五子地黃飮子 열수추출물과 초미세분말이 싸이토카인과 건망증 생쥐모델 기억력감퇴에 미치는 영향
The Effects of OJaJiHwangEumJa(OJJHEJ) Hot water extract & Ultra-fine Powder on Proinflammatory Cytokine of Microglia and Memory Deficit Model
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Background : Microglia produces a barrage of factors (IL-1, TNF-α, NO, superoxide) that are toxic to neurons and play a major role in the cellular immune response associated with the pathology of Alzheimer’s disease(AD). OJaJiHwangEumJa(OJJHEJ) has been usually used for the treatment of senile disorders. For enhancing efficacy and convenience, the change of the drug delivery device of oriental herbal medicine is required. Objective : This experiment was designed to investigate the effect of the OJJHEJ hot water extract & ultra-fine powder on proinflammatory cytokine of microglia and memory deficit model. Method : The effects of the OJJHEJ hot water extract on production of IL-1β, IL-6, TNF-α, in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. The effects of the OJJHEJ hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine and AChE in serum of the memory deficit mice induced by scopolamine were investigated. Results : 1. The OJJHEJ hot water extract suppressed the production of IL-1β, IL-6, TNF-α in BV2 microglial cell line and the production of IL-6 was suppressed significantly. 2. The OJJHEJ hot water extract & ultra-fine powder decreased AChE significantly in the serum of the memory deficit mice induced by scopolamine. 3. The OJJHEJ hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. Conclusions : This experiment shows that the OJJHEJ hot water extract & ultra-fine powder might be effective for the prevention and treatment of memory impairment diseases. Investigation into the clinical use of the OJJHEJ hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.
Abstract
Background : Microglia produces a barrage of factors (IL-1, TNF-α, NO, superoxide) that are toxic to neurons and play a major role in the cellular immune response associated with the pathology of Alzheimer’s disease(AD). OJaJiHwangEumJa(OJJHEJ) has been usually used for the treatment of senile disorders. For enhancing efficacy and convenience, the change of the drug delivery device of oriental herbal medicine is required. Objective : This experiment was designed to investigate the effect of the OJJHEJ hot water extract & ultra-fine powder on proinflammatory cytokine of microglia and memory deficit model. Method : The effects of the OJJHEJ hot water extract on production of IL-1β, IL-6, TNF-α, in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. The effects of the OJJHEJ hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine and AChE in serum of the memory deficit mice induced by scopolamine were investigated. Results : 1. The OJJHEJ hot water extract suppressed the production of IL-1β, IL-6, TNF-α in BV2 microglial cell line and the production of IL-6 was suppressed significantly. 2. The OJJHEJ hot water extract & ultra-fine powder decreased AChE significantly in the serum of the memory deficit mice induced by scopolamine. 3. The OJJHEJ hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. Conclusions : This experiment shows that the OJJHEJ hot water extract & ultra-fine powder might be effective for the prevention and treatment of memory impairment diseases. Investigation into the clinical use of the OJJHEJ hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.
참고문헌 (42)
- 민성길, "최신정신의학", pp.205~ (2006), 일조각
- 조추용, "치매 예방과 케어", pp.49~ (2002), 창지사
- Cacquevel M, "Cytokines in neuroinflammation and Alzheimer's disease", Current Drug Targets, 5(6), pp.529~534 (2004)
- Kuhl DE, "In vivo mapping of cerebral acetylcholinesterase activity in aging and Alzheimer's disease", Neurology, 52(4), pp.691~699 (1999)
- Mhatre M, "Oxidative stress and neuroinflammation in Alzheimer's disease and amyotrophic lateral sclerosis: common links and potential therapeutic targets", Journal of Alzheimers disease, 6(2), pp.147~157 (2006)
