우유단백 항원의 열처리 및 가수분해에 따른 단백의 분포와 항원성 변화
The effect of heat treatment or hydrolysis on cow’s milk protein distributions and antigenicities
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Purpose: Cow’s milk protein is one of the most common and strongest food allergen. We investigated the effects of heat treatment on the distribution and antigenicities of major allergens from cow’s milk. We also compared the protein distribution and antigenicities among cow’s milk formula and its substitutes. Methods: We heated α-casen, β-lactoglobulin (BLG), α-lactalbumin (ALA), and crude extract of cow’s milk in 100°C boiling water for 1 hour. We prepared crude extracts from cow’s milk formula, partially hydrolyzed milk formula (pHF) and extensively hydrolyzed milk formula (eHF). The protein compositions of all the samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenicities were determined by IgE immunoblotting with pooled serum collected from 11 patients with milk allergy. Results: After heating, no significant alteration was found in casein, and the aggregates of ALA and BLG were detected with molecular weights of about 30 and 45 kDa, respectively. The antigenicities of newly detected aggregates were increased. The new aggregates of BLG with increased antigenicities were also found in heated milk total protein. Major milk allergens were not found in pHF, and residual components with a molecular weight below 10 KDa did not show IgE-binding activity. We failed to observe the residual components and antigenicities of eHF. Conclusion: Changes in protein distribution and antigenicity of milk total protein induced by heat treatment may not be significantly different from those of each major allergen. The residual components of pHF could have little IgE-binding capacity, and there may be few or no antigenic components in eHF.
Abstract
Purpose: Cow’s milk protein is one of the most common and strongest food allergen. We investigated the effects of heat treatment on the distribution and antigenicities of major allergens from cow’s milk. We also compared the protein distribution and antigenicities among cow’s milk formula and its substitutes. Methods: We heated α-casen, β-lactoglobulin (BLG), α-lactalbumin (ALA), and crude extract of cow’s milk in 100°C boiling water for 1 hour. We prepared crude extracts from cow’s milk formula, partially hydrolyzed milk formula (pHF) and extensively hydrolyzed milk formula (eHF). The protein compositions of all the samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenicities were determined by IgE immunoblotting with pooled serum collected from 11 patients with milk allergy. Results: After heating, no significant alteration was found in casein, and the aggregates of ALA and BLG were detected with molecular weights of about 30 and 45 kDa, respectively. The antigenicities of newly detected aggregates were increased. The new aggregates of BLG with increased antigenicities were also found in heated milk total protein. Major milk allergens were not found in pHF, and residual components with a molecular weight below 10 KDa did not show IgE-binding activity. We failed to observe the residual components and antigenicities of eHF. Conclusion: Changes in protein distribution and antigenicity of milk total protein induced by heat treatment may not be significantly different from those of each major allergen. The residual components of pHF could have little IgE-binding capacity, and there may be few or no antigenic components in eHF.
참고문헌 (37)
- Savilahti E, "Allergenicity of cow milk proteins", J Pediatr, 121(5 Pt 2), pp.S12~S20 (1992)
- Høst A, "Clinical course of cow's milk protein allergy", intolerance and atopic diseases in childhood, 2002, pp.13(5), 23~28
- Venter C, "Incidence of parentally reported and clinically diagnosed food hypersensitivity in the first year of life", J Allergy Clin Immunol, 117, pp.1118~1124 (2006)
- Hill DJ, "Natural history of cows' milk allergy in children: immunological outcome over 2 years", Clin Exp Allergy, 23, pp.124~131 (1993)
- Skripak JM, "The natural history of IgE-mediated cow's milk allergy", J Allergy Clin Immunol, 120, pp.1172~1177 (2007)
- Saarinen KM, "Clinical course and prognosis of cow's milk allergy are dependent on milk-specific IgE status", J Allergy Clin Immunol, 116, pp.869~875 (2005)
- Wood RA, "The natural history of milk allergy in an observational cohort", J Allergy Clin Immunol, 131, pp.805~812 (2013)
