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Stable Isotope Labeling of Proteins in Mammalian Cells

이정호|KyungRyun Lee
한국자기공명학회 (Korean Magnetic Resonance Society)|2020.09|Journal of the Korean Magnetic Resonance Society|제24권 제3호|pp. 77-85|DOI 10.6564/JKMRS.2020.24.3.077

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참고문헌

키워드

Mammalian expressionstable isotope labelingIn cell NMR
주제분류화학

초록

Stable isotope enrichment in proteins is necessary for high-resolution nuclear magnetic resonance (NMR) experiments. Although methods for 13C, 15N and 2H-enrichment in prokaryotic cells are well established, full processing and correct folding of complex protein systems require higher organisms as the expression host. In the present study, we review recent efforts to enrich stable isotopes in mammalian cells for protein NMR studies.

Abstract

Stable isotope enrichment in proteins is necessary for high-resolution nuclear magnetic resonance (NMR) experiments. Although methods for 13C, 15N and 2H-enrichment in prokaryotic cells are well established, full processing and correct folding of complex protein systems require higher organisms as the expression host. In the present study, we review recent efforts to enrich stable isotopes in mammalian cells for protein NMR studies.

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관련 논문AI추천

NMR Study of larger proteins using isotope labeling박성진한국자기공명학회 · Journal of the Korean Magnetic Resonance Society2014 · 제18권 제2호Cost-effective isotope labeling technique developed for ^(15)N/^(13)C-labeled proteins김희연, 이원태, Eunmi Hong한국자기공명학회 · Journal of the Korean Magnetic Resonance Society2011 · 제15권 제2호In-cell nuclear magnetic resonance spectroscopy for studying intermolecular interactions이영호, Toshihiko Sugiki, Yuxi Lin한국자기공명학회 · Journal of the Korean Magnetic Resonance Society2019 · 제23권 제1호Practical Guide to NMR-based Metabolomics - I : Introduction and Experiments정영상한국자기공명학회 · Journal of the Korean Magnetic Resonance Society2017 · 제21권 제3호Tracing metabolic flux in vivo: basic model structures of tracer methodologyKim Hee Joo, Kim Il-Young, Kim Yeongmin, Park Sanghee, Wolfe Robert R.생화학분자생물학회 · Experimental and Molecular Medicine2022 · 제54권 Validation of protein refolding via 1-dimensional 1H-15N heteronuclear single quantum correlation experiments류경석, 최준혁, Boram Kim한국자기공명학회 · Journal of the Korean Magnetic Resonance Society2019 · 제23권 제4호

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