세신 정유추출물의 목재부후균과 궐련벌레에 대한 항진균 및 살충활성
Antifungal and Insecticidal Activity of Essential Oil from Asarum sieboldii against Wood Contaminant Fungi and Lasioderma serricorne L.
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초록
정유 상태의 세신(Asarum sieboldii) 추출물이 유기질 문화재 보존을 위한 천연 살생물제로써의 적합성 여부를 탐색하기 위하여 목재부후균과 궐련벌레에 대한 항진균활성 및 살충활성을 조사하였다. 목재부후균 4종에 대한 세신 정유추출물의 항진균활성(IC50)을 측정한 결과 1.50~2.84㎕/disc으로 나타났으며, 이 중 L. lepideus에서 활성이 가장우수하였다. 궐련벌레 성충에서는 50%(v/w)과 100%(v/w)의 농도로 처리했을 때 약 90% 이상의 살충활성을 보였다. 가스크로마토그래피-질량분석기를 이용하여 정유추출물의 성분을 분석한 결과 총 35종의 화학성분이 검출되었으며,주성분은 methyleugenol(56.32%), eucarvone(11.53%), safrole(5.79%), δ-3-carene(2.09%) 순으로 나타났다. 따라서 세신 정유추출물은 목재부후균과 궐련벌레 등 곤충에 의해 야기되는 생물학적 열화로부터 유기질 문화재를 보존할 수 있는 천연 살생물제로써 활용 가능성을 확인하였다.
Abstract
To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, essential oil from Asarum sieboldii was screened for antifungal and insecticidal activity against 4 wood rotting fungi and adult of Lasioderma serricorne. Antifungal activity of essential oil was tested by using paper disc soaking method. Antifungal activity expressed as IC50 value showed 1.50~2.84 ㎕/disc range and the most significant antifungal activity was observed in Lentinus lepideus. The insecticidal activity of essential oil was examined by topical application method against L. serricorne adults. 50% and 100% of essential oil gave 98.3% and 100% mortality for 24 hours, respectively. The major components of the essential oil were methyl eugenol (56.32%), eucarvone (11.53%), safrole (5.79%), δ-3-carene (2.09%), which were identified by gas chromatography-mass spectrometry. From these results, essential oil from A. sieboldii could be useful for conservation of organic cultural heritage against biological deterioration by insect and wood rotting fungi.
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