Light and Electron Microscopic Study on the Development of Nosema Bombyics Naegeli in the Midgut of Silkworm Bombyx Mori L.
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Infection effect of Nosema bombycis on the midgut of silkworm Bombyx mori and subsequent appearance of spores and the performance of larvae was studied. Autopsy of larvae showed white pustules on the surface of midgut at 5 days of post infection (pi). At later stage, important organs like midgut, silk gland and gonads reduced in size and all these organs showed white pustules. Light microscope observation of pustules revealed enormous spores. Spore multiplication was at a faster rate in young larvae. Infection of the adult larvae resulted in pebrinized pupa and moths. Larval weight, cocoon weight and cocoon shell ratio reduced as the post infection period increased. Transverse sections of midgut showed N. bombycis infection limited to a few columnar cells at 3-5 days of pi. At 7days pi, cell volume increased, cells were swollen and elongated. Heavily infected cells looked like sacks filled with parasite and the apical region of certain cells were bulging into the gut lumen. Later at 8-9 days of pi, spores or its developing stages leaked into the lumen either freely or enclosed within the globules of host cytoplasm. Besides columnar cells, development of N. bombycis was observed in the regenerative cells and rarely in goblet cells. Development of N. bombycis was also observed in both longitudinal and circular muscles at the late pi period. The histopathological changes, deformities and spore production time in the host were all influenced by the spore dosage and age of the host.
Abstract
Infection effect of Nosema bombycis on the midgut of silkworm Bombyx mori and subsequent appearance of spores and the performance of larvae was studied. Autopsy of larvae showed white pustules on the surface of midgut at 5 days of post infection (pi). At later stage, important organs like midgut, silk gland and gonads reduced in size and all these organs showed white pustules. Light microscope observation of pustules revealed enormous spores. Spore multiplication was at a faster rate in young larvae. Infection of the adult larvae resulted in pebrinized pupa and moths. Larval weight, cocoon weight and cocoon shell ratio reduced as the post infection period increased. Transverse sections of midgut showed N. bombycis infection limited to a few columnar cells at 3-5 days of pi. At 7days pi, cell volume increased, cells were swollen and elongated. Heavily infected cells looked like sacks filled with parasite and the apical region of certain cells were bulging into the gut lumen. Later at 8-9 days of pi, spores or its developing stages leaked into the lumen either freely or enclosed within the globules of host cytoplasm. Besides columnar cells, development of N. bombycis was observed in the regenerative cells and rarely in goblet cells. Development of N. bombycis was also observed in both longitudinal and circular muscles at the late pi period. The histopathological changes, deformities and spore production time in the host were all influenced by the spore dosage and age of the host.
참고문헌 (17)
- Akai H, "An electron microscopic study of the alimentary canal of the silkworm, Bombyx mori L. I – The ultra structure of midgut epithelium", Bull Seric Exp Sta, 24, pp.303~344 (1970)
- Canning, Hulls, "A microsporidian infection of Anopheles gambiaeI Giles from Tanzania, interpretation of its mode of transmission and notes on Nosema infection in mosquitoes", J Invertebr Pathol, 17, pp.531~539 (1970)
- Cantwell GE, "Honey bee diseases, parasite and pests, In Insect diseases Vol II", pp.501~547 (1974), Marcel Dekker
- Cole RJ, "the application of the triangulation method to the purification of Nosema spores from insect tissues", J Invertebr Pathol, 15, pp.193~195 (1970)
- Fukuda T, "Correlation between the mulberry leaves taken by the silkworm and the silk preotein in the silkgland and silk filament", Bull Seric Exp Stn, 15, pp.605~610 (1960)
- Krishnaswami S, "New method of Silkworm Rearing" (1990), Central Silk Board Publ
- Karnovsky MH, "formaldehyde–glutaraldehyde fixative of high osmolarity for use in electron microscopy", J Cell Biol, 27, pp.137~138 (1965)
- Mathavan S, "Histochemical and histopathological studies on the midgut epithelium of Bombyx mori larvae affected by Bacillus sphaericus", Sericologia, 31, pp.403~411 (1989)
- Noamani KR, "Assessment of different cocoon characters of the multivoltine silkworm (Bombyx mori) as influenced by pebrine disease", Indian J Seric, 10, pp.96~100 (1971)
- Patil CS, "Faecal pellet examination of silkworm Bombyx mori as a diagnostic method to detect pebrine disease", J Expt Zool, 4, pp.311~315 (2001)
- Reynold ES, "The use of lead citrate at high pH as an electron stain in electron microscopy", J Cell Biol, 17, pp.208~212 (1963)
- Sacchi VF, "Amino acid absorption, In Biology of Insect Midgut Lehane MJ", pp.265~292 (1996), Chapman and Hall
- Shabanov NM, "Studies on the nosematosis of bees in Hungary", Biological Aspects of Nosema Disease- Symposium of bee biology and pathology, pp.88~97 (1976)
- Singh GP, "study on the larval silk gland and shell weight in the silkworm Bombyx mori L", Entomon, 21, pp.277~279 (1996)
- Sinha USP, "Changes in protein content in the haemolymph of healthy and pebrine infected larvae and pupae of Antheraea mylitta D", Indian J Seric, 30, pp.157~158 (1991)
- Thomson HM, "A list and brief description of the microsporidia infecting insects", J Invertebr Pathol, 2, pp.346~385 (1960)
- Wafa AK, "Histopathological studies of Nosema bombycis Naegeli infecting the silkworm, Bombyx mori", Bull Soc Entomol Egypte, 55, pp.259~274 (1971)