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Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Mulberry Genome Analysis

Kunjupillai Vijayan
한국잠사학회 (Korean Society Of Sericultural Science)|2005.06|International Journal of Industrial Entomology and Biomaterials|제10권 제2호|pp. 79-86

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Abstract

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification,genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry,for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

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관련 논문AI추천

Evaluation of ISSR and RAPD Markers for the Detection of Genetic Diversity in Mulberry (Morus spp.)B. Saratchandra, B. Surendra Nath, M. Venkateswarlu, S. Raje Urs한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제9권 제2호Molecular Markers and Their Application in Mulberry BreedingKunjupillai Vijayan한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2007 · 제15권 제2호Analysis of the Genetic Relationship among Mulberry (Morus spp.) Cultivars Using Inter-Simple Sequence Repeat (ISSR) Markers노시갑, Eun-Ju Park, Gyoo-Byung Sung, Min-Uk Kang, Myoung-Seob Choi한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2020 · 제41권 제2호Cryopreservation of Mulberry Germplasm Core Collection and Assessment of Genetic Stability through ISSR MarkersA. Ananda Rao, C. K. Kamble, D. Velu, Rekha Chaudhury, R. P. Saraswat, Suseel Kumar한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2007 · 제15권 제1호Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)Cheng Lu, Minghui Li, Muwang Li, Xuexia Miao, Yongping Huang한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2005 · 제11권 제1호Identification of Genetic Relationships Among Morus alba Genotypes Based on RAPD and ISSR Fingerprinting이양수, 차효정, 최태기, Duraisamy Kalpana한국자원식물학회 · 한국자원식물학회지2011 · 제24권 제6호

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