흰쥐의 적혈구 내 항 산화효소에 대한 인 성장호르몬의 효과
Effect of Human Growth Hormone to Antioxidant Enzymes in Rat Erythrocyte
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목적: 본 연구는 뇌하수체절제된 백서에서 성장호르몬의 투여 용량에 따른 혈장 내 MDA (malondialdehyde), 적혈구 내 효소인 SOD (superoxide dismutase), catalase 간의 연관성을 알아보고자 시행하였다. 방법: 40마리의 백서를 4군으로 나누어 뇌하수체절제하여 2주간 키운 뒤 A군은 성장호르몬을 투여하지 않고, 나머지 3군들은 성장호르몬을 1, 4, 8 IU/kg/day (B, C, D군)씩 4주간 경피주사하였다. 4군으로부터 혈액을 채취하여 MDA, catalase, SOD를 측정하였다. 결과: C, D군에서 성장호르몬 투여 용량과 catalase와 SOD의 활성도 간에 유의한 차이가 관찰되었다. 성장호르몬을 투여했던 군에서 catalase와 SOD의 활성도는 감소한 반면 모든 군에서 MDA 간의 유의한 차이는 없었다. 결론: 이번 실험 결과는 catalase와 SOD의 활성도가 성장호르몬과 연관성이 있음을 암시한다. 이는 성장호르몬의 친 산화 또는 항산화작용을 설명할 수 있는 실마리가 될 수도 있을 것이다. 앞으로 성장호르몬에 의해 유발되는 혈장 내 지질 산화의 영향을 알아보기 위하여 더 많은 연구가 이루어져야 할 것이다.
Abstract
Objectives: To observe whether any relationship exists between the dosage of growth hormone and the plasma concentration of malondialdehyde (MDA) or whether a relationship exists between the dosage of growth hormone and the activity of erythrocyte enzymes, superoxide dismutase (SOD) and catalase, on hypophysectomized male rats. Methods: We used 40 hypophysectomized Sprague-Dewley rats randomly assigned to four groups. The first group (group A) was allowed to evolve freely with no growth hormone supply. Human growth hormone was administered subcutaneously to the other three groups at dose of 1 IU/kg/day (group B), 4 IU/kg/day (group C) and 8 IU/kg/day (group D) after 2 weeks of recovery date from operation and 4 weeks of growth hormone treatment, blood samples were obtained from the four groups. The concentrations of MDA, the concentrations of erythrocyte catalase and SOD were determined. Results: There were significant correlations between the dose of growth hormone and the concentrations of erythrocyte catalase and SOD in the group C,D Whereas catalase and SOD activities in erythrocyte was decreased in growth hormone group. Any relationship wasn't found between the growth hormone and plasma MDA levels in all groups. Conclusion: These results suggested that the catalase and SOD in erythrocyte is related the dosage of growth hormone. This model may explain the contradictory findings presented by growth hormone with respect to their pro- or antioxidant action. The further study will be needed to evaluate the effect of growth hormone induced plasma lipid peroxidation.
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