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壯元丸加減方인 LMK02가 아밀로이드 前驅蛋白質으로 形質轉換된 초파리에 미치는 효과

Effects of Antidementia on LMK02 in APP-transgenic Fly

김상태|김태헌|강형원|류영수|손형진|한평림|조형권
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2008.07|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제19권 제2호|pp. 151-164

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참고문헌

키워드

drosophilaAPPAlzhemiers disease. LMK02
주제분류한의학

초록

Objective : Recent studies indicate that the deposition of beta-amyloid (Aβ) is related in the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is still not clear. Method : To investigate the potential cellular functions of APP and LMK02, we use transgenic drosophila as a model was treated with either LMK02, and the effect in APP expression was determined by climbing assay. LMK02 have been shown to be neuroprotective in fly model systems. We asked whether dietary supplementation with LMK02 would influence behavior and AD-like pathology in a transgenic fly model. Result : LMK02 water extract have attenuated fly death in vivo. LMK02-treated fly increased percentage of flight ability more longly and survival ratio more than controls. APP-GRIM drosophila treated with LMK02 had significantly less accumulation of APP deposition in the eye and brain as compared to control drosophila. Conclusion : These results suggest that LMK02 prevent APP-induced neurotoxicity through attenuating flies death induced by APP, and may be useful as potential therapeutic agents for AD.

Abstract

Objective : Recent studies indicate that the deposition of beta-amyloid (Aβ) is related in the pathogenesis of Alzheimer's disease (AD), but the underlying mechanism is still not clear. Method : To investigate the potential cellular functions of APP and LMK02, we use transgenic drosophila as a model was treated with either LMK02, and the effect in APP expression was determined by climbing assay. LMK02 have been shown to be neuroprotective in fly model systems. We asked whether dietary supplementation with LMK02 would influence behavior and AD-like pathology in a transgenic fly model. Result : LMK02 water extract have attenuated fly death in vivo. LMK02-treated fly increased percentage of flight ability more longly and survival ratio more than controls. APP-GRIM drosophila treated with LMK02 had significantly less accumulation of APP deposition in the eye and brain as compared to control drosophila. Conclusion : These results suggest that LMK02 prevent APP-induced neurotoxicity through attenuating flies death induced by APP, and may be useful as potential therapeutic agents for AD.

참고문헌 (58)

  1. Lee DY, "The prevalence of dementia in older people in an urban population of Korea. The Seoul study", J Am Geritar Soc, 50, pp.1233~1239 (2002)
  2. 대한신경과학회, "신경학", pp.406~ (2007), 군자출판사
  3. 李符永, "ICD-10 정신 및 행동장애 분류", pp.63~66 (1994), 一潮閣
  4. 김지혁, "동의정신의학", pp.256~271 (1992), 현대의학서적사
  5. Yankner BA, "Mechanism of neuronal degeneration in Alzheimer's disease", Neuron, 16, pp.921~923 (1996)
  6. Alzheimer's disease collaborative group, "The structure of the presenilin 1(SI82) gene and idendification of six novel mutation in early onset AD families", Nat Genet, 11(2), pp.219~222 (1995)
  7. 김승업, "치매. 알츠하이머병", pp.54~80 (1997), 삶과 꿈
  8. Smith, A David, "Biological psychiatry", British Medical Bulletin, 52(30), pp.575~586 (1996)
  9. Selkoe DJ, "Amyloid β-protein and the genetics of Alzheimer's disease", J. Biol. Chem, 271, pp.18295~18298 (1996)
  10. 전국한의과대학 신경정신과 교과서편찬위원회, "한의신경정신과학", pp.327~328 (2007), 집문당
  11. 陳貴延, "實用中西醫結合診斷治療學", pp.824~826 (1990), 中國醫藥科技術出版社
  12. Kaufman D, "Aphasia and related disorders. In Clinical Neurology for Psychiatrists", pp.146~171 (1990), W. B. Saunders
  13. Summers WK, "Oral tetrahydroaminoacridine in long term treatment of senile dementia . Alzheimer type", New Engl. J Med, 315, pp.1241~1245 (1986)
  14. Reisberg B, "Memantine in moderate-to-severe Alzheimer's disease", N Engl. J Med, 348(14), pp.1333~1341 (2003)
  15. 이정현, "치매환자에서 동반된 행동정신증상에 대한 새로운 향정신병약물의 치료효과", 대한정신약물학회지, 11(3), pp.232~237 (2000)
  16. 정영조, "치매의 예방과 관리", 仁濟醫學, 1, pp.11~19 (2000)
  17. 정인철, "山査肉이 Alzheimer's Disease 病態 모델에 미치는 영향", 동의생리병리학회지, 16(2), pp.12~288 (2002), 대한동의병리학회
  18. 이상원, "遠志 디클로로메탄분획이 CT105에 의한 신경세포 傷害에 미치는 영향", 동의생리병리학회지, 18(2), pp.507~516 (2004), 대한동의병리학회
  19. 최혁, "CT105로 유도된 痴呆모델에서 石菖蒲 수추출액이 미치는 영향", 동의신경정신과학회지, 13(2), pp.173~194 (2002)
  20. 장현호, "釣鉤藤 디클로로메탄분획이 CT105에 의한 신경세포 상해에 미치는 영향", 동의생리병리학회지, 18(6), pp.1810~1820 (2004), 대한동의병리학회
  21. 조윤숙, "荊防地黃湯이 Alzheimer's disease 모델 백서의 학습과 기억에 미치는 영향", 동의신경정신과학회지, 9(1), pp.1~24 (1998)
  22. 우주영, "調胃升淸湯이 흰쥐의 방사형 미로 학습과 기억에 미치는 영향", 동의신경정신과학회지, 8(1), pp.69~79 (1997)
  23. 심대식, "육미지황탕가미방에 의한 흰쥐 기억력 향상과 관련된 Hippocampus 부위의 특이 유전자 발현에 대한 연구", 동의생리병리학회지, 17(3), pp.684~692 (2003), 대한동의병리학회
  24. 황선미, 정대규, "공진단이 CT105로 유도된 Alzheimer's disease 병태모델에 미치는 영향", 동의신경정신과학회지, 15(2), pp.103~118 (2004), 대한한방신경정신과학회
  25. 민상연, "補中益氣湯加味方이 흰쥐의 성장과 학습 및 기억에 미치는 영향", 동의생리병리학회지, 19(2), pp.434~440 (2005), 대한동의병리학회
  26. 하수영, "聰明湯과 山査聰明湯이 Alzheimer's Disease 病態 모델에 미치는 影響", 동의신경정신과학회지, 17(1), pp.59~78 (2006), 대한한방신경정신과학회
  27. 이준영, "天王補心丹이 痴呆병태모델에 미치는 영향", 동의신경정신과학회지, 13(2), pp.149~172 (2002)
  28. 김건진, 정대규, "장원단이 CT105와 βA로 誘導된 Alzheimer's Disease 病態 모델에 미치는 影響", 동의신경정신과학회지, 17(2), pp.91~122 (2006), 대한한방신경정신과학회
  29. 강형원, "壯元丸加減方 水抽出物이 아밀로이드 전구단백질으로 유도된 생쥐의 신경아세포주에서의 항치매 효과", 동의신경정신과학회지, 18(2), pp.13~24 (2007), 대한한방신경정신과학회
  30. 許俊, "東醫寶鑑", pp.189~191 (1999), 法仁文化社
  31. Markesbery WR, "Brain trace elements in Alzheimer's disease. In Alzheimer's Disease", neurotoxicology, 7(1), pp.195~206 (1986)
  32. Li WY, "Little SP, Czilli DL, Simmons LK : Suppression of an amyloid beta peptide-mediated calcium channel response by a secreted beta-amyloid precursor protein", Neuroscience, 95(1), pp.1~4 (1999)
  33. Pratico D, "Alzheimer's disease and oxygen radical. new insights", Biochem Pharmachol, 63, pp.563~567 (2002)
