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급성 알코올 중독 어린 흰쥐의 해마 치상회에서 용담추출물이 신경세포생성과 세포사멸에 미치는 영향

Effect of Gentianae Radix on Neurogenesis and Apoptosis in Hippocampus of Ethanol-induced Newborn Rats

이재혁|김이화|이진규
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2010.06|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제21권 제2호|pp. 29-44

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키워드

ApoptosisAlcoholHippocampusNeurogenesisGentianae RadixDentate Gyrus
주제분류한의학

초록

Objectives :The purpose of this study was to investigate the effect of Gentianae Radix on neurogenesis and apoptosis in ethanol- induced newborn rats hippocampus dentate gyrus. Methods :In vivo, laboratory animals were divided into three groups; Normal group(N), Control group(C) and Treated group (TG)(n=7 for each group). N were treated saline daily for five days. C were treated 1.5 g/㎏ ethanol and saline daily for five days. TG were treated 1.5 g/㎏ ethanol and 300 ㎎/㎏ Gentianae Radix daily for five days. BrdU(5-bromo-2-deoxyuridine) assay was used to test neurogenesis in the dentate gyrus. And TUNEL(Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay was used to test apoptosis in the dentate gyrus. Three groups were measured body weight, serum ethanol concentration, BrdU-positive cells and TUNEL-positive cells in the dentate gyrus. In vitro, MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to test viability in SK-N-MC cells. BrdU assay was used to test neurogenesis in SK-N-MC cells. DNA fragmentation and caspase-3 enzyme activity assay were used to test apoptosis in SK-N-MC cells. And treated ethanol and Gentianae Radix of all in vitro tests were made various concentration. Results :In vivo, Gentianae Radix modulated ethanol-induced neurogenesis and apoptosis in newborn rats hippocampus dentate gyrus. In vitro, TG 100 ㎍/㎖ have significantly modulated ethanol-induced neurogenesis and apoptosis in SK-N-MC cells. And only TG 100 ㎍/㎖ have significantly protected SK-N-MC cells from ethanol-induced cytotoxicity. Conclusions :Gentianae Radix may have the effect that modulated ethanol-induced neurogenesis and apoptosis in SK-N-MC cells.

Abstract

Objectives :The purpose of this study was to investigate the effect of Gentianae Radix on neurogenesis and apoptosis in ethanol- induced newborn rats hippocampus dentate gyrus. Methods :In vivo, laboratory animals were divided into three groups; Normal group(N), Control group(C) and Treated group (TG)(n=7 for each group). N were treated saline daily for five days. C were treated 1.5 g/㎏ ethanol and saline daily for five days. TG were treated 1.5 g/㎏ ethanol and 300 ㎎/㎏ Gentianae Radix daily for five days. BrdU(5-bromo-2-deoxyuridine) assay was used to test neurogenesis in the dentate gyrus. And TUNEL(Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay was used to test apoptosis in the dentate gyrus. Three groups were measured body weight, serum ethanol concentration, BrdU-positive cells and TUNEL-positive cells in the dentate gyrus. In vitro, MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to test viability in SK-N-MC cells. BrdU assay was used to test neurogenesis in SK-N-MC cells. DNA fragmentation and caspase-3 enzyme activity assay were used to test apoptosis in SK-N-MC cells. And treated ethanol and Gentianae Radix of all in vitro tests were made various concentration. Results :In vivo, Gentianae Radix modulated ethanol-induced neurogenesis and apoptosis in newborn rats hippocampus dentate gyrus. In vitro, TG 100 ㎍/㎖ have significantly modulated ethanol-induced neurogenesis and apoptosis in SK-N-MC cells. And only TG 100 ㎍/㎖ have significantly protected SK-N-MC cells from ethanol-induced cytotoxicity. Conclusions :Gentianae Radix may have the effect that modulated ethanol-induced neurogenesis and apoptosis in SK-N-MC cells.

