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Isoelectric Focusing of the Hemolymph Proteins of the Silkworm, Bombyx mori

Teodora Staykova|Petar Popov|Dimitar Grekov|Petia Terzieva
한국잠사학회 (Korean Society Of Sericultural Science)|2004.03|International Journal of Industrial Entomology and Biomaterials|제8권 제1호|pp. 117-121

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Abstract

Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and interraces hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5 ~ 6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 ~ 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and interrace polymorphism in silkworm.

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관련 논문AI추천

Electrophoretic Analysis of Haemolymph Proteins during Silkworm (Bombyx mori L.) OntogenesisTeodora Staykova한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2007 · 제14권 제1호Electrophoretic Analysis of Nonspecific Esterases in Silkworm (Bombyx mori L.) Female Genital Organs and EggsDimitar Grekov, Mihail Panayotov, Teodora Staykova한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제9권 제1호Molecular Cloning of a cDNA Encoding Putative ApolipophorinⅢ from the Silkworm, Bombyx mori강석우, 구태원, 권오유, 김성완, 윤은영, 최광호, 황재삼한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2003 · 제7권 제2호Analysis of Diapause-Associated Changes in Silkworm Egg Proteins강석우, 고현정, 김영태, 김현수, 성수일, 이봉희, 이상몽, 장종수, 황재삼한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제9권 제1호Shifts in Protein Metabolism in Hemolymph and Fat Body of the Silkworm, Bombyx mori L. in Response to Fluoride ToxicityJayaprakash, S. Ramakrishna한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2007 · 제15권 제1호Adaptive Significance of Changes in the Biochemical Constituents of Haemolymph to Imposed Thermal Stress on Seleted Races of the Silkworm, Bombyx mori LFirdose Ahmad Malik, Y. Srinivasa Reddy한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2006 · 제12권 제2호

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