High-level Expression, Polyclonal Antibody Preparation and Bioinformatics Analysis of Bombyx mori Nucleopolyhedrovirus orf47 Encodes Protein
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.
Abstract
Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.
참고문헌 (18)
- Acharya, A., "“Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus.”", Virology, 299(2), pp.213~224 (2002)
- Ayres, M. D., "“The complete DNA sequence of Autographa californica nuclear polyhedrosis virus.”", Virology, 202(2), pp.586~605 (1994)
- Baluchamy, S., "“Characterization of a cyclin homolog from Bombyx mori nucleopolyhedrovirus.”", Virus Res, 108(1-2), pp.69~81 (2005)
- Chen, H. Q., "“Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus.”", FEBS Lett, 581(30), pp.5836~5842 (2007)
- Daimon, T., "“Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus.”", Virus Res, 124(1-2), pp.168~175 (2007)
- Du, M. F., "“Characterization of a late gene, ORF60 from Bombyx mori nucleopolyhedrovirus.”", J Biochem Mol Biol, 39(6), pp.737~742 (2006)
- Gomi, S., "“Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus.”", J Gen Virol, 80(Pt 5), pp.1323~1337 (1999)
- Herniou, E. A., "“Use of whole genome sequence data to infer baculovirus phylogeny.”", J Virol, 75(17), pp.8117~8126 (2001)
- Hu, Y. X., "“Get effective polyclonal antisera in one month.”", Cell Res, 12(2), pp.157~160 (2002)
- Imai, N., "“Bombyx mori nucleopolyhedrovirus orf8 encodes a nucleic acid binding protein that colocalizes with IE1 during infection.”", Arch Virol, 149(8), pp.1581~1594 (2004)
- Jehle, J. A., "“On the classification and nomenclature of baculoviruses: a proposal for revision.”", Arch Virol, 151(7), pp.1257~1266 (2006)
- J. Sambrook, "Molecular Cloning: A Laboratory Manual" (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
- Katsuma, S., "“In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf.”", Virology, 355(1), pp.62~70 (2006)
- Lu, M., "“The p95 gene of Bombyx mori nuclear polyhedrosis virus: temporal expression and functional properties.”", J Virol, 72(6), pp.4789~4797 (1998)
- Park, K. J., "“Prediction of protein subcellular locations by support vector machines using compositions of amino acids and amino acid pairs.”", Bioinformatics, 19(13), pp.1656~1663 (2003)
- Shijuku, T., "“Expression of chaA, a sodium ion extrusion system of Escherichia coli, is regulated by osmolarity and pH.”", Biochim Biophys Acta, 1556(2-3), pp.142~148 (2002)
- Wang, F., "“The translational and transcriptional initiation sites of BmNPV lef-7 gene.”", Virus Genes, 33(3), pp.351~357 (2006)
- Xu, H. J., "“Bombyx mori nucleopolyhedrovirus ORF79 encodes a 28-kDa structural protein of the ODV envelope.”", Arch Virol, 151(4), pp.681~695 (2006)