참고문헌 (45)

  1. 민성길, "최신정신의학", pp.32~450 (2006), 일조각
  2. John Kerr, "Apoptosis: Its significance in cancer and cancer therapy", Cancer, 73(8), pp.2013~2026 (2006)
  3. Bergman H, "Computed-tomography of the brain and neuropsychological asscssement of alcoholic patients", Advances in ExperimentaI Medicine and Biology, 126, pp.771~786 (1980)
  4. Fulton Crews, "Effects of ethanol on ion channels", International Review of Neurobiology, 39, pp.283~367 (1996)
  5. Alling C, "Evaluation of ethanol effects on PLC. signal transduction patlnvays using cell lines of neuronal origin", Alcohol and Alcoholism, 2, pp.2295~299 (1993)
  6. Daniel Bonthius, "Reduced seizure threshold and hippocampal cell loss in rats exposed to alcohol during the brain growth Spurlt", Alcoholism Clinical and Experimental Research, 25(1), pp.70~82 (2001)
  7. 권재철, "급속한 알코올 투여 후 흰쥐 뇌의 유전자 말현변화 분석", 경상대학교 대학원, ~ (2003)
  8. 유병규, "흰쥐 해마의 치상회에서 압박자극 작용이 뇌 신경세포 증식에 미치는 영향", 대한감각통합치료학회지, 2(1), pp.1~12 (2004)
  9. 김현중, "對金飮子 약침이 알코올 독성 흰쥐의 해마에서 신경세포생성과 NOS 발현에 미치는 영향", 대한침구학회지, 23(5), pp.187~198 (2006), 대한침구학회
  10. 신민철, "인삼이 흰쥐의 치상회에서 알코올에 의한 새로운 신경세포 생성 및 nitric oxide synthase 발현에 미치는 영향", 대한한의학회지, 23(3), pp.444~450 (2002), 대한한의학회
  11. 한경승, "해주기갈탕, 단주환 잊 성주청간탕이 실험적 Alcoholism에 미치는 영향", 경희대학교 대학원, ~ (1992)
  12. 곽익훈, "淸肝散이 急 性 Ethanol 中毒에 흰쥐의 肝損傷에 마치는 影響", 한국한의학연구원논문집, 2(1), pp.177~191 (1996)
  13. 공경환, "Alcohol, Indomethacin 및 Burn-stress로 유발된 생쥐의 위점막 손상에 대한 대금음자, 익위탕, 시호소간산의 효과", 대한한의학회지, 28(2), pp.166~184 (2007), 대한한의학회
  14. 전국한의과대학 신경정신과 교과서편찬위원회, "한의신경정신과학", pp.35~345 (2007), 집문당
  15. 李東垣, "東垣醫集", pp.117~123 (1996), 人民衛生出版社
  16. 중약대사전 편찬위원회, "중약대사전", pp.3176~3181 (1997), 정당
  17. 강병수, "본초학", pp.184~185 (2000), 영림사
  18. 김미선, "龍膽草 추출물이 LPS로 활성화된 Raw 264.7 cell에서의 pro-inflammatory mediator에 미치는 영향", 한방안이비인후피부과학회지, 21(2), pp.28~38 (2008), 대한한방안이비인후피부과학회
  19. 박동석, "농도별 초룡담(草龍 膽) 수침이 담석 유발 생쥐에 미치는 영향", 대한침구학회지, 8(1), pp.141~156 (1991)
  20. 황의완, "동의정신의학", pp.427~441 (1987), 서울현대의학 서적사
  21. 우홍정, "간계내과학", pp.301~314 (2001), 동양의학연구원출판사
  22. 김세훈, "에타놀 정맥투여 고양이의 뇌압 변화에 관한 연구", 충남의대잡지, 14(2), pp.71~78 (1987)
  23. 배병철, "黃帝內經讀解", pp.213~658 (2000), 성보사
  24. 許俊, "東醫寶鑑", pp.1135~1139 (1999), 법인문화사
  25. 김용훈, "精神機能所在로서의 뇌에 대한 한의학적 해석근거 연구", 동의생리병리학회지, 16(5), pp.8~887 (2002), 대한동의병리학회
  26. 오은기, "알코올 농도에 따른 주상치료 처방의 선택에 관한 문헌 연구:동의보감 수록 처방을 중심으로", 상지대학교 대학원, ~ (2008)
