Metabolite Profiling and Microbial Community of Traditional Meju Show Primary and Secondary Metabolite Differences Correlated with Antioxidant Activities
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Meju, a type of fermented soybean paste, is used as a starter in the preparation of various Korean traditional soybean-based foods. In this study, we performed Illumina-MiSeq paired-end sequencing for microbial communities and mass spectrometry analysis for metabolite profiling to investigate the differences between 11 traditional meju products from different regions across Korea. Even though the bacterial and fungal communities showed remarkable variety, major genera including Bacillus, Enterococcus, Variovorax, Pediococcus, Weissella, and Aspergillus were detected in every sample of meju. The metabolite profile patterns of the 11 samples were clustered into two main groups: group I (M1-5) and group II (M6-11). The metabolite analysis indicated a relatively higher amino acid content in group I, while group II exhibited higher isoflavone, soyasaponin, and lysophospholipid contents. The bioactivity analysis proved that the ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)) radical-scavenging activity was higher in group II and the FRAP (ferric reducing antioxidant power) activity was higher in group I. The correlation analysis revealed that the ABTS activity was isoflavonoid, lipid, and soyasaponin related, whereas the FRAP activity was amino acid and flavonoid related. These results suggest that the antioxidant activities of meju are critically influenced by the microbiome and metabolite dynamics.
Abstract
Meju, a type of fermented soybean paste, is used as a starter in the preparation of various Korean traditional soybean-based foods. In this study, we performed Illumina-MiSeq paired-end sequencing for microbial communities and mass spectrometry analysis for metabolite profiling to investigate the differences between 11 traditional meju products from different regions across Korea. Even though the bacterial and fungal communities showed remarkable variety, major genera including Bacillus, Enterococcus, Variovorax, Pediococcus, Weissella, and Aspergillus were detected in every sample of meju. The metabolite profile patterns of the 11 samples were clustered into two main groups: group I (M1-5) and group II (M6-11). The metabolite analysis indicated a relatively higher amino acid content in group I, while group II exhibited higher isoflavone, soyasaponin, and lysophospholipid contents. The bioactivity analysis proved that the ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)) radical-scavenging activity was higher in group II and the FRAP (ferric reducing antioxidant power) activity was higher in group I. The correlation analysis revealed that the ABTS activity was isoflavonoid, lipid, and soyasaponin related, whereas the FRAP activity was amino acid and flavonoid related. These results suggest that the antioxidant activities of meju are critically influenced by the microbiome and metabolite dynamics.
참고문헌 (44)
- Lee S, "Comparative evaluation of microbial diversity and metabolite profiles in doenjang, a fermented soybean paste, during the two different industrial manufacturing processes", Food Chem, 221, pp.1578~1586 (2017)
- Lee EM, "Highly geographical specificity of metabolomic traits among Korean domestic soybeans(Glycine max)", Food Res. Int, 120, pp.12~18 (2019)
- Iqbal N, "Soybean (Glycine max L.) germplasm screening and geographical determination based on targeted isoflavone metabolomics", Appl. Ecol. Env. Res., 16, pp.3933~3953 (2018)
- Yokota T, "The effect of antioxidant-containing fraction from fermented soybean food on atherosclerosis development in cholesterol-fed rabbits", LWT-Food Sci. Technol, 29, pp.751~755 (1996)
- Donghwa Shin, "Korean traditional fermented soybean products: Jang", Journal of Ethnic Foods, 2(1), pp.2~7 (2015), 한국식품연구원
- Shukla S, "Determination of non-volatile and volatile organic acids in Korean traditional fermented soybean paste(Doenjang)", Food Chem. Toxicol, 48, pp.2005~2010 (2010)
- Kum S-J, "Effects of Aspergillus species inoculation and their enzymatic activities on the formation of volatile components in fermented soybean paste(doenjang)", J. Agric. Food Chem, 63, pp.1401~1418 (2015)
- Lee JH, "Determination of microbial diversity in meju, fermented cooked soya beans, using nested PCR‐denaturing gradient gel electrophoresis", Lett. Appl. Microbiol, 51, pp.388~394 (2010)
- Yi-Seul Kim, "Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods", The Journal of Microbiology, 49(3), pp.340~348 (2011), 한국미생물학회
- Wishart DS, "Metabolomics : applications to food science and nutrition research", Trends Food Sci. Technol, 19, pp.482~493 (2008)
- Lee S, "Targeted metabolomics for Aspergillus oryzae-mediated biotransformation of soybean isoflavones, showing variations in primary metabolites", Biosci. Biotechnol. Biochem, 78, pp.167~174 (2014)
- Ko B-K, "Metabolomic insight into soy sauce through 1H NMR spectroscopy", J. Agric. Food Chem, 57, pp.6862~6870 (2009)
