LC-HRMS를 이용한 송사리 내 의약품 농축 및 생체변환 연구
Bioconcentration and Biotransformation of Pharmaceuticals in Oryzias latipes using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The occurrence of pharmaceuticals in the aquatic environment has been increasing steadily owing to the increasing use of pharmaceuticals in our daily life. This results in increasing challenges of environmental health risk as pharmaceuticals in the surface water can be accumulated and transformed in aquatic organisms. The purpose of this study was to predict the bioconcentration and biotransformation of several pharmaceuticals in Japanese medaka (Oryzias latipes) using a 96 h exposure test. Based on an investigation of the most frequently detected pharmaceuticals in the surface waters in South Korea, 11 target compounds were selected including atenolol, caffeine, carbamazepine, diclofenac, fluoxetine, irbesartan, losartan, mefenamic acid, metoprolol, naproxen, and venlafaxine. A bioconcentration factor of 1.9, 31.3, and 10.7 was expected in fish owing to the accumulation of carbamazepine, fluoxetine, and mefenamic acid, respectively. A total of 12 biotransformation products (BTPs) were tentatively identified via oxidation, hydroxylation, dealkylation, and demethylation reactions. In summary, it is expected that these BTPs represented by molecular structures derived from their parent compounds can be utilized to evaluate the change in toxicity of BTPs compared to that of the parent compounds.
Abstract
The occurrence of pharmaceuticals in the aquatic environment has been increasing steadily owing to the increasing use of pharmaceuticals in our daily life. This results in increasing challenges of environmental health risk as pharmaceuticals in the surface water can be accumulated and transformed in aquatic organisms. The purpose of this study was to predict the bioconcentration and biotransformation of several pharmaceuticals in Japanese medaka (Oryzias latipes) using a 96 h exposure test. Based on an investigation of the most frequently detected pharmaceuticals in the surface waters in South Korea, 11 target compounds were selected including atenolol, caffeine, carbamazepine, diclofenac, fluoxetine, irbesartan, losartan, mefenamic acid, metoprolol, naproxen, and venlafaxine. A bioconcentration factor of 1.9, 31.3, and 10.7 was expected in fish owing to the accumulation of carbamazepine, fluoxetine, and mefenamic acid, respectively. A total of 12 biotransformation products (BTPs) were tentatively identified via oxidation, hydroxylation, dealkylation, and demethylation reactions. In summary, it is expected that these BTPs represented by molecular structures derived from their parent compounds can be utilized to evaluate the change in toxicity of BTPs compared to that of the parent compounds.
참고문헌 (26)
- OECD, "OECD Health Statistics 2020", 1~38 (2020)
- 전준호, "고분해능질량분석기 기반의 표적, 추정, 비표적 분석기법을 활용한 수환경 미량오염물질 및 환경변환체 탐색: A review", 환경분석과 독성보건, 19(4), pp.225~245 (2016), 한국환경분석학회
- M. A. Aukidy, "Monitoring release of pharmaceutical compounds : Occurrence and environmental risk assessment of two WWTP effluents and their receiving bodies in the Po Valley, Italy", Science of the Total Environment, 438, pp.15~25 (2012)
- N. Collado, "Pharmaceuticals occurrence in a WWTP with significant industrial contribution and its input into the river system", Environmental Pollution, 185, pp.202~212 (2014)
- E. Gracia-Lor, "Multi-class determination of personal care products and pharmaceuticals in environmental and wastewater samples by ultra-high performance liquid-chromatography-tandem mass spectrometry", Talanta, 99, pp.1011~1023 (2012)
- P. Kay, "Widespread, routine occurrence of pharmaceuticals in sewage effluent, combined sewer overflows and receiving waters", Environmental Pollution, 220, pp.1447~1455 (2017)