  27. 김주곤, "韓茵蔯蒿湯이 알코올 투여로 유발된 흰쥐의 고지혈증과 간 손상의 예방에 미치는 영향", 대한본초학회지, 20(1), pp.9~18 (2005), 대한본초학회
  28. Michael Aminoff, "Neurology and General Medicine", pp.617~629 (2001), Churchill Livingstone
  29. Paul Perry, "The association of alcohol-induced blackouts and grayouts to blood alcohol concentrations", Journal of Forensic Sciences, 51(4), pp.896~899 (2006)
  30. Aaron White, "What happered? Alcohol, Memory blackouts, and the brain", Alcohol Research and Health, 27, pp.186~196 (2003)
  31. Judson Chandler, "Chonic ethanol exposure potentiatcs NMDA excitotoxicity in cerebral cortical neurons", Journal of Neurochemistry, 60(4), pp.1578~1581 (1993)
  32. 장형서, "알코올이 미성숙 생쥐 뇌에 미치는 영향", 한양대학교, ~ (2007)
  33. Frank Netter, "CIBA 원색도해의학총서-Nervous System I", pp.27~28 (2000), 정담
  34. Joseph Altman, "Autoradiographic and histological evidence of postnataI hippocampal neurogenesis in rats", The Joumal of Comparative Neurology, 124, pp.319~335 (1965)
  35. Maryellen Eckenhoff, "Nature and fate of proliferative cells in the hippocampal dentate gyTUS during tilC life span of the rhesus monkey", Journal of Neuroscience, 8(8), pp.2729~2747 (1988)
  36. Fred Gage, "Theo Palmer, Daniel Peterson, Jasodhara Ray. Multipotent progenitor cells in the adult dentate gyrus", Journal of Neurobiology, 36, pp.249~266 (1998)
  37. 서지원, "용담초 추출물의 분리와 항산화 효과" (2008), 원광대학교 대학원
  38. 한상원, "용담초(龍膽草)및 사호수침(柴胡水鍼) 이 CCI_4 유발된 흰쥐의 손상간에 미치는 영향", 대한침구학회지, 10(1), pp.297~313 (1993)
  39. 이민정, "알코올을 섭취한 흰쥐에 있어서 생맥산이 간 보호작용과 지질대사에 미치는 영향", 건국대학교 대학원, ~ (2001)
  40. 최현숙, "달래가 에탄올을 투여한 흰쥐의 간 독성에 미치는 항산화 효과", 조선대학교 대학원, ~ (2002)
  41. 이은경, "랫드의 알코올성 간 손상에 대한 2- Methylaminoethyl-4,4' -dimethoxy-5,6,5',6'-di methy lenedioxybipheny1-2-carboxylic acid-2'-carboxylate monohydrohloride 의 보호효과", 부산대학교 대학원, ~ (2004)
  42. Nicholas Schisler, , Free Radical Biology and Medicine, 7(2), pp.117~123 (1989)
  43. N. Kee, "The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis", Joumal of Neuroscience Methods, 115(1), pp.97~105 (2002)
  44. Francoise Labat-Moleur, "TUNEL apoptotic cell detection in tissue sections: critical evaluation and improvement", Journal of Histochemistry and Cytochemistry, 46, pp.327~334 (1998)
  45. Robert Ross, "Coordinate morphological and biochemical intcrconversion of human neuroblastoma cells", Journal of the National Cancer Institute, 71(4), pp.741~747 (1983)

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