NMR Study on the Preferential Binding of the Zα Domain of Human ADAR1 to CG-repeat DNA Duplex
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The Z-DNA domain of human ADAR1 (ZαADAR1) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region.9 However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of ZαADAR1. Thus, we have performed NMR experiments of ZαADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that ZαADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.
Abstract
The Z-DNA domain of human ADAR1 (ZαADAR1) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region.9 However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of ZαADAR1. Thus, we have performed NMR experiments of ZαADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that ZαADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.
참고문헌 (15)
- A. Herbert, , J. Biol. Chem, 271, pp.11595~ (1996)
- A. Herbert, , Genetica, 106, pp.37~ (1999)
- A. Rich, , Nat. Rev. Genet, 4, pp.566~ (2003)
- T. Schwartz, , Science, 284, pp.1841~ (1999)
- T. Schwartz, , Nat. Struct. Biol, 8, pp.761~ (2001)
- S. C. Ha, , Proc. Natl. Acad. Sci. USA, 101, pp.14367~ (2004)
- D. Kim, , Nucleic Acids Res, 42, pp.5937~ (2014)
- S. C. Ha, , Nature, 437, pp.1183~ (2005)
- Y. -M. Lee, , J. Am. Chem. Soc, 134, pp.5276~ (2012)
- Y. -M. Lee, , Biophys Chem, 172, pp.18~ (2013)
- Y. -M. Kang, , J. Am. Chem. Soc, 131, pp.11485~ (2009)
- J-. H. Lee, , Nucleic Acids Res, 35, pp.2965~ (2007)
- Y. -J. Seo, , FEBS Lett, 584, pp.4344~ (2010)
- 이준화, "Temperature dependent hydrogen exchange study of DNA duplexes containing binding sites for Arabidopsis TCP transcription factors", Journal of the Korean Magnetic Resonance Society, 18(2), pp.52~57 (2014), 한국자기공명학회[DOI]
- 최용근, "NMR Study of Consensus DNA-binding Site for Arabidopsisthaliana ClassI Transcription Factor AtTCP1", Journal of the Korean Magnetic Resonance Society, 17(2), pp.76~80 (2013), 한국자기공명학회