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半夏가 스트레스로 인한 생쥐의 뇌조직 유전자변화에 미치는 영향 연구

Genome Wide Expression Analysis of the Effect of Pinelliae Rhizoma Extract on Psychological Stress

김하나|김경옥|조수인|송영길|정종효
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2015.03|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제26권 제1호|pp. 63-78

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키워드

Psychological StressMicroarray.Pinelliae Rhizoma Extract (PRe)
주제분류한의학

초록

Objectives: Pinelliae Rhizoma has traditionally been used as an anti-depressant in oriental medicine. This study is to investigate the effect of Pinelliae Rhizoma extract (PRe) on psychological stress in ge-nome wild expression of mice.Methods: After giving physical stress to mice, PRe was orally administered with 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, their genome changes were observed by mi-corarray analysis method. The genome changes were analyzed by IMAGENE 4.0, TREEVIEW, FatiGo al-gorithems, BOND database, cytoscape program, etc.Results: 1. PRe administered group were remained at normal level; 60% of increase was shown in ex-pressed genes by physical stress, and 65% of decrease was shown in expressed genes by psychological stress. 2. Genes with increased expression in control group that remained at a normal state in PRe ad-ministered group were involved with the gene of a cellular metabolic process on biological process, protein binding on molecular function, and cell part on cell composition. The pathway was found to be cytokin-cytokin receptor interaction. 3. Genes with decreased expression in control group that re-mained at a normal state in PRe administered group were involved with the gene of a cellular metabol-ic process on biologiacl detail and coupled ATPaes activity on molecular function. This gene related path was Ubiquintin mediated proteolysis etc. 4. Core node genes analyzed by protein interaction net-work were Vinculin, Cell sdivision cycle 42 homolog (S. cerevisiae) etc. They played an important role in maintaining cytoskeleton and controlling cell cycle.Conclusions: Several genes were up-regulated and down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice. When the interaction network information was ana-lyzed, the recovery of the core node genes in PRe treated mice indicates that this final set of genes may be the effective target of PRe.

Abstract

Objectives: Pinelliae Rhizoma has traditionally been used as an anti-depressant in oriental medicine. This study is to investigate the effect of Pinelliae Rhizoma extract (PRe) on psychological stress in ge-nome wild expression of mice.Methods: After giving physical stress to mice, PRe was orally administered with 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, their genome changes were observed by mi-corarray analysis method. The genome changes were analyzed by IMAGENE 4.0, TREEVIEW, FatiGo al-gorithems, BOND database, cytoscape program, etc.Results: 1. PRe administered group were remained at normal level; 60% of increase was shown in ex-pressed genes by physical stress, and 65% of decrease was shown in expressed genes by psychological stress. 2. Genes with increased expression in control group that remained at a normal state in PRe ad-ministered group were involved with the gene of a cellular metabolic process on biological process, protein binding on molecular function, and cell part on cell composition. The pathway was found to be cytokin-cytokin receptor interaction. 3. Genes with decreased expression in control group that re-mained at a normal state in PRe administered group were involved with the gene of a cellular metabol-ic process on biologiacl detail and coupled ATPaes activity on molecular function. This gene related path was Ubiquintin mediated proteolysis etc. 4. Core node genes analyzed by protein interaction net-work were Vinculin, Cell sdivision cycle 42 homolog (S. cerevisiae) etc. They played an important role in maintaining cytoskeleton and controlling cell cycle.Conclusions: Several genes were up-regulated and down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice. When the interaction network information was ana-lyzed, the recovery of the core node genes in PRe treated mice indicates that this final set of genes may be the effective target of PRe.

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