- Sackesen C, "Cow's milk allergy as a global challenge", Curr Opin Allergy Clin Immunol, 11, pp.243~248 (2011)
- Gjesing B, "Allergen-specific IgE antibodies against antigenic components in cow milk and milk substitutes", Allergy, 41, pp.51~56 (1986)
- Sicherer SH, "Cow's milk protein-specific IgE concentrations in two age groups of milk-allergic children and in children achieving clinical tolerance", Clin Exp Allergy, 29, pp.507~512 (1999)
- Bu G, "Milk processing as a tool to reduce cow's milk allergenicity: a mini-review", Dairy Sci Technol, 93, pp.211~223 (2013)
- Kilshaw PJ, "Effects of heat treatment of cow's milk and whey on the nutritional quality and antigenic properties", Arch Dis Child, 57, pp.842~847 (1982)
- Wal JM, "Bovine milk allergenicity", Ann Allergy Asthma Immunol, 93(5 Suppl 3), pp.S2~S11 (2004)
- Davis PJ, "How can thermal processing modify the antigenicity of proteins?", Allergy, 56(67), pp.56~60 (2001)
- Singh B, "Minimum peptide sequences necessary for priming and triggering of humoral and cell-mediated immune responses in mice: use of synthetic peptide antigens of defined structure", J Immunol, 124, pp.1336~1343 (1980)
- Chung CS, "FDA's health claim review: whey-protein partially hydrolyzed infant formula and atopic dermatitis", Pediatrics, 130, pp.e408~e414 (2012)
- Isolauri E, "Efficacy and safety of hydrolyzed cow milk and amino acid-derived formulas in infants with cow milk allergy", J Pediatr, 127, pp.550~557 (1995)
- de Boissieu D, "Allergy to extensively hydrolyzed cow milk proteins in infants: identification and treatment with an amino acid-based formula", J Pediatr, 131, pp.744~747 (1997)
- de Boissieu D, "Allergy to extensively hydrolyzed cow's milk proteins in infants: safety and duration of amino acid-based formula", J Pediatr, 141, pp.271~273 (2002)
- Goldman AS, "Milk allergy. I: oral challenge with milk and isolated milk proteins in allergic children", Pediatrics, 32, pp.425~443 (1963)
- Huang F, "Extensively heated milk and egg as oral immunotherapy", Curr Opin Allergy Clin Immunol, 12, pp.283~292 (2012)
- Maleki SJ, "Structural and functional alterations in major peanut allergens caused by thermal processing", J AOAC Int, 87, pp.1475~1479 (2004)
- Gruber P, "Influence of the maillard reaction on the allergenicity of rAra h 2, a recombinant major allergen from peanut (Arachis hypogaea), its major epitopes, and peanut agglutinin", J Agric Food Chem, 53, pp.2289~2296 (2005)
- 김태형, "이유기에 섭취한 미음에 의해 발견된 쌀 알레르기 1례", Allergy Asthma & Respiratory Diseases, 17(2), pp.149~154 (2007), 대한 소아알레르기 호흡기학회
- Docena GH, "Identification of casein as the major allergenic and antigenic protein of cow's milk", Allergy, 51, pp.412~416 (1996)
- Caubet JC, "Tility of casein-specific IgE levels in predicting reactivity to baked milk", J Allergy Clin Immunol, 131, pp.222.e1-4~224.e1-4 (2013)
- Nowak-Wegrzyn A, "Tolerance to extensively heated milk in children with cow's milk allergy", J Allergy Clin Immunol, 122, pp.342.e1-2~347.e1-2 (2008)
- Baldo B, "Milk allergies", Aust J Dairy Technol, 39, pp.120~128 (1984)
- Ehn BM, "Modification of IgE binding during heat processing of the cow's milk allergen beta-lactoglobulin", J Agric Food Chem, 52, pp.1398~1403 (2004)
- Kleber N, "Antigenic response of β-lactoglobulin in thermally treated bovine skim milk and sweet whey", Milchwissenschaft, 62, pp.121~124 (2007)
- Bu G, "Effect of heat treatment on the antigenicity of bovine α-lactalbumin and β-lactoglobulin in whey protein isolate", Food Agric Immunol, 20, pp.195~206 (2009)
- Anema SG, "Association of denatured whey proteins with casein micelles in heated reconstituted skim milk and its effect on casein micelle size", J Dairy Res, 70, pp.73~83 (2003)
- Corredig M, "The mechanisms of the heat-induced interaction of whey proteins with casein micelles in milk", Int Dairy J, 9, pp.233~236 (1999)
- Schmidt DG, "Raising the pH of the pepsin-catalysed hydrolysis of bovine whey proteins increases the antigenicity of the hydrolysates", Clin Exp Allergy, 25, pp.1007~1017 (1995)
- van Esch BC, "In vivo and in vitro evaluation of the residual allergenicity of partially hydrolysed infant formulas", Toxicol Lett, 201, pp.264~269 (2011)
- Docena G, "Evaluation of the residual antigenicity and allergenicity of cow's milk substitutes by in vitro tests", Allergy, 57, pp.83~91 (2002)
- Restani P, "Evaluation by SDS-PAGE and immunoblotting of residual antigenicity in hydrolysed protein formulas", Clin Exp Allergy, 25, pp.651~658 (1995)