- G. Š. Parezanović, "Environmental Transformation of Pharmaceutical Formulations : A Scientific Review", Archives of Environmental Contamination and Toxicology, 77, pp.155~161 (2019)
- 남선화, "국내 생물종을 이용한 생태독성평가 기반연구 : ( I ) 어류", 생태와 환경, 40(2), pp.173~183 (2007), 한국하천호수학회
- 전준호, "LC-HRMS를 이용한 Daphnia magna 및 Gammarus pulex 생체내 의약품 대사체 정성분석", 환경분석과 독성보건, 21(4), pp.243~251 (2018), 한국환경분석학회
- J. Hollender, "Nontarget Screening with High Resolution Mass Spectrometry in the Environment : Ready to Go?", Environmental Science & Technology, 51, pp.11505~11512 (2017)
- J. Martín, "Occurrence of pharmaceutical compounds in wastewater and sludge from wastewater treatment plants : Removal and ecotoxicological impact of wastewater discharges and sludge disposal", Journal of Hazardous Materials, 239-240, pp.40~47 (2012)
- USEPA, "Developing the risk-screening environmental indicators", ~ (2017)
- E. L. Schymanski, "Identifying Small Molecules via High Resolution Mass Spectrometry : Communicating Confidence", Environmental Science &Technology, 48, pp.2097~2098 (2014)
- L. E. Gómez-Martínez, "Disposition Kinetics of Caffeine and Paraxanthine in Nile Tilapia(Oreochromis niloticus) : Characterization of the Main Metabolites", Archives of Environmental Contamination and Toxicology, 60, pp.654~664 (2011)
- S. N. Garcia, "Field and laboratory fish tissue accumulation of the anti-convulsant drug carbamazepine", Ecotoxicology and Environmental Safety, 84, pp.207~211 (2012)
- J. Liu, "Occurrence, bioaccumulation and risk assessment of lipophilic pharmaceutically active compounds in the downstream rivers of sewage treatment plants", Science of the Total Environment, 511, pp.54~62 (2015)
- Y. Nakamura, "The effects of pH on fluoxetine in Japanese medaka(Oryzias latipes) : Acute toxicity in fish larvae and bioaccumulation in juvenile fish", Chemosphere, 70, pp.865~873 (2008)
- M. E. Valdés, "Bioaccumulation and bioconcentration of carbamazepine and other pharmaceuticals in fish under field and controlled laboratory experiments. Evidences of carbamazepine metabolization by fish", Science of the Total Environment, 557-558, pp.58~67 (2016)
- J. Radjenović, "Identification and structural characterization of biodegradation products of atenolol and glibenclamide by liquid chromatography coupled to hybrid quadrupole time-of-flight and quadrupole ion trap mass spectrometry", Journal of Chromatography A, 1210, pp.142~153 (2008)
- J. Aceña, "Metabolite profiling of carbamazepine and ibuprofen in Solea senegalensis bile using high-resolution mass spectrometry", Analytical and Bioanalytical Chemistry, 409(23), pp.5441~5450 (2017)
- C. Boillot, "In vivo exposure of marine mussels to carbamazepine and 10-hydroxy-10, 11-dihydro-carbamaze-pine : Bioconcentration and metabolization", Science of the Total Environment, 532, pp.564~570 (2015)
- J. M. Kallio, "Metabolites of the Aquatic Pollutant Diclofenac in Fish Bile", Environmental Science & Technology, 44, pp.7213~7219 (2010)
- F. Zindler, "Norfluoxetine Is the Only Metabolite of Fluoxetine in Zebrafish(Danio rerio)Embryos That Accumulates at Environmentally Relevant Exposure Scenarios", Environmental Science & Technology, 54, pp.4200~4209 (2020)
- S. Chu, "Analysis of paroxetine, fluoxetine and norfluoxetine in fish tissues using pressurized liquid extraction, mixed mode solid phase extraction cleanup and liquid chromatography-tandem mass spectrometry", Journal of Chromatography A, 1163, pp.112~118 (2007)
- Y. Choi, "Characterizing biotransformation products and pathways of the flame retardant triphenyl phosphate in Daphnia magna using non-target screening", Science of the Total Environment, 708, pp.135106~ (2020)
- J. Jeon, "Biotransformation pathways of biocides and pharmaceuticals in freshwater crustaceans based on structure elucidation of metabolites using high resolution mass spectrometry", Chemical Research in Toxicology, 26, pp.313~324 (2013)