Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, Paecilomyces tenuipes, Cordyceps militaris and Cordyceps pruinosa
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain),P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288nucleotides encoding 713 amino acid residues,whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.
Abstract
Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain),P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288nucleotides encoding 713 amino acid residues,whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.
참고문헌 (41)
- Arrigo AP, "Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells", Dev Biol, 78, pp.86~103 (1980)
- Bork P, "An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin and Hsp70 heat shock proteins", Proc Natl Acad Sci USA, 89, pp.7290~7294 (1992)
- Buchberger A, "The chaperone function of DNA K requires the coupling of ATPase activity with substrate binding through residue E171", EMBO J, 13, pp.1687~1695 (1994)
- Davis TWU, "Heat", stress responses, 2004, pp.2, 343~347
- Flaherty KM, "Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein", Proc. Natl. Acad. Sci USA, 88, pp.5041~5045 (1991)
- Fujita T, "Fungal metabolite a potent immuno suppressive activity found in Isariasinclaini metabolite", J Antibiot, 47, pp.208~215 (1994)
- Furuya T, "N6-(2-HYDROXYETHYL)adenosine, a biologically active compound from cultured mycelia of Codyceps and Isaria species", Phytochemistry, 22, pp.2509~2512 (1983)
- Holmgren R, "Studies of cloned sequences from 4 Drosophila heat shock loci", Cell, 18, pp.1359~1370 (1979)
- Hsu CH, "Effects of the immune modulatory agent Cordyceps militaris on airway inflammation in a mouse asthma model", Pediat Neonatol, 49, pp.171~178 (2008)
- Im DS, "Linking chinese medicine and G-protein-coupled receptors", Trends Pharmacol Sci, 24, pp.2~4 (2003)
- Kapoor M, "The HSP70 gene family of Saccharomyces cerevisiae of Neurospora crassa: cloning, sequence analysis, expression, and genetic mapping of the major stress-inducible member", J Bacteriol, 177, pp.212~221 (1995)
- Khan MA, "Cordyceps mushroom: a potent anticancer nutraceutical", Open Nutra J, 3, pp.179~183 (2010)
- Kim HG, "Cordyceps pruinosa extracts induce apoptosis of HeLa cells by a caspase dependent pathway", J Ethnopharmacol, 128, pp.342~351 (2010)
- Kneifel H, "Ophiocordin, an antifungal antibiotic of cordyceps ophioglossoides", Arch Microbiol, 113, pp.121~130 (1977)
- Kumar S, "MEGA: Molecular evolutionary genetics analysis software for microcomputers", Comput Appl Biosci, 10, pp.189~191 (1994)
- Kuo MC, "Immunomodulatory effect of exo-polysaccharides from submerged cultured Cordyceps sinensis: enhancement of cytokine synthesis, CD11b expression, and phagocytosis", Appl Microbiol Biotechnol, 75, pp.769~775 (2007)
- Eunju Park, "Effects of Paecilomyces tenuipes Cultivated in Egg Yolk on Lipid Metabolism in Rats on a High Fat-Cholesterol Diet", Journal of Medicinal Food, 9(2), pp.214~222 (2006), 한국식품영양과학회
- Lemaux P, "Transient rates of synthesis of individual polypeptides in E.coli following temperature shifts", Cell, 13, pp.427~434 (1978)
- Lindquist S, "The heat shock response", Annu Rev Biochem, 55, pp.1151~1191 (1986)
- Ya-qi Liu, "Molecular cloning, expression and characterization of heat shock protein gene from Paecilomyces tenuipes Jocheon-1", 석사, pp.2011
- Montefiori DC, "Phosphorothioate and cordycepin analogues of 2,5-oligo adenylate: Inhibition of human immunodeficiency type 1 reverse transcriptase and infection in vitro", Proc Natl Acad Sci USA, 86, pp.7191~7194 (1989)
- Nakamura K, "Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis on lewis lung carcinoma and B16 melanoma cells in syngeneic mice", Jpn J Parmacol, 79, pp.335~341 (1999)
- Ohta Y, "In vivo anti-influenza virus activity of an immunomodulatory acidic polysaccharide isolated from Cordyceps militaris grown on germinated soybeans", Agric Food Chem, 55, pp.10194~10199 (2007)
- 김익수, "Molecular cloning, expression, and characterization of the Cu,Zn superoxide dismutase (SOD1) gene from the entomopathogenic fungus Cordyceps militaris", MYCOLOGIA, 97(1), pp.130~138, NEW YORK BOTANICAL GARDEN
- Sang Eun Park, "Antitumor Activity of Water Extracts From Cordyceps Militaris in NCI-H460 Cell Xenografted Nude Mice", Journal of Acupuncture & Meridian Studies, 2(4), pp.294~300 (2009), 세계약침학회
- Pelham H, "DNA sequences required for transcriptional regulation of the Drosophila heat-shock gene in monkey cells and Xenopus oocytes. In Heat Shock from Bacteria to Man" (1982), Cold Spring Harbor Press
- Peterson NS, "Heat shock proteins in comprehensive insect", Physiol Biochem Pharmacol, 10, pp.347~365 (1985)
- Plesofsky-Vig N, "Characterization of an 88-kDa heat shock protein of Neurosporacrassathat interacts with Hsp30", J Biol Chem, 273, pp.11335~11341 (1998)
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Samson RA, "Atlas of entomopathogenic fungi" (1988), Springer-Verlag
- Shimizu D, "Illustrated vegetable wasps and plant worms in colour", IE-NO-HIKARI Associantion. Japan, ~ (1997)
- Snutch TP, "The Caenorhabditis elegans Hsp70 gene family: a molecular characterization", Gene, 64, pp.241~255 (1988)
- Stefani RMP, "An unique intron-containing intron-containing Hsp70 gene induced by heat shock and during sporulation in the aquatic fungus Blastocladiella emersonii", Gene, 152, pp.19~26 (1995)
- Sung JM, "Distribution and taxonomy of entomopathogenic fungal species from Korea", KJM, 25, pp.239~252 (1997)
- Thompson JD, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res, 22, pp.4673~4680 (1994)
- Tissieres A, "Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs", J Mol Biol, 84, pp.389~398 (1974)
- Xu RH, "Effects of Cordyceps sinensis on natural killer activity and colony formation of B16 melanoma", Chin Med J, 105, pp.97~101 (1992)
- Yin YY, "Bioactive compounds from Paecilomyces tenuipes regulating the function of the hypothalamo-hypophyseal system axis in chronic unpredictable stress rats", Chin Med J, 120, pp.1088~1092 (2007)
- Yoshida J, "Antitumor activity of an extract of Cordyceps sinensis(Berk.) Sac. against murine tumor cell lines", J Exp Med, 59, pp.157~161 (1989)
- Zhang AI, "Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps militaris", Chem Res Chinese Univ, 26, pp.798~802 (2010)
- Zhu, JS, "The scientific rediscovery of a precious ancient Chinese herbal regimen: Cordyceps sinensis: part1", J Altern Complement Med, 4, pp.289~303 (1998)