Backbone assignment and structural analysis of anti-CRISPR AcrIF7 from Pseudomonas aeruginosa prophages
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초록
The CRISPR-Cas system provides adaptive immunity for bacteria and archaea against invading phages and foreign plasmids. In the Class 1 CRISPR-Cas system, multi-subunit Cas proteins assemble with crRNA to bind to DNA targets. To disarm the bacterial defense system, bacteriophages evolved anti-CRISPR (Acr) proteins that actively inhibit the host CRISPR-Cas function. Here we report the backbone resonance assignments of AcrIF7 protein that inhibits the type I-F CRISPR-Cas system of Pseudomonas aeruginosa using triple-resonance nuclear magnetic resonance spectroscopy. We employed various computational methods to predict the structure and binding interface of AcrIF7, and assessed the model with experimental data. AcrIF7 binds to Cas8f protein via flexible loop regions to inhibit target DNA binding, suggesting that conformational heterogeneity is important for the Cas–Acr interaction.
Abstract
The CRISPR-Cas system provides adaptive immunity for bacteria and archaea against invading phages and foreign plasmids. In the Class 1 CRISPR-Cas system, multi-subunit Cas proteins assemble with crRNA to bind to DNA targets. To disarm the bacterial defense system, bacteriophages evolved anti-CRISPR (Acr) proteins that actively inhibit the host CRISPR-Cas function. Here we report the backbone resonance assignments of AcrIF7 protein that inhibits the type I-F CRISPR-Cas system of Pseudomonas aeruginosa using triple-resonance nuclear magnetic resonance spectroscopy. We employed various computational methods to predict the structure and binding interface of AcrIF7, and assessed the model with experimental data. AcrIF7 binds to Cas8f protein via flexible loop regions to inhibit target DNA binding, suggesting that conformational heterogeneity is important for the Cas–Acr interaction.
참고문헌 (25)
- R. Jansen, , Mol. Microbiol., 43, pp.1565~ (2002)
- R. Barrangou, , Science, 315, pp.1709~ (2007)
- S. J. Brouns, , Science, 321, pp.960~ (2008)
- E. V. Koonin, , Curr. Opin. Microbiol., 37, pp.67~ (2017)
- K. S. Makarova, , Nat. Rev. Microbiol., 13, pp.722~ (2015)
- J. E. Samson, , Nat. Rev. Microbiol., 11, pp.675~ (2013)
- A. R. Davidson, , Annu. Rev. Biochem., 89, pp.309~ (2020)
- T. Wiegand, , Annu. Rev. Microbiol., 74, pp.21~ (2020)
- N. Jia, , Nat. Rev. Mol. Cell Biol., 22, pp.563~ (2021)
- A. Pawluk, , Nat. Microbiol., 1, pp.1~ (2016)
- I. Kim, , Nucleic Acids Res., 48, pp.9959~ (2020)
- C. Gabel, , Nucleic Acids Res., 49, pp.584~ (2021)
- F. Delaglio, , J. Biomol. NMR, 6, pp.277~ (1995)
- B. A. Johnson, , J. Biomol. NMR, 4, pp.603~ (1994)
- W. Lee, , Bioinformatics, 31, pp.1325~ (2015)
- Y. Shen, , Proc. Natl. Acad. Sci. USA, 105, pp.4685~ (2008)
- O. F. Lange, , Proc. Natl. Acad. Sci. USA, 109, pp.10873~ (2012)
- Y. Shen, , J. Biomol. NMR, 44, pp.213~ (2009)
- M. V. Berjanskii, , J. Am. Chem. Soc., 127, pp.14970~ (2005)
- 김보경, "Backbone NMR chemical shift assignment of transthyretin", Journal of the Korean Magnetic Resonance Society, 25(1), pp.8~11 (2021), 한국자기공명학회[DOI]
- 강동훈, "Backbone NMR assignments of the FAS1-3", FAS1-4 domains of transforming growth factor-beta-induced protein, 2020, pp.24(1), 1~8[DOI]
- I. Kim, , Sci. Rep., 8, pp.1~ (2018)
- 김익태, "1H, 15N, and 13C Resonance Assignments of the Anti-CRISPR AcrIIA4 from Listeria monocytogenes Prophages", Journal of the Korean Magnetic Resonance Society, 22(3), pp.71~75 (2018), 한국자기공명학회[DOI]
- S. Neal, , J. Biomol. NMR, 26, pp.215~ (2003)
- Y. Shen, , J. Biomol. NMR, 38, pp.289~ (2007)