Effects of Endurance Exercise Training on Adipose Tissue Inflammatory Gene Expression in Elderly Rats with Diet-Induced Obesity
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[OBJECTIVES] The study aimed to investigate the effects of the exercise training on inflammatory gene expression in adipose tissue via aging and obesity. [METHODS] The study subjects were forty 10-week old and 70-week old male Wistar rats. The young and oldaged rats were divided into four groups of ten: control (non-exercise) group (Y-Non; O-Non) and exercise group (Y-Ex; O-Ex). After the exercise training, intergroup differences in body composition, oral glucose tolerance test result, and protein expression levels of IL-6, TNFα, IL-10, and IL-4 in adipose tissue, were compared. [RESULTS] Both body weight and body fat-free mass were lower in old-aged rats than young rats; O-Non group, in particular, displayed the highest body fat mass and lowest fat-free mass. In oral glucose tolerance test result, old-aged rats exhibited higher blood glucose and insulin concentrations than young rats. TNF-α protein expression was higher in old-aged rats than young rats with the Y-Ex group displaying the lowest expression. IL-6 protein expression was significantly lower in the exercise group than in the control group for both old-aged and young rats (p < 0.05). IL-10 protein expression was not significantly different between the exercise group and the control group for either old-aged or young rats. IL-4 protein expression was significantly lower in the exercise group of young rats (p < 0.05). [CONCLUSIONS] Based on these findings, the analysis of the effects of exercise training on inflammatory gene expression in the adipose tissue of obese old-aged rats showed that the exercise training has effectively promoted the shift to M2 of cytokines secretion pattern related to obesity-induced pro-inflammation in old-aged rats and helped to improve insulin sensitivity.
Abstract
[OBJECTIVES] The study aimed to investigate the effects of the exercise training on inflammatory gene expression in adipose tissue via aging and obesity. [METHODS] The study subjects were forty 10-week old and 70-week old male Wistar rats. The young and oldaged rats were divided into four groups of ten: control (non-exercise) group (Y-Non; O-Non) and exercise group (Y-Ex; O-Ex). After the exercise training, intergroup differences in body composition, oral glucose tolerance test result, and protein expression levels of IL-6, TNFα, IL-10, and IL-4 in adipose tissue, were compared. [RESULTS] Both body weight and body fat-free mass were lower in old-aged rats than young rats; O-Non group, in particular, displayed the highest body fat mass and lowest fat-free mass. In oral glucose tolerance test result, old-aged rats exhibited higher blood glucose and insulin concentrations than young rats. TNF-α protein expression was higher in old-aged rats than young rats with the Y-Ex group displaying the lowest expression. IL-6 protein expression was significantly lower in the exercise group than in the control group for both old-aged and young rats (p < 0.05). IL-10 protein expression was not significantly different between the exercise group and the control group for either old-aged or young rats. IL-4 protein expression was significantly lower in the exercise group of young rats (p < 0.05). [CONCLUSIONS] Based on these findings, the analysis of the effects of exercise training on inflammatory gene expression in the adipose tissue of obese old-aged rats showed that the exercise training has effectively promoted the shift to M2 of cytokines secretion pattern related to obesity-induced pro-inflammation in old-aged rats and helped to improve insulin sensitivity.
참고문헌 (39)
- Franceschi C, "Human immunosenescence: the prevailing of innate immunity, the failing of clonotypic immunity, and the filling of immunological space", Vaccine, 18, pp.1717~1720 (2000)
- Franceschi C, "Inflamm-aging. An evolutionary perspective on immunosenescence", Ann N Y Acad Sci, 908, pp.244~254 (2000)
- Suganami T, "Adipose tissue macrophages : their role in adipose tissue remodeling", J Leukoc Biol, 88, pp.33~39 (2010)
- "Obesity and Overweight. Fact Sheet" (2016)[URL]
- Rocha VZ, "Obesity, inflammation, and atherosclerosis", Nat Rev Cardiol, 6, pp.399~409 (2009)
- Lumeng CN, "Obesity induces a phenotypic switch in adipose tissue macrophage polarization", J Clin Invest, 117, pp.175~184 (2007)
- Leen Catrysse, "Adipose tissue macrophages and their polarization in health and obesity", Cellular Immunology, 330, pp.114~119 (2018), Elsevier BV[DOI]
