PFG NMR Study of Intra-cellular Drug Uptake in Xenopus laevis Oocyte
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Abstract
Intra-cellular drug uptake in Xenopus laevis oocyte has been elucidated using localized MR spectroscopy (MRS) and PFG NMR techniques at a 600 MHz (Bruker, 14.1 T) NMR spectrometer. The localized MRS has been done with a home-made probe, and shows the intra-cellular uptake of nicotinamide. The self-diffusion of the molecule in Xenopus oocyte was obtained by PFG NMR technique. The measured data are well fitted with a linear combination of two exponential functions, which shows that there are two types of drug molecules, intra- and extra-cellular molecules. Diffusion coefficients of intra- and extra-cellular drug molecules are 3.7 × 10-11 m2/s and 6.4 × 10-10 m2/s, respectively. In the weighting factors there is shown that about 5% of drug molecule is inside the cells. These techniques can be used for drug screening in molecule-, cell-, and tissue-based preclinical test.
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