identity to the Bombyx mori GSTT1, 58% to Musca domestica GST, 57% to Drosophila melanogaster GST, and 55% to Anopheles gambiae GST1. BiGSTD was close to the insect-specific Delta class of GSTs in a phylogenetic tree. Northern blot analysis showed that BiGSTD is highly expressed in the fat body and midgut, and less so in the muscles of B. ignitus worker bees.
Abstract
We describe here the cloning and characterization of a cDNA encoding the glutathione S-transferase (GST) from the bumblebee Bombus ignitus. The Delta-class B. ignitus GST (BiGSTD) gene spans 1668 bp and consists of four introns and five exons that encode 216 amino acid residues with a calculated molecular weight of approximately 24561 Da and a pI of 8.03. The N-terminal domain of BiGSTD has a conserved Ser residue, as well as conserved Lys, Pro, Glu, Ser and Tyr residues that are involved in the GSH-binding site of GST. The BiGSTD showed 60% protein sequence identity to the Bombyx mori GSTT1, 58% to Musca domestica GST, 57% to Drosophila melanogaster GST, and 55% to Anopheles gambiae GST1. BiGSTD was close to the insect-specific Delta class of GSTs in a phylogenetic tree. Northern blot analysis showed that BiGSTD is highly expressed in the fat body and midgut, and less so in the muscles of B. ignitus worker bees.
참고문헌 (29)
- Arruda LK., "Induction of IgE antibody responses by glutathione S-transferase from the German cockroach (Blattella germanica)", J Biol Chem., 272, pp.20907~20912 (1997)
- Chelvanayagam G., "Fly fishing for GSTs: a unified nomenclature for mammalian and insect glutathione transferases", Chem Biol Interact, 133, pp.256~260 (2001)
- Choi YS., "Cloning and expression profiling of four antibacterial peptide genes from the bumblebee Bombus ignitus", Comp Biochem Physiol B, 150, pp.141~146 (2008)
- Choi YS., "Bombus ignitus Cu,Zn superoxide dismutase (SOD1): cDNA cloning, gene structure, and up-regulation in response to paraquat, temperature stress, or lipopolysaccharide stimulation", Comp Biochem Physiol B, 144, pp.365~371 (2006)
- Claudianos C., "A deficit of detoxification enzymes: pesticide sensitivity and environmental response in the honeybee", Insect Mol Biol., 15, pp.615~636 (2006)
- Ding Y., "The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression profiles", BMC Genomics, 4, pp.35~ (2003)
- Enayati AA., "Insect glutathione transferases and insecticide resistance", Insect Mol Biol., 14, pp.3~8 (2005)
- Fournier D., "Insect glutathione S-transferases. Biochemical characteristics of the major forms from houseflies susceptible and resistant to insecticides", J Biol Chem., 267, pp.1840~1845 (1992)
- Gui ZZ., "Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution", Eur J Entomol., 105, pp.567~574 (2008)
- Huang HS., "Molecular cloning and heterologous expression of a glutathione S-transferase involved in insecticide resistance from the diamondback moth, Plutella xylostella", Insect Biochem Mol Biol., 28, pp.651~658 (1998)
- Lumjuan N., "Evaluated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti", Insect Biochem Mol Biol., 35, pp.861~871 (2005)
- Mannervik G., "Nomenclature for mammalian soluble glutathione transferases", Methods Enzymol., 401, pp.1~8 (2005)
- Ortelli F., "Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae", Biochem J, 373, pp.957~963 (2003)
- Prapanthadara L., "Partial purification and characterization of glutathione S-transferase involved in DDT resistance from the mosquito Anopheles gambiae", Pest Biochem Physiol, 47, pp.119~133 (1993)
- Ranson H., "Evolution of supergene families associated with insecticide resistance", Science, 298, pp.179~181 (2002)
- Ranson H., "Cloning and characterization of two glutathione S-transferases from a DDT-resistant strain of Anopheles gambiae", Biochem J., 324, pp.97~102 (1997)
- Ranson H., "Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae", Biochem J., 359, pp.295~304 (2001)
- Rossjohn J., "Human theta class glutathione transferase: the crystal structure reveals a sulfate-binding pocket within a buried active site", Structure, 6, pp.309~322 (1998)
- Sheehan D., "Structure, function and evolution of glutathione transferases: implications for classfication of non-mammalian members of an ancient enzyme superfamily", Biochem J., 360, pp.1~16 (2001)
- Snyder MK., "Glutathione Stransferases from larval Manduca sexta midgut: sequence of two cDNAs and enzyme induction", Insect Biochem Mol Biol., 25, pp.455~465 (1995)
- Swofford DL., "PAUP: phylogenetic analysis using parsimony, ver 4.0", Sinauer, ~ (2000)
- Valles SM., "Purification, biochemical characterization, and cDNA cloning of a glutathione S-transferase from the red imported fire ant, Solenopsis invicta", Insect Biochem Mol Biol., 33, pp.981~988 (2003)
- Vontas JG., "Glutathione Stransferases as antioxidant defense against confer pyrethroid resistance in Nilaparvata lugens", Biochem J., 357, pp.65~72 (2001)
- Wang WS., "Molecular cloning of a glutathione S-transferase overproduced in an insecticide- resistant strain of the housefly (Musca domestica)", Mol Gen Genet, 227, pp.260~266 (1991)
- Yamamoto K., "Expression and characterization of a sigma-class glutathione S-transferase of the fall webworm, Hyphantria cunea", Biosci Biotechnol Biochem, 71, pp.553~560 (2007)
- Yamamoto K., "Identification of a sigma-class glutathione-S-transferase from the silkworm, Bombyx mori", J Appl Entomol, 130, pp.515~522 (2006)
- Yamamoto K., "Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori", Comp Biochem Physiol B, 141, pp.340~346 (2005)
- 윤형주, "Colony Developmental Characteristics of the Bumblebee Queen, Bombus ignitus by the First Oviposition Day", International Journal of Industrial Entomology, 8(2), pp.139~143 (2004), 한국잠사학회
- Yoon HJ., "Temperature and humidity favorable for colony development of the indoor-reared bumblebee, Bombus ignitus", Appl Entomol Zool, 37, pp.419~423 (2002)