  34. Launer LJ, "Rates and risk factors for dementia and Alzheimer’s disease: results from EURODEM pooled analyses. EURODEM Incidence Research Group and Work Groups. EuropeanStudies of Dementia", Neurology, 1, pp.78~84 (1999)
  35. Goate A, "Irving N, James L, et al. : Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease", Nature, 349, pp.704~706 (1991)
  36. Levy-Lahad E, "Candidate gene for the chromosome 1 familial Alzheimer's disease locus", Science, 269, pp.973~977 (1995)
  37. Katzman R, "Apolipoprotein E4 as the genetic susceptibility for Alzheimer's disease. In the Alzheimer's Disease", pp.455~457 (1994), Raven Press
  38. Bachman DL, "Prevalence of dementia and probable senile dementia of the Alzheimer type in the Framingham Study", Neurology, 42, pp.115~119 (1992)
  39. Lai F, "A prospective study of Alzheimer disease in Down syndrome", Arch Neurol, 46, pp.849~853 (1989)
  40. Katzman R, "Epidermiology of dementia and Alzheimer's disease. In the Alzheimer's Disease", pp.105~122 (1994), Raven Press
  41. Steinhoff T, "Increased cystatin c in astrocytes of transgenic mice expressing the K670N-M671L mutation of the amyloid precursor protein and deposition in brain amyloid plaques", Neurobiol Dis, 8(4), pp.647~654 (2001)
  42. De Jonghe C, "Pathogenic APP mutations near the gamma-secretase cleavage site differentially affect Aβ secretion and APP C-terminal fragment stability", Hum Mol Genet, 10(16), pp.1665~1671 (2001)
  43. Fishman CE, "Statistical aspects of quantitative image analysis of beta-amyloid in the APP(V717F) transgenic mouse model of Alzheimer's disease", J Neurosci Methods, 30, pp.145~152 (2001)
  44. Giacobini E, "Cholinesterase inhibitors stabilize Alzheimer’s disease", Ann NY Acad Sci, 920, pp.321~327 (2000)
  45. Kawai M, "Subcellular localization of amyloid precursor protein in senile plaques of Alzheimer's disease", Am J Pathol, 140(4), pp.947~958 (1992)
  46. Giacobini E, "Cholinesterase inhibitors stabilize Alzheimer’s disease", Ann NY Acad Sci, 920, pp.321~327 (2000)
  47. Amenta F, "Treatment of cognitive dysfunction associated with Alzheimer’s disease with cholinergic precursors: Ineffective treatments or inappropriate approaches", Mech Ageing Dev, 122, pp.2025~2040 (2001)
  48. 張介賓, "景岳全書", pp.846~ (1992), 一中社
  49. 陳士鐸, "石室秘錄(下)", pp.316~317 (1984), 書苑堂
  50. 錢鏡湖, "辨證奇文全書", pp.222~225 (1980), 甘地出版社
  51. 정인철, "도담익기활혈탕이 치매병태모델의 생화학적 변화 및 기억에 미치는 영향", 동의생리병리학회지, 15, pp.260~276 (2001)
  52. 방재선, "加味寧神丹이 CT105로 유도된 Neuro2A 세포주에서의 抗痴呆효과", 대한한방내과학회지, 27(3), pp.603~616 (2006)
  53. 김보경, "안신청뇌탕이 치매병태모델의 생화학적 변화 및 기억에 미치는 영향", 동의생리병리학회지, 16, pp.240~255 (2006)
  54. 박지운, "日黃連이 痴呆병태모델에 미치는 영향", 동의신경정신과학회지, 15(1), pp.87~100 (2004)
  55. 이성률, "遠志와 石菖蒲 혼합추출액의 pCT105로 유도된 신경세포암 세포주에 대한 抗痴呆효과", 동의생리병리학회지, 17(4), pp.1037~1049 (2003)
  56. Iijima K, "Dissecting the pathological effects of human Aβ40 and Aβ42 in Drosophila: a potential model for Alzheimer's disease", Proc Natl Acad Sci USA, 통권 : 101, pp.6623~6628 (2004)
  57. Finelli A, "A model for studying Alzheimer's Aβ42-induced toxicity in Drosophila melanogaster", Mol Cell Neurosci, 26, pp.365~375 (2004)
  58. 龔廷賢, "增補萬病回春", pp.229~230 (1994), 一中社

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