- Chun BH, "The effect of salt concentrations on the fermentation of doenjang, a traditional Korean fermented soybean paste", Food Microbiol, 86, pp.103329~ (2020)
- Lee S, "Chemotaxonomic metabolite profiling of 62 indigenous plant species and its correlation with bioactivities", Molecules, 20, pp.19719~19734 (2015)
- Son SY, "Metabolite fingerprinting, pathway analyses, and bioactivity correlations for plant species belonging to the Cornaceae, Fabaceae, and Rosaceae families", Plant Cell Rep, 35, pp.1917~1931 (2016)
- Su Yun Lee, "Mass Spectrometry-Based Metabolite Profiling and Bacterial Diversity Characterization of Korean Traditional Meju During Fermentation", Journal of Microbiology and Biotechnology, 22(11), pp.1523~1531 (2012), 한국미생물·생명공학회[DOI]
- Xie M, "An integrated metagenomic", metaproteomic investigation of microbiota in dajiang-meju, a traditional fermented soybean product in Northeast China, 2019, pp.115, 414~424
- Kang HJ, "Metabolomic analysis of meju during fermentation by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS)", Food Chem, 127, pp.1056~1064 (2011)
- Jung WY, "Functional characterization of bacterial communities responsible for fermentation of Doenjang : a traditional Korean fermented soybean paste", Front. Microbiol, 7, pp.827~ (2016)
- Oh D-G, "Metabolomics reveals the effect of garlic on antioxidant-and protease-activities during Cheonggukjang(fermented soybean paste)fermentation", Food Res. Int, 82, pp.86~94 (2016)
- Shin GR, "Metabolomics reveal that amino acids are the main contributors to antioxidant activity in wheat and rice gochujangs(Korean fermented red pepper paste)", Food Res. Int, 87, pp.10~17 (2016)
- Lee GM, "Metabolomics provides quality characterization of commercial gochujang(fermented pepper paste)", Molecules, 21, pp.921~ (2016)
- Kim D-H, "Fermentation characteristics of whole soybean meju model system inoculated with 4Bacillus strains", Korean J. Food Sci. Technol, 29, pp.1006~1015 (1997)
- Jung JY, "Microbial community dynamics during fermentation of doenjang-meju, traditional Korean fermented soybean", Int. J. Food Microbiol, 185, pp.112~120 (2014)
- Nogueira M, "Mycorrhiza and soil bacteria influence extractable iron and manganese in soil and uptake by soybean", Plant Soil, 298, pp.273~284 (2007)
- Rhyu MR, "Umami taste characteristics of water extract of Doenjang, a Korean soybean paste:Low-molecular acidic peptides may be a possible clue to the taste", Food Chem., 127, pp.1210~1215 (2011)
- Solms J, "Taste of amino acids, peptides, and proteins", J. Agric. Food Chem, 17, pp.686~688 (1969)
- Namgung HJ, "Metabolite profiling of doenjang, fermented soybean paste, during fermentation", J. Sci. Food Agric, 90, pp.1926~1935 (2010)
- Lee SY, "Primary and secondary metabolite profiling of doenjang, a fermented soybean paste during industrial processing", Food Chem, 165, pp.157~166 (2014)
- Dalluge JJ, "Simultaneous identification of soyasaponins and isoflavones and quantification of soyasaponin Bb in soy products, using liquid chromatography", electrospray ionization-mass spectrometry, 2003, pp.51, 3520~3524
- Rostagno MA, "Sample preparation for the analysis of isoflavones from soybeans and soy foods", J. Chromatogr. A, 1216, pp.2~29 (2009)
- Křížová L, "Isoflavones", Molecules, 24, pp.1076~ (2019)
- Chan Ho Jang, "Metabolism of Isoflavone Derivatives During Manufacturing of Traditional Meju and Doenjang", Food Science and Biotechnology, 17(2), pp.442~445 (2008), 한국식품과학회
- Kwon DY, "Isoflavonoids and peptides from meju, long-term fermented soybeans, increase insulin sensitivity and exert insulinotropic effects in vitro", Nutrition, 27, pp.244~252 (2011)
- Berhow MA, "Complete quantification of group A and group B soyasaponins in soybeans", J. Agric. Food Chem, 54, pp.2035~2044 (2006)
- Okubo K, "Components responsible for the undesirable taste of soybean seeds", Biosci. Biotechnol. Biochem, 56, pp.99~103 (1992)
- Hong S-W, "Soyasaponin I improved neuroprotection and regeneration in memory deficient model rats", PLoS One, 8, pp.e81556~ (2013)
- Koratkar R, "Effect of soya bean saponins on azoxymethane‐induced preneoplastic lesions in the colon of mice", Nutr. Cancer, 27, pp.206~209 (1997)
- Jakubczyk K, "Antioxidant properties and nutritional composition of matcha green tea", Foods, 9, pp.483~ (2020)
- Lee JH, "Comparative analyses of total phenols, flavonoids, saponins and antioxidant activity in yellow soy beans and mung beans", Int. J. Food Sci. Technol, 46, pp.2513~2519 (2011)
- Pannala AS, "Flavonoid B-ring chemistry and antioxidant activity : fast reaction kinetics", Biochem. Biophys. Res. Commun, 282, pp.1161~1168 (2001)
- Okubo K, "Oxygen-radical-scavenging activity of DDMP-conjugated saponins and physiological role in leguminous plant, Saponins used in food and agriculture", pp.141~154 (1996), Springer
- Chirinang P, "Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju", Sci. Asia, 35, pp.326~331 (2009)
- Martínez‐Valverde I, "Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato(Lycopersicum esculentum)", J. Sci. Food Agric, 82, pp.323~330 (2002)