- 안준익, "Beta-amyloid의 분자생물학", 한양의대학술지, 21(1), pp.11~16 (2001)
- 張介賓, "張氏景岳全書", pp.610~611 (1978), 翰成社
- 錢鏡湖, "辨證奇問全書", pp.222~225 (1990), 甘地出版社
- 洪元植, "精校黃帝內經素問", pp.37~ (1985), 東洋醫學硏究院
- 대한한방신경정신과학회, "한방신경정신과학", pp.311~320 (2005), 집문당
- 유완소, "선명론방", pp.150~ (1990), 도서출판정담
- 李尙仁, "본초학", pp.117~119 (1981), 학림사
- 대전대학교 한방병원, "대전대학교 한방병원 처방집", pp.120~ (1992), 한국 출판사
- 정인철, "山査肉이 CT-105로 誘導된 Alzheimer's Disease 病態 모델에 미치는 影響", 동의생리병리학회지, 16(2), pp.279~288 (2002)
- 박지운, "日黃連이 치매병태모델에 미치는 영향", 동의신경정신과학회지, 15(1), pp.87~100 (2004)
- 최병만, 이상룡, 정인철, "목근피가 CT105와 Ba로 유도된 Alzheimer's Disease 병태모델에 미치는 영향", 동의신경정신과학회지, 15(2), pp.119~139 (2004), 대한한방신경정신과학회
- 김명진, 정인철, 이상룡, "木瓜가 CT105와 βA로 誘導된Alzheimer's Disease病態 모델에 미치는 影響", 동의신경정신과학회지, 16(1), pp.97~117 (2005), 대한한방신경정신과학회
- 서경석, "오자지황음자가 노화백서의 혈액 변화와 혈청, 뇌조직의 항산화활성에 미치는 영향", 동의신경정신과학회지, 10(1), pp.79~93 (1999)
- 최현정, "한약제형 선호도에 관한 설문조사", 경희의학, 20(1), pp.356~367 (2004)
- Shu ZH, "Study on the superfine comminution of traditional Chinese medicine", Zhongguo Zhong Yao Za Zhi, 29(9), pp.823~827 (2004)
- Skehan P, "New colorimetric cytotoxicity assay for anticancer drug screening", Journal of the National Cancer Institute, 82(13), pp.1107~1112 (1990)
- 홍은경, "치매의 신경병리학", 한양의대학술지, 21(1), pp.1~9 (2001)
- Bierer L. M, "Neurochemical correlates of dementia severity in Alzheimer's disease: relative importance of the cholinergic deficits", Journal of Neurochemistry, 64, pp.749~760 (1995)
- Hunot S, "FcepsilonRII", CD23 is expressed in Parkinson's disease and induces, in vitro, production of nitric oxide and tumor necrosis factor-alpha in glial cells, 1999, pp.19(9), 3440~3447
- Trabace L, "Biochemical and neurobehavioral profile of CHF2819, a novel, orally active acetylcholinesterase inhibitor for Alzheimer's disease", The Journal of Pharmacology and Experimental Therapeutics, 294(1), pp.187~194 (2000)
- Kasa P, "Donepezil dose-dependently inhibits acetylcholinesterase activity in various areas and in the presynaptic cholinergic and the postsynaptic cholinoceptive enzyme-positive structures in the human and rat brain", Neuroscience, 101(1), pp.89~100 (2000)
- Geerts H, "Indicators of neuroprotection with galantamine", Brain Research Bulletin, 64(60), pp.519~524 (2005)
- Spassov A, "Study of ancholinesterase drugs galanthamine and donepezil on scopolamine-induced amnesia in rats", European Neuropsychopharmacology, 14, pp.333~ (2004)
- Reznik-Wolf H, "Somatic mutation analysis of the APP and Presenilin 1 and 2 genes in Alzheimer's disease brains", Journal of Neurogenetics, 12(1), pp.55~65 (1998)
- Mattson MP, "Secreted form of amyloid precursor protein enhances basal glucose and glutamate transport and protects against oxidative impairment of glucose and glutamate transport in synaptosomes by a cyclic GMP-mediated mechanism", Journal of Neurochemistry, 73(2), pp.532~537 (1999)
- 陳士鐸, "石室秘錄", pp.125~ (1991), 中國中醫藥出版社
- 錢鏡湖, "辨證奇問全書", pp.222~225 (1990), 甘地出版社
- 洪元植, "精校黃帝內經素問", pp.217~218 (1985), 東洋醫學硏究院
- 朱震亨, "金櫃鉤玄", pp.306~307 (1992), 鼎談出版社
- 황도연, "증맥 방약합편", pp.121~ (1989), 남산당
- 리정복, "장수학", pp.41~ (1989), 과학백과사전출판사
- Lee SC, "Cytokine production by human fetal microglia and astrocyte", Journal of Immunology, 150, pp.2659~ (1998)
- Nick J, "Activation of p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide", Journal of Immunology, 156, pp.4457~ (1996)
- Woo MS, "Selective modulation of lipopolysaccharide-stimulated cytokine expression and mitogen-activated protein kinase pathways by dibutyryl-cAMP in BV2 microglial cells", Molecular Brain Research, 113(1), pp.86~96 (2003)
- Tuppo E, "The role of inflammation in Alzheimer's disease", The International Journal of Biochemistry&Cell Biology, 37(2), pp.289~305 (2005)
- Akama KT, "Cerebrospinal fluid S100B is elevated in the earlier stages of Alzheimer's disease", Neurochemistry International, 39(5), pp.409~413 (2001)
- Ebert U, "Scopolamine model of dementia ; electroencephalogram findings and cognitive performance", European Journal of Clinical Investigation, 28(11), pp.944~949 (1998)