The Influence of Challenge on Cathepsin B and D Expression Patterns in the Silkworm Bombyx mori L.
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that Bombyx mori cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RTPCR was performed to detect cathepsin gene expression at the transcription level when challenged by B. mori nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the 5th instar,and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the 5^(th) instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.
Abstract
Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that Bombyx mori cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RTPCR was performed to detect cathepsin gene expression at the transcription level when challenged by B. mori nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the 5th instar,and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the 5^(th) instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.
참고문헌 (36)
- Ahn JE, "CmCatD, a cathepsin D-like protease has a potential role in insect defens against a phytocystatin", J Insect Physiol, 55, pp.678~685 (2009)
- Attardo GM, "Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans", Insect Mol Biol, 15, pp.411~424 (2006)
- 후지사키코조, "Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 36(1), pp.25~36, PERGAMON-ELSEVIER SCIENCE LTD
- Brunelle F, "Protein hydrolysis by Colorado potato beetle, Leptinotarsa decemlineata, digestive proteases: the catalytic role of cathepsin D", Arch Insect Biochem Physiol, 42, pp.88~98 (1999)
- Carnevali O, "Changes of lysosomal enzyme activities in sea bass (Dicentrarchus labrax) eggs and developing embryos", Aquaculture, 202, pp.249~256 (2001)
- Cho WL, "Cloning of cDNA for mosquito lysosomal aspartic protease-sequence-analysis of an insect lysosomal-enzyme similar to cathepsin D and E", J Biol Chem, 267, pp.21823~21829 (1992)
- Cho WL, "Mosquito cathepsin Blike protease involved in embryonic degradation of vitellin is produced as a latent extraovarian precursor", J Biol Chem, 274, pp.13311~13321 (1999)
- De Gregorio E, "Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays", Proc Nat Acad Sci USA, 98, pp.12590~12595 (2001)
- Duve C, "Lysosomes revisited", Eur J Biochem, 137, pp.391~397 (1983)
- Fehrenbacher N, "Lysosomes as targets for cancer therapy", Cancer Res, 65, pp.2993~2995 (2005)
- Gui ZZ, "Functional role of aspartic proteinase cathepsin D in insect metamorphosis", BMC DEVELOPMENTAL BIOLOGY, 6(1), pp.49~49 (2006), BIOMED CENTRAL LTD
- Gui ZZ, "Effects of insect viruses and pesticides on glutathione Stransferase activity and gene expression in Bombyx mori L", J Econ Entomol, 102, pp.1591~1598 (2009)
- Heidtmann HH, "Secretion of a latent, acid activatable cathepsin L precursor by human non-small cell lung cancer cell lines", Oncol Res, 5, pp.441~451 (1993)
- Kurata S, "The 29-kDa hemocyte proteinase dissociates fat body at metamorphosis of Sarcophaga", Dev Biol, 153, pp.115~121 (1992)
- Lee KS, "Expression prole of cathepsin B in the fat body of Bombyx mori during metamorphosis", Comp Biochem Physiol B, 154, pp.188~194 (2009)
- Metcalf P, "crystal structures for cathepsin D: the lysosomal targeting signal and active site", EMBO J, 12, pp.1293~1302 (1993)
- Nakagawa T, "Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus", Science, 280, pp.450~453 (1998)
- Nakanishi H, "Involvement of nitric oxide released from microglia-macrophages in pathological changes of cathepsin D-decient mice", J Neurosci, 21, pp.7526~7533 (2001)
- Rabossi A, "Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis", Arch Insect Biochem Physiol, 57, pp.51~67 (2004)
- Saftig P, "Mice decient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells", EMBO J, 14, pp.3599~3608 (1995)
- Schefe JH, "Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula", J Mol Med, 84, pp.901~910 (2006)
- Serbielle CI, "Identification of parasite-responsive cysteine pro teases in Manduca sexta", Biol Chem, 390, pp.493~502 (2009)
- Shiba H, "Involvement of cathepsin B- and L-Like proteinases in silk gland histolysis during metamorphosis of Bombyx mori", Arch Insect Biochem Physiol, 390, pp.28~34 (2001)
- Shompole S, "Cathepsin B-like cysteine proteases confer intestinal cysteine protease activity in Haemonchus contortus", J Biol Chem, 276, pp.2928~2934 (2001)
- Silva CP, "Comparison between the levels of aspartic and cysteine proteinases of the larval midguts of Callosobruchus maculatus (F) and Zabrotes subfasciatus (Boh) (Coleoptera, Bruchidae)", Comp Biochem Physiol B, 99, pp.529~533 (1991)
- Sloane BF, "Membrane association of cathepsin B can be induced by transfection of human breast epithelial cells with c-Harasoncogene", J Cell Sci, 107, pp.373~384 (1994)
- Snigirevskaya ES, "Secretory and internalization pathways of mosquito yolk protein precursors", Cell Tissue Res, 290, pp.129~142 (1997)
- Takahashi N, "Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)", FEBS Lett, 334, pp.153~157 (1993)
- Tsuji H, "Identification and characterization of lysosomal enzymes involved in the proteolysis of phenobarbital- inducible cytochrome P450", Biol Pharm Bull, 17, pp.568~571 (1994)
- Turk B, "Lysosomal cysteine proteases: more than scavengers", Biochim Biophys Acta, 1477, pp.98~111 (2000)
- Urbich C, "Cathepsin L is required for endothelial progenitor cell-induced neovascularization", Nat Med, 11, pp.206~213 (2005)
- Xu YS, "Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori", Comp Biochem Physiol B, 130, pp.393~399 (2001)
- Yang XM, "Cathepsin B-like proteinase is involved in the decomposition of the adult fat body of Helicoverpa armigera", Arch Insect Biochem Physiol, 62, pp.1~10 (2006)
- Yang XM, "Expression and function of cathepsin B-like proteinase in larval hemocytes of Helicoverpa armigera during metamorphosis", Arch Insect Biochem Physiol, 64, pp.164~174 (2007)
- Zhong D, "Expression of cathepsins B, D and L in mouse corneas infected with Pseudomonas aeruginosa", Eur J Biochem, 268, pp.6408~6416 (2001)
- Zou FM, "Expression profiles of glutatione S-transferase (GST) genes in larval midgut of Bombyx mori exposed to insect hormones", Mol Biol Rep, 38, pp.639~647 (2011)