- N. Satoh, "Unbalanced M1", M2 Phenotype of Peripheral Blood Monocytes in Obese Diabetic Patients: Effect of pioglitazone, 2009, pp.33(1), e7~e7[DOI]
- Bouhlel MA, "PPARactivation primes human monocytes into alternative M2macrophages with anti-inflammatory properties", Cell Metab, 6, pp.137~143 (2007)
- Odegaard JI, "Alternative M2 activation of Kupffer cells by PPARdelta ameliorates obesity-induced insulin resistance", Cell Metab, 7, pp.496~507 (2008)
- Jorming Goh, "Exercise and Adipose Tissue Macrophages: New Frontiers in Obesity Research?", Frontiers in Endocrinology, 7 (2016), Frontiers Media SA[DOI]
- Hancock CR, "High-fat diets cause insulin resistance despite an increase in muscle mitochondria", Proc Natl Acad Sci, 105(22), pp.7815~7820 (2008)
- De Angelis K, "Exercise training changes autonomic cardiovascular balance in mice", J Appl Physiol, 96(6), pp.2174~2178 (2004)
- Holloszy JO, "Biochemical adaptations in muscle", J Biol Chem, 242(9), pp.2278~2282 (1967)
- Henriksen EJ, "The high-fat-fed lean Zucker rat: a spontaneous isocaloric model of fat-induced insulin resistance associated with muscle GSK-3 overactivity", Am J Physiol Regul Integr Comp Physiol, 294(6), pp.R1813~R1821 (2008)
- Margolis B, "Effect of phospholipase C-gamma overexpression on PDGFinduced second messengers and mitogenesis", Science, 248(4955), pp.607~610 (1990)
- Lowry EC, "Serum levels of orally administered penicillin", U S Armed Forces Med J, 2(2), pp.265~270 (1951)
- Odegaard JI, "Pleiotropic actions of insulin resistance and inflammation in metabolic homeostasis", Science, 339(6116), pp.172~177 (2013)
- Park J, "Obesity and cancer-mechanisms underlying tumour progression and recurrence", Nat Rev Endocrinol, 10(8), pp.455~465 (2014)
- Lackey DE, "Regulation of metabolism by the innate immune system", Nat Rev Endocrinol, 12(1), pp.15~28 (2016)
- McLaughlin T, "Role of innate and adaptive immunity in obesity-associated metabolic disease", J Clin Invest, 127(1), pp.5~13 (2017)
- Weisberg SP, "Obesity is associated with macrophage accumulation in adipose tissue", J Clin Invest, 112(12), pp.1796~1808 (2003)
- Rosen ED, "What we talk about when we talk about fat", Cell, 156(1-2), pp.20~44 (2014)
- Lee YS, "Inflammation is necessary for longterm but not short-term high-fat diet-induced insulin resistance", Diabetes, 60(10), pp.2474~2483 (2011)
- Tian J, "Oral treatment with γ-aminobutyric acid improves glucose tolerance and insulin sensitivity by inhibiting inflammation in high fat diet-fed mice", PloS ONE, 6(9), pp.e25338~ (2011)
- Mau T, "Adipose tissue inflammation in aging", Experimental Gerontology, 105, pp.27~31 (2018)
- Mitsugu Shimobayashi, "Insulin resistance causes inflammation in adipose tissue", Journal of Clinical Investigation, 128(4), pp.1538~1550 (2018), American Society for Clinical Investigation[DOI]
- Olefsky JM, "Macrophages, inflammation, and insulin resistance", Annu Rev Physiol, 72, pp.219~246 (2010)
- Lee B, "C", EBPα regulates macrophage activation and systemic metabolism, 2014, pp.306, E1144~E1154
- Odegaard JI, "Macrophage-specific PPARgamma controls alternative activation and improves insulin resistance", Nature, 447, pp.1116~1120 (2007)
- Lumeng CN, "Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes", Diabetes, 57, pp.3239~3246 (2008)
- Weisberg SP, "CCR2 modulates inflammatory and metabolic effects of high-fat feeding", J Clin Invest, 116, pp.115~124 (2006)
- Dandona P, "Tumor necrosis factor-alpha in sera of obese patients : fall with weight loss", J Clin Endocrinol Metab, 83, pp.2907~2910 (1998)
- Mason AE, "Weight loss maintenance and cellular aging in the supporting health through nutrition and exercise study", Psychosomatic Medicine, 80(7), pp.609~619 (2018)[DOI]
- Oliveira AG, "Physical exercise reduces circulating lipopolysaccharide and TLR4activation and improves insulin signaling in tissues of DIO rats", Diabetes, 60, pp.784~796 (2011)
- Xu X, "Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue", Am J Physiol, 300, pp.R1115~R1125 (2011)
- Bruun JM, "Diet and exercise reduce lowgrade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects", Am J Physiol Endocrinol Metab, 290, pp.E961~E967 (2006)
- Alexandre G. Oliveira, "Acute exercise induces a phenotypic switch in adipose tissue macrophage polarization in diet-induced obese rats", Obesity, 21(12), pp.2545~2556 (2013), Wiley[DOI]
- Silveira LS, "Exercise rescues the immune response fine-tuned impaired by peroxisome proliferator-activated receptors γdeletion in macrophages", J Cell Physiol, 234(4), pp.5241~5251 